Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACHMXC_RS10105 Genome accession   NZ_CP171997
Coordinates   2162153..2163196 (-) Length   347 a.a.
NCBI ID   WP_015012257.1    Uniprot ID   A0A8I1V4A6
Organism   Acidovorax delafieldii strain UC81_30     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2157153..2168196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXC_RS10075 (ACHMXC_10070) - 2157288..2157683 (+) 396 Protein_1978 YraN family protein -
  ACHMXC_RS10080 (ACHMXC_10075) - 2157774..2158373 (+) 600 WP_056059914.1 SIS domain-containing protein -
  ACHMXC_RS10085 (ACHMXC_10080) - 2158370..2159035 (+) 666 WP_108498071.1 BON domain-containing protein -
  ACHMXC_RS10090 (ACHMXC_10085) - 2159241..2160155 (-) 915 WP_056059909.1 NAD(P)-dependent oxidoreductase -
  ACHMXC_RS10095 (ACHMXC_10090) pilU 2160255..2161391 (-) 1137 WP_056059906.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHMXC_RS10100 (ACHMXC_10095) - 2161450..2162085 (-) 636 WP_015012256.1 Crp/Fnr family transcriptional regulator -
  ACHMXC_RS10105 (ACHMXC_10100) pilT 2162153..2163196 (-) 1044 WP_015012257.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHMXC_RS10110 (ACHMXC_10105) - 2163234..2163953 (+) 720 WP_395457724.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHMXC_RS10115 (ACHMXC_10110) ltaE 2163960..2165030 (-) 1071 WP_395457726.1 low-specificity L-threonine aldolase -
  ACHMXC_RS10120 (ACHMXC_10115) - 2165140..2166387 (-) 1248 WP_395457728.1 branched-chain amino acid ABC transporter substrate-binding protein -
  ACHMXC_RS10125 (ACHMXC_10120) - 2166447..2167097 (-) 651 WP_395457730.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38134.90 Da        Isoelectric Point: 6.7743

>NTDB_id=1064756 ACHMXC_RS10105 WP_015012257.1 2162153..2163196(-) (pilT) [Acidovorax delafieldii strain UC81_30]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKTYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNESEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1064756 ACHMXC_RS10105 WP_015012257.1 2162153..2163196(-) (pilT) [Acidovorax delafieldii strain UC81_30]
GTGGACATTACCCAATTGCTCGCGTTCAGCGTGAAGAACAAGGCCTCCGACCTGCACCTCTCCGCCGGTCTACCGCCCAT
GATCCGGGTCCACGGCGACGTGCGGCGTATCAACGTCGATGCGCTGGACCACAAGACGGTGCATGCCATGGTGTACGACA
TCATGAGCGACGCGCAGCGCAAGACGTATGAAGAGTTCCTGGAGGTGGACTTTTCCTTCGAGATCGAAGGCCTGGCCCGC
TTCCGCGTCAATGCCTTCAACCAGAACCGGGGCGCGGCCGCCGTGTTCCGGACGATTCCAAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCCCCCAAGATCTTTGGCGATCTGGCTTTGAAGCCACGGGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGCCATGGTCAACTACCTCAACGAATCCGAATACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGTGAAGTCGGCCCGATGACGCTGTCCTTCGCGGC
GGCCCTGAAGTCCGCACTGCGCGAAGACCCGGACGCCATTCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACGGCTGCTGAAACGGGCCACTTGGTGTTTGGCACGCTGCACACCTCGAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGCCGAAGAAAAGGAAATGGTCCGTGCGATGTTGTCCGAATCACTGCAGGCCGTGATCTCGCAAAC
GCTGTGCAAGACCAAGGACGGCTCCGGCCGCGTGGCTGCGCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTGGGCATGCAGACACTGGACCAGAAC
CTGACCGACCTCGTGCGCCGCAACATCATCAGTCCCGCCGAAGCACGCAGCAAGGCCAAGATCCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment