Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGHX3_RS04370 Genome accession   NZ_CP171632
Coordinates   981706..982743 (+) Length   345 a.a.
NCBI ID   WP_162339156.1    Uniprot ID   -
Organism   Pseudoxanthomonas japonensis strain LY0623     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 976706..987743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHX3_RS04335 (ACGHX3_04335) pdxH 976987..977589 (+) 603 WP_394697238.1 pyridoxamine 5'-phosphate oxidase -
  ACGHX3_RS04340 (ACGHX3_04340) aac(6') 977640..978035 (+) 396 WP_394697239.1 aminoglycoside 6'-N-acetyltransferase -
  ACGHX3_RS04345 (ACGHX3_04345) - 978038..978832 (+) 795 WP_394697240.1 cobalamin-binding protein -
  ACGHX3_RS04350 (ACGHX3_04350) - 978994..979446 (-) 453 WP_394696524.1 DUF4426 domain-containing protein -
  ACGHX3_RS04355 (ACGHX3_04355) proC 979491..980330 (-) 840 WP_394696525.1 pyrroline-5-carboxylate reductase -
  ACGHX3_RS04360 (ACGHX3_04360) - 980339..980914 (-) 576 WP_394696526.1 hypothetical protein -
  ACGHX3_RS04365 (ACGHX3_04365) - 980935..981612 (-) 678 WP_394696527.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHX3_RS04370 (ACGHX3_04370) pilT 981706..982743 (+) 1038 WP_162339156.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHX3_RS04375 (ACGHX3_04375) pilU 982882..984012 (+) 1131 WP_213600935.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHX3_RS04380 (ACGHX3_04380) - 984261..984641 (-) 381 WP_313928662.1 hypothetical protein -
  ACGHX3_RS04385 (ACGHX3_04385) - 984939..985478 (+) 540 WP_313928661.1 hypothetical protein -
  ACGHX3_RS04390 (ACGHX3_04390) - 985600..985986 (+) 387 WP_162339158.1 glutaredoxin domain-containing protein -
  ACGHX3_RS04395 (ACGHX3_04395) - 986034..987032 (-) 999 WP_394696528.1 alpha/beta hydrolase family protein -
  ACGHX3_RS04400 (ACGHX3_04400) - 987082..987303 (-) 222 WP_394696529.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38604.36 Da        Isoelectric Point: 6.7273

>NTDB_id=1062408 ACGHX3_RS04370 WP_162339156.1 981706..982743(+) (pilT) [Pseudoxanthomonas japonensis strain LY0623]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPLFRELIQQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKIGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQSGMMTLDQHL
QDLVKRSLITRQQAKEYAKEKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1062408 ACGHX3_RS04370 WP_162339156.1 981706..982743(+) (pilT) [Pseudoxanthomonas japonensis strain LY0623]
ATGGATATCGCCGAACTGTTGGCGTTCTCGGTAAAGAACAAAGCGTCCGACCTCCACCTCTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGCATCAACATCCCGGCGCTCGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAAGAGTTCCTCGAGGTCGACTTCTCGTTCGAGATCCCGTCGCTGGCACGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGCTGTTCCGCGAGCTGATCCAACAGCCGCAGGGGTTGATCCTGGTGACCGGCCCGACCGGTT
CCGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTCAGCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCATCGCGACACGCACGGCTTCAACGA
AGCGCTGCGTTCGGCGCTGCGCGAAGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGCCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTCCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGATCGGCGGCGGCCGTACCGCGGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGAGCGGCATGATGACGCTGGACCAGCACCTG
CAGGACCTGGTGAAGCGCAGCCTGATCACGCGCCAGCAGGCCAAGGAATACGCTAAGGAAAAGCGTCTCTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.171

98.261

0.768
  pilT Acinetobacter baylyi ADP1

76.453

99.71

0.762
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.358

97.101

0.664
  pilT Vibrio cholerae strain A1552

68.358

97.101

0.664
  pilT Neisseria meningitidis 8013

64.706

98.551

0.638
  pilT Neisseria gonorrhoeae MS11

64.412

98.551

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.298

98.261

0.406
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Vibrio cholerae strain A1552

40.896

97.101

0.397

Multiple sequence alignment