Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACHFCC_RS15720 Genome accession   NZ_CP171806
Coordinates   3288495..3289538 (-) Length   347 a.a.
NCBI ID   WP_264892565.1    Uniprot ID   -
Organism   Schlegelella aquatica strain HS-12-14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3283495..3294538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFCC_RS15690 (ACHFCC_15690) - 3283758..3284126 (+) 369 WP_264892559.1 YraN family protein -
  ACHFCC_RS15695 (ACHFCC_15695) - 3284230..3284826 (+) 597 WP_264892560.1 phosphoheptose isomerase -
  ACHFCC_RS15700 (ACHFCC_15700) - 3284823..3285497 (+) 675 WP_395139558.1 BON domain-containing protein -
  ACHFCC_RS15705 (ACHFCC_15705) - 3285612..3286505 (-) 894 WP_395139560.1 NAD(P)-dependent oxidoreductase -
  ACHFCC_RS15710 (ACHFCC_15710) pilU 3286651..3287787 (-) 1137 WP_395139562.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHFCC_RS15715 (ACHFCC_15715) - 3287821..3288462 (-) 642 WP_264892564.1 cyclic nucleotide-binding domain-containing protein -
  ACHFCC_RS15720 (ACHFCC_15720) pilT 3288495..3289538 (-) 1044 WP_264892565.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHFCC_RS15725 (ACHFCC_15725) - 3289602..3290303 (+) 702 WP_395139565.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHFCC_RS15730 (ACHFCC_15730) - 3290345..3290827 (-) 483 WP_264892567.1 Lrp/AsnC ligand binding domain-containing protein -
  ACHFCC_RS15735 (ACHFCC_15735) putA 3290924..3293941 (+) 3018 WP_395139567.1 bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38162.94 Da        Isoelectric Point: 7.0600

>NTDB_id=1064299 ACHFCC_RS15720 WP_264892565.1 3288495..3289538(-) (pilT) [Schlegelella aquatica strain HS-12-14]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKQVHDMVYDIMNDAQRKAFEETLECDFSFEIQGLAR
FRVNAFNQSRGAGAVFRTIPSKILTLEQLNAPKVFADLALKPRGLVLVTGPTGSGKSTTLAAMINHLNENEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRAMLSESLVAVISQTLCKLKDGSGRVAAHEIMLGTSAIRNLIRENKIAQMYSAIQTGNSMGMQTLDQC
LADLVRRNLISPAEARAKAKFPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1064299 ACHFCC_RS15720 WP_264892565.1 3288495..3289538(-) (pilT) [Schlegelella aquatica strain HS-12-14]
ATGGACATCACGCAGCTGTTGGCCTTTTCGGTGAAGAACAAGGCTTCCGACTTGCACTTGTCGGCCGGGCTGCCCCCCAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAACGTCGACCCGTTGGACCACAAGCAGGTCCACGACATGGTCTACGACA
TCATGAACGATGCGCAGCGCAAGGCGTTCGAGGAGACGCTCGAGTGCGACTTCTCCTTCGAGATCCAAGGCCTCGCGCGC
TTTCGCGTCAACGCGTTCAACCAGAGCCGCGGCGCGGGCGCCGTCTTCCGTACGATTCCTTCCAAGATCCTCACGCTCGA
GCAGCTCAACGCCCCCAAGGTGTTCGCCGACCTCGCCCTCAAGCCCCGCGGGCTGGTCCTGGTGACCGGCCCCACCGGCT
CCGGCAAGTCGACGACGCTGGCCGCGATGATCAACCACCTCAACGAAAACGAGTACGGGCACATCCTCACCGTCGAGGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTAGGCCCGCACACCCTGAGCTTCAACAA
CGCGTTGCGCTCGGCGCTGCGCGAAGACCCGGACGCCATTCTCGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTCG
CGCTCACCGCGGCCGAGACGGGTCACCTCGTGTTCGGCACGCTGCACACCTCCAGCGCGGCCAAGACGATCGACCGCATC
GTGGACGTCTTCCCCGCCGCGGAGAAGGAGATGGTGCGGGCGATGCTCTCCGAGTCGCTCGTGGCCGTCATCTCTCAGAC
GCTGTGCAAGCTCAAGGACGGTTCGGGCCGCGTGGCCGCGCACGAGATCATGCTCGGCACCTCGGCCATCCGCAATCTGA
TCCGCGAGAACAAGATCGCACAGATGTACTCGGCCATCCAGACCGGCAACAGCATGGGCATGCAGACGCTCGACCAATGC
CTCGCCGACCTGGTGCGGCGCAACCTCATTTCTCCGGCGGAGGCGCGCGCGAAAGCCAAGTTCCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

72.566

97.695

0.709
  pilT Legionella pneumophila strain ERS1305867

72.566

97.695

0.709
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.864

97.406

0.651
  pilT Vibrio cholerae strain A1552

66.864

97.406

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

99.424

0.527
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409

Multiple sequence alignment