Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACHMXC_RS10095 Genome accession   NZ_CP171997
Coordinates   2160255..2161391 (-) Length   378 a.a.
NCBI ID   WP_056059906.1    Uniprot ID   A0A2G6SEP0
Organism   Acidovorax delafieldii strain UC81_30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2155255..2166391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXC_RS10070 (ACHMXC_10065) rsmI 2156345..2157274 (-) 930 WP_395457721.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACHMXC_RS10075 (ACHMXC_10070) - 2157288..2157683 (+) 396 Protein_1978 YraN family protein -
  ACHMXC_RS10080 (ACHMXC_10075) - 2157774..2158373 (+) 600 WP_056059914.1 SIS domain-containing protein -
  ACHMXC_RS10085 (ACHMXC_10080) - 2158370..2159035 (+) 666 WP_108498071.1 BON domain-containing protein -
  ACHMXC_RS10090 (ACHMXC_10085) - 2159241..2160155 (-) 915 WP_056059909.1 NAD(P)-dependent oxidoreductase -
  ACHMXC_RS10095 (ACHMXC_10090) pilU 2160255..2161391 (-) 1137 WP_056059906.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHMXC_RS10100 (ACHMXC_10095) - 2161450..2162085 (-) 636 WP_015012256.1 Crp/Fnr family transcriptional regulator -
  ACHMXC_RS10105 (ACHMXC_10100) pilT 2162153..2163196 (-) 1044 WP_015012257.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHMXC_RS10110 (ACHMXC_10105) - 2163234..2163953 (+) 720 WP_395457724.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHMXC_RS10115 (ACHMXC_10110) ltaE 2163960..2165030 (-) 1071 WP_395457726.1 low-specificity L-threonine aldolase -
  ACHMXC_RS10120 (ACHMXC_10115) - 2165140..2166387 (-) 1248 WP_395457728.1 branched-chain amino acid ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41864.24 Da        Isoelectric Point: 6.9078

>NTDB_id=1064755 ACHMXC_RS10095 WP_056059906.1 2160255..2161391(-) (pilU) [Acidovorax delafieldii strain UC81_30]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTSNHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGKVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQALFDAYEANVISYEDALRNADSLNDLRLQIKLNSQRAKSPDLAQGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1064755 ACHMXC_RS10095 WP_056059906.1 2160255..2161391(-) (pilU) [Acidovorax delafieldii strain UC81_30]
ATGGAACGCGATCAGGCCAGTAAATTCATCAATGACCTGCTCAAGCTGATGGTGAGCCGCAACGGCAGCGACTTGTTCAT
CACGGCAGAGTTTCCACCCGCCATCAAGGTCGATGGCAAGGTGACCAAGGTGTCGCCGCAACCCCTCACGTCCAACCACA
CGCTCACGCTGGCGCGCGCCATCATGAGCGACAAGCAGGTGGCGGACTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCGCCCGCTGGCATCGGGCGCTTCCGCGTCAACGCTTTCATCCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
GCTCACGCTGCCCACCATCGACGGCCTGGGTGTGCCGCAGGTGCTCAAGGAAGTGACGATGACCAAACGCGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAACTCGTTCGGC
CACATCATCACGGTGGAAGACCCCATCGAATTTGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGTGAAGTGGGCCT
GGATACCGACAGCTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGATGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAACATGCGGTGGCGTTTGCCGAAACCGGGCACTTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGTGCCCAGTTGTTGATGGACCTGTCGCTCAACCT
GCGCGCCATGGTGTCGCAGCGCCTGATCCCCAAGCAGGACGGCAAAGGCCGCGCCGCAGCGGTGGAGGTGATGCTCAACA
CCCCACTGATCTCCGACCTGATCTTCAAGGGCGAGGTCTCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGATGCCTACGAGGCCAATGTCATCAGCTACGAAGACGCACTGCGCAATGCCGA
CTCGCTCAACGACTTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCCAAGTCACCCGACCTGGCACAGGGCACCGAGC
ATTTTGCGATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G6SEP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

58.824

94.444

0.556
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter baylyi ADP1

43.574

84.392

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365

Multiple sequence alignment