Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACHFJ9_RS20495 Genome accession   NZ_CP171887
Coordinates   2712851..2713957 (-) Length   368 a.a.
NCBI ID   WP_395356144.1    Uniprot ID   -
Organism   Vibrio sp. D3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2707851..2718957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFJ9_RS20465 (ACHFJ9_20465) - 2708482..2708979 (+) 498 WP_012841396.1 SprT family zinc-dependent metalloprotease -
  ACHFJ9_RS20470 (ACHFJ9_20470) endA 2709130..2709825 (+) 696 WP_005393967.1 deoxyribonuclease I -
  ACHFJ9_RS20475 (ACHFJ9_20475) rsmE 2709942..2710673 (+) 732 WP_005393970.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACHFJ9_RS20480 (ACHFJ9_20480) gshB 2710687..2711637 (+) 951 WP_395356141.1 glutathione synthase -
  ACHFJ9_RS20485 (ACHFJ9_20485) - 2711766..2712329 (+) 564 WP_005393973.1 YqgE/AlgH family protein -
  ACHFJ9_RS20490 (ACHFJ9_20490) ruvX 2712364..2712789 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  ACHFJ9_RS20495 (ACHFJ9_20495) pilU 2712851..2713957 (-) 1107 WP_395356144.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHFJ9_RS20500 (ACHFJ9_20500) pilT 2713978..2715018 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHFJ9_RS20505 (ACHFJ9_20505) - 2715047..2715748 (+) 702 WP_258661906.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHFJ9_RS20510 (ACHFJ9_20510) proC 2715906..2716724 (+) 819 WP_047008834.1 pyrroline-5-carboxylate reductase -
  ACHFJ9_RS20515 (ACHFJ9_20515) - 2716777..2717334 (+) 558 WP_012841393.1 YggT family protein -
  ACHFJ9_RS20520 (ACHFJ9_20520) yggU 2717334..2717624 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  ACHFJ9_RS20525 (ACHFJ9_20525) - 2717752..2718183 (+) 432 WP_005393982.1 DUF4426 domain-containing protein -
  ACHFJ9_RS20530 (ACHFJ9_20530) - 2718302..2718904 (+) 603 WP_046875112.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41301.46 Da        Isoelectric Point: 6.9726

>NTDB_id=1064660 ACHFJ9_RS20495 WP_395356144.1 2712851..2713957(-) (pilU) [Vibrio sp. D3]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIYMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1064660 ACHFJ9_RS20495 WP_395356144.1 2712851..2713957(-) (pilU) [Vibrio sp. D3]
ATGGATCTAAATAAATTTCTCGAAGGTATGCTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTTT
GTTTCGCGTGGATGGCGAATTACGACCTCAAGGTGAAAAACTCACCGAGAGTGATGTAGCGGCATTACTCGACAGTGCTA
TGGAGCCAGATCGGCGTCAGGAATTTCGTAAAAGTCGTGAGTCGAATTTTGCCATTGTGAGAGATTGTGGCCGCTTCCGT
GTTAGTGCCTTTTTTCAACGGGAATTACCGGGTGCGGTGATTCGTCGTATTGAAACCAATATTCCCACCTTTGAGCAATT
AAAGTTACCGTTAGTGTTGCAAGATTTAGCGATAGCCAAGCGTGGTTTGGTGCTGGTGGTTGGTGCTACTGGGTCAGGTA
AATCTACCACAATGGCGGCAATGACGGGCTATCGAAACAGCAACAAGACGGGACACATTTTGACGGTCGAAGATCCGATC
GAATTCGTGCATGAGCACAAGCGCTGTATCGTGACTCAACGCGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGTCAAGCGCCAGATATGATTTTGATTGGCGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CTTTCGCCGAAACGGGCCATTTGTGTATGGCAACGTTGCACGCCAACAATGCCAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGACTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGCTGAT
CCGAGATAAAAATGGGCAGGGGCGTCATGGTGTGTTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATCCGTC
GCGGAGATTTGCATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTGGTGATGCAAGGTAAGATCAGCGAAGAGGACGCGCTGCACAGTGCCGATTCGGCGAATGATTTGCGCTTGATGTT
GAAAACACAGCGTGGCGAAGCATTCTCTACGGGCAGTCTTGCTAACGTTAAGATCTATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.986

95.38

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361

Multiple sequence alignment