Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACHFCC_RS15710 Genome accession   NZ_CP171806
Coordinates   3286651..3287787 (-) Length   378 a.a.
NCBI ID   WP_395139562.1    Uniprot ID   -
Organism   Schlegelella aquatica strain HS-12-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3281651..3292787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFCC_RS15680 (ACHFCC_15680) - 3281980..3282780 (+) 801 WP_395139553.1 septal ring lytic transglycosylase RlpA family protein -
  ACHFCC_RS15685 (ACHFCC_15685) rsmI 3282812..3283765 (-) 954 WP_395139555.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACHFCC_RS15690 (ACHFCC_15690) - 3283758..3284126 (+) 369 WP_264892559.1 YraN family protein -
  ACHFCC_RS15695 (ACHFCC_15695) - 3284230..3284826 (+) 597 WP_264892560.1 phosphoheptose isomerase -
  ACHFCC_RS15700 (ACHFCC_15700) - 3284823..3285497 (+) 675 WP_395139558.1 BON domain-containing protein -
  ACHFCC_RS15705 (ACHFCC_15705) - 3285612..3286505 (-) 894 WP_395139560.1 NAD(P)-dependent oxidoreductase -
  ACHFCC_RS15710 (ACHFCC_15710) pilU 3286651..3287787 (-) 1137 WP_395139562.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHFCC_RS15715 (ACHFCC_15715) - 3287821..3288462 (-) 642 WP_264892564.1 cyclic nucleotide-binding domain-containing protein -
  ACHFCC_RS15720 (ACHFCC_15720) pilT 3288495..3289538 (-) 1044 WP_264892565.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHFCC_RS15725 (ACHFCC_15725) - 3289602..3290303 (+) 702 WP_395139565.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHFCC_RS15730 (ACHFCC_15730) - 3290345..3290827 (-) 483 WP_264892567.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42132.59 Da        Isoelectric Point: 6.5925

>NTDB_id=1064298 ACHFCC_RS15710 WP_395139562.1 3286651..3287787(-) (pilU) [Schlegelella aquatica strain HS-12-14]
MERDQASKFINDLLRLMIARNGSDLFLTADFPPAIKIDGRVTKVSPQPLNGQHTMALARAIMNDKQAAEFERTKECNFAI
APQGIGRFRVNAFMQQGHVGMVLRVIPQTLPTIDALGLPQVLKEIAMTKRGLVIMVGATGSGKSTTLAAMVDYRNENSYG
HIITIEDPVEFVHPHKNCIVTQREVGIDTDGWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKAMVSQRLLPRQEGKGRIAAVEILLNTPLISELIFKGEVAEIKEIMKKSRELG
MQTFDQSLFDLYEGNFITYEDALRNADSVNDLRLQIKLNSQRARSADLSAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1064298 ACHFCC_RS15710 WP_395139562.1 3286651..3287787(-) (pilU) [Schlegelella aquatica strain HS-12-14]
ATGGAACGTGATCAGGCATCCAAGTTCATCAACGACCTGTTGCGCCTCATGATCGCGCGCAACGGATCGGACCTGTTCCT
CACCGCCGACTTCCCGCCAGCGATCAAGATCGACGGGCGGGTCACCAAGGTCTCGCCGCAGCCGCTCAACGGTCAGCACA
CGATGGCGCTGGCGCGCGCCATCATGAACGACAAGCAGGCGGCGGAGTTCGAGCGCACGAAGGAGTGCAACTTCGCGATC
GCCCCGCAGGGCATCGGACGTTTCCGCGTCAACGCGTTCATGCAGCAGGGTCACGTGGGCATGGTGCTGCGCGTGATCCC
GCAGACGCTGCCGACCATCGACGCCCTGGGGCTGCCGCAGGTGCTCAAGGAGATCGCGATGACCAAGCGCGGCCTGGTCA
TCATGGTGGGCGCCACCGGCTCGGGCAAGTCCACCACGCTCGCTGCGATGGTGGACTACCGCAACGAGAATTCCTACGGC
CACATCATCACGATCGAGGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCATCGTCACCCAGCGCGAGGTGGGCAT
CGACACCGACGGCTGGGAGGCCGCGCTCAAGAACACGCTGCGCCAGGCACCCGACGTGATCCTCATGGGCGAGATCCGCG
ATCGCGAGACGATGGAGCACGCGGTGGCCTTCGCCGAAACGGGCCACCTGTGCATGGCCACGCTGCATGCCAACAGCGCC
AACCAGGCGCTGGATCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGCGCAACTCCTGATGGATCTGTCGCTCAACCT
CAAGGCCATGGTGTCGCAGCGGCTGCTGCCGCGGCAGGAGGGCAAGGGTCGCATTGCGGCGGTCGAGATCCTCCTCAACA
CCCCGCTCATCTCCGAGCTGATCTTCAAGGGCGAGGTGGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCGAGCTGGGC
ATGCAGACGTTCGACCAGTCGCTGTTCGACCTCTACGAAGGCAACTTCATCACCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGGTTGCAGATCAAGCTCAACAGCCAGCGGGCCCGCAGCGCCGATCTGTCGGCCGGCACCGAGC
ACCTCACCATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.115

96.825

0.611
  pilU Acinetobacter baylyi ADP1

58.774

94.974

0.558
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.071

89.153

0.429
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Pseudomonas stutzeri DSM 10701

44.51

89.153

0.397
  pilT Acinetobacter baumannii D1279779

44.745

88.095

0.394
  pilT Acinetobacter baumannii strain A118

44.745

88.095

0.394
  pilT Acinetobacter nosocomialis M2

44.745

88.095

0.394
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Acinetobacter baylyi ADP1

44.144

88.095

0.389
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.969

84.127

0.378
  pilT Vibrio cholerae strain A1552

44.969

84.127

0.378

Multiple sequence alignment