Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PN836_RS08360 Genome accession   NZ_CP171821
Coordinates   1908173..1909198 (-) Length   341 a.a.
NCBI ID   WP_395345554.1    Uniprot ID   -
Organism   Ningiella sp. W23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1903173..1914198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PN836_RS08330 (PN836_008460) gshB 1903443..1904405 (+) 963 WP_395344781.1 glutathione synthase -
  PN836_RS08335 (PN836_008465) - 1904418..1904993 (+) 576 WP_395344782.1 YqgE/AlgH family protein -
  PN836_RS08340 (PN836_008470) ruvX 1905017..1905442 (+) 426 WP_395344783.1 Holliday junction resolvase RuvX -
  PN836_RS08345 (PN836_008475) - 1905504..1905821 (-) 318 WP_395344784.1 DUF2007 domain-containing protein -
  PN836_RS08350 (PN836_008480) - 1906115..1906792 (+) 678 WP_395344785.1 hypothetical protein -
  PN836_RS08355 (PN836_008485) pilU 1907031..1908146 (-) 1116 WP_395345551.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PN836_RS08360 (PN836_008490) pilT 1908173..1909198 (-) 1026 WP_395345554.1 type IV pilus twitching motility protein PilT Machinery gene
  PN836_RS08365 (PN836_008495) - 1909305..1910030 (+) 726 WP_395344786.1 YggS family pyridoxal phosphate-dependent enzyme -
  PN836_RS08370 (PN836_008500) proC 1910020..1910841 (+) 822 WP_395344787.1 pyrroline-5-carboxylate reductase -
  PN836_RS08375 (PN836_008505) - 1910857..1911402 (+) 546 WP_395344788.1 YggT family protein -
  PN836_RS08380 (PN836_008510) - 1911457..1911903 (+) 447 WP_395344789.1 DUF4426 domain-containing protein -
  PN836_RS08385 (PN836_008515) - 1911909..1912541 (-) 633 WP_395344790.1 glutathione S-transferase family protein -
  PN836_RS08390 (PN836_008520) yaaA 1912551..1913336 (-) 786 WP_395344791.1 peroxide stress protein YaaA -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37911.41 Da        Isoelectric Point: 6.5038

>NTDB_id=1064407 PN836_RS08360 WP_395345554.1 1908173..1909198(-) (pilT) [Ningiella sp. W23]
MDINELLAFSVQNGASDLHLTAGLPPIIRVDGEMRRLNVDSLSHKNVHSLIYDIMNDKQRKEYEENLEVDFSFEIPELSR
FRVNAFNQNRGAAAVLRTIPSKVLTLDELGAPDIFKQIINQPTGIILVTGATGSGKSTTLAAMIDYINTHKREHILTIED
PIEFVHNNKLCVLNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKSMIRSMLSESLRAVISQTLLKKVGGGRVAAHEIMLGIPAIRNLIREDKVPQMYSVIQTGQSHGMQTMDQCL
QKLVAQGVITQQDAAARSTDK

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=1064407 PN836_RS08360 WP_395345554.1 1908173..1909198(-) (pilT) [Ningiella sp. W23]
TTGGATATTAACGAACTACTTGCTTTTAGCGTACAAAATGGCGCTTCCGATTTACACTTAACCGCGGGATTACCTCCAAT
TATACGTGTAGACGGTGAAATGCGTCGACTTAATGTCGATTCGCTTTCGCACAAAAATGTGCACAGCCTGATATACGACA
TTATGAACGATAAACAGCGTAAAGAGTACGAAGAAAACCTTGAAGTGGACTTTTCTTTCGAAATCCCCGAGCTATCACGT
TTCAGGGTCAATGCATTTAATCAAAACCGAGGCGCGGCGGCGGTATTGCGAACCATCCCCAGCAAAGTACTGACGCTAGA
TGAGCTAGGTGCGCCTGATATTTTCAAGCAAATTATTAACCAGCCTACGGGCATCATCTTGGTCACAGGGGCAACGGGGT
CAGGTAAAAGTACCACCTTGGCGGCAATGATAGACTATATCAACACGCACAAGCGTGAGCATATTCTGACCATTGAAGAT
CCCATCGAATTTGTTCACAACAATAAGCTTTGCGTGCTGAACCAGCGCGAAGTGCACCGCGATACACACAGCTTCAACAA
TGCTTTGCGAAGCGCCCTACGTGAAGATCCCGATGTAATACTCGTGGGTGAGCTACGTGATTTGGAAACGATTCGCCTTG
CCATTTCAGCCGCTGAAACCGGGCATTTGGTATTTGGTACCTTGCACACCAACTCTGCGCCTAAAACGATTGACAGAATA
ATTGATGTTTTTCCTGCTGAGGAAAAGTCCATGATCCGTTCTATGCTTTCAGAGTCTTTACGTGCGGTTATCTCTCAAAC
CCTGTTGAAGAAAGTCGGGGGTGGCCGTGTTGCAGCCCACGAAATCATGCTGGGGATCCCCGCTATCAGAAACTTAATTC
GTGAAGACAAAGTGCCGCAGATGTACTCAGTTATCCAAACAGGGCAATCGCATGGCATGCAAACCATGGATCAGTGTTTG
CAAAAGCTAGTGGCACAAGGTGTAATTACGCAGCAAGACGCCGCTGCACGTTCAACTGACAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.647

97.947

0.751
  pilT Acinetobacter baumannii strain A118

76.647

97.947

0.751
  pilT Acinetobacter nosocomialis M2

76.347

97.947

0.748
  pilT Acinetobacter baylyi ADP1

75.298

98.534

0.742
  pilT Pseudomonas aeruginosa PAK

73.669

99.12

0.73
  pilT Pseudomonas stutzeri DSM 10701

73.669

99.12

0.73
  pilT Legionella pneumophila strain Lp02

70.381

100

0.704
  pilT Legionella pneumophila strain ERS1305867

70.381

100

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

98.827

0.695
  pilT Vibrio cholerae strain A1552

70.326

98.827

0.695
  pilT Neisseria meningitidis 8013

65.396

100

0.654
  pilT Neisseria gonorrhoeae MS11

65.103

100

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.079

100

0.531
  pilU Acinetobacter baylyi ADP1

42.262

98.534

0.416
  pilU Pseudomonas stutzeri DSM 10701

42.388

98.24

0.416
  pilU Vibrio cholerae strain A1552

42.388

98.24

0.416

Multiple sequence alignment