Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PN836_RS08355 Genome accession   NZ_CP171821
Coordinates   1907031..1908146 (-) Length   371 a.a.
NCBI ID   WP_395345551.1    Uniprot ID   -
Organism   Ningiella sp. W23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1902031..1913146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PN836_RS08320 (PN836_008450) - 1902327..1902500 (+) 174 WP_395344779.1 hypothetical protein -
  PN836_RS08325 (PN836_008455) - 1902611..1903339 (+) 729 WP_395344780.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PN836_RS08330 (PN836_008460) gshB 1903443..1904405 (+) 963 WP_395344781.1 glutathione synthase -
  PN836_RS08335 (PN836_008465) - 1904418..1904993 (+) 576 WP_395344782.1 YqgE/AlgH family protein -
  PN836_RS08340 (PN836_008470) ruvX 1905017..1905442 (+) 426 WP_395344783.1 Holliday junction resolvase RuvX -
  PN836_RS08345 (PN836_008475) - 1905504..1905821 (-) 318 WP_395344784.1 DUF2007 domain-containing protein -
  PN836_RS08350 (PN836_008480) - 1906115..1906792 (+) 678 WP_395344785.1 hypothetical protein -
  PN836_RS08355 (PN836_008485) pilU 1907031..1908146 (-) 1116 WP_395345551.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PN836_RS08360 (PN836_008490) pilT 1908173..1909198 (-) 1026 WP_395345554.1 type IV pilus twitching motility protein PilT Machinery gene
  PN836_RS08365 (PN836_008495) - 1909305..1910030 (+) 726 WP_395344786.1 YggS family pyridoxal phosphate-dependent enzyme -
  PN836_RS08370 (PN836_008500) proC 1910020..1910841 (+) 822 WP_395344787.1 pyrroline-5-carboxylate reductase -
  PN836_RS08375 (PN836_008505) - 1910857..1911402 (+) 546 WP_395344788.1 YggT family protein -
  PN836_RS08380 (PN836_008510) - 1911457..1911903 (+) 447 WP_395344789.1 DUF4426 domain-containing protein -
  PN836_RS08385 (PN836_008515) - 1911909..1912541 (-) 633 WP_395344790.1 glutathione S-transferase family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40976.29 Da        Isoelectric Point: 5.8948

>NTDB_id=1064406 PN836_RS08355 WP_395345551.1 1907031..1908146(-) (pilU) [Ningiella sp. W23]
MTLDDCLKAMVDMDASDLFVTAGMPVSVKINGKLKPISDHRLSQEDALSLVHQAMNEEQIEDFKHEKECNFAVSLMDVGR
FRCSAFWQRDAAGMVVRRIVVEIPKASSLGLPPILNDIIMSKKGLVLFVGGTGTGKSTSLAALIGHRNENSYGHILTIED
PIEFLHSHRNCIVTQREVGIDTLSFDEALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERI
MHLAPKDLHAKLRYDLSQNIRAIVAQQLVPTIDGKGRVAAIEILLNSPLISDLIQRNEMGALKEAMMKGRELGMQTFDMA
LYDLYKLGKINEEQALHHADSPNDLRLMIKLDSKSFGSPLGSLSNVSIDLD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1064406 PN836_RS08355 WP_395345551.1 1907031..1908146(-) (pilU) [Ningiella sp. W23]
ATGACATTAGATGATTGTTTAAAAGCCATGGTAGACATGGATGCTTCTGACCTGTTTGTCACAGCAGGTATGCCAGTTAG
CGTGAAAATTAACGGCAAACTAAAACCGATTTCGGATCATCGTTTGAGTCAGGAAGATGCTTTATCTTTGGTTCATCAGG
CCATGAATGAAGAGCAAATTGAAGACTTCAAACATGAGAAAGAATGCAACTTTGCAGTTAGTTTGATGGATGTGGGGCGT
TTTCGTTGTTCCGCATTTTGGCAACGTGATGCGGCCGGCATGGTGGTGCGTCGTATTGTCGTTGAAATTCCAAAAGCGAG
TTCATTGGGGCTACCTCCAATTTTGAACGACATTATTATGTCGAAAAAGGGGCTAGTACTATTTGTTGGCGGCACCGGCA
CGGGTAAGTCTACGTCACTGGCAGCCTTAATTGGCCACCGTAATGAAAACTCGTATGGGCATATACTCACCATAGAAGAC
CCCATCGAGTTTTTGCATTCGCATCGCAATTGTATTGTGACGCAAAGGGAAGTAGGTATTGACACACTCTCGTTTGATGA
AGCGCTCAAAAGTTCGCTGCGTCAGGCGCCAGATGTCATTCTCATTGGCGAGATTCGTTCCATGGAGACCATGGAATATG
CGATGTCTTTTGCCGATACTGGGCATTTGTGTGTAGCAACCTTGCACGCAAATAATGCTAACCAAGCGATTGAACGTATC
ATGCACCTAGCGCCAAAAGACTTGCACGCGAAACTGCGATACGATTTAAGTCAAAATATTCGTGCTATTGTTGCTCAGCA
GTTAGTGCCCACTATTGACGGAAAAGGCCGTGTGGCTGCTATCGAAATTTTGCTTAACTCGCCACTCATTTCTGACTTGA
TTCAACGCAATGAAATGGGCGCGCTAAAAGAAGCAATGATGAAGGGCCGTGAGCTAGGTATGCAAACCTTTGATATGGCG
CTATATGATCTTTACAAGTTGGGCAAAATCAATGAAGAACAAGCGCTTCATCATGCCGACTCACCCAATGATTTGCGTTT
GATGATTAAACTGGACAGTAAATCATTCGGCAGTCCGTTAGGTTCATTAAGCAACGTATCGATTGATTTGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.615

98.113

0.585
  pilU Vibrio cholerae strain A1552

54.57

100

0.547
  pilU Acinetobacter baylyi ADP1

54.62

99.191

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.983

92.453

0.388
  pilT Vibrio cholerae strain A1552

38.483

95.957

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

38.483

95.957

0.369
  pilT Acinetobacter nosocomialis M2

40.058

92.183

0.369
  pilT Acinetobacter baumannii D1279779

40.058

92.183

0.369
  pilT Acinetobacter baumannii strain A118

40.058

92.183

0.369
  pilT Legionella pneumophila strain Lp02

40.597

90.296

0.367
  pilT Legionella pneumophila strain ERS1305867

40.597

90.296

0.367
  pilT Pseudomonas aeruginosa PAK

39.42

92.992

0.367

Multiple sequence alignment