Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGHX3_RS04375 Genome accession   NZ_CP171632
Coordinates   982882..984012 (+) Length   376 a.a.
NCBI ID   WP_213600935.1    Uniprot ID   -
Organism   Pseudoxanthomonas japonensis strain LY0623     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 977882..989012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHX3_RS04345 (ACGHX3_04345) - 978038..978832 (+) 795 WP_394697240.1 cobalamin-binding protein -
  ACGHX3_RS04350 (ACGHX3_04350) - 978994..979446 (-) 453 WP_394696524.1 DUF4426 domain-containing protein -
  ACGHX3_RS04355 (ACGHX3_04355) proC 979491..980330 (-) 840 WP_394696525.1 pyrroline-5-carboxylate reductase -
  ACGHX3_RS04360 (ACGHX3_04360) - 980339..980914 (-) 576 WP_394696526.1 hypothetical protein -
  ACGHX3_RS04365 (ACGHX3_04365) - 980935..981612 (-) 678 WP_394696527.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHX3_RS04370 (ACGHX3_04370) pilT 981706..982743 (+) 1038 WP_162339156.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHX3_RS04375 (ACGHX3_04375) pilU 982882..984012 (+) 1131 WP_213600935.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHX3_RS04380 (ACGHX3_04380) - 984261..984641 (-) 381 WP_313928662.1 hypothetical protein -
  ACGHX3_RS04385 (ACGHX3_04385) - 984939..985478 (+) 540 WP_313928661.1 hypothetical protein -
  ACGHX3_RS04390 (ACGHX3_04390) - 985600..985986 (+) 387 WP_162339158.1 glutaredoxin domain-containing protein -
  ACGHX3_RS04395 (ACGHX3_04395) - 986034..987032 (-) 999 WP_394696528.1 alpha/beta hydrolase family protein -
  ACGHX3_RS04400 (ACGHX3_04400) - 987082..987303 (-) 222 WP_394696529.1 helix-turn-helix domain-containing protein -
  ACGHX3_RS04405 (ACGHX3_04405) - 987305..987817 (-) 513 WP_394696530.1 DUF2975 domain-containing protein -
  ACGHX3_RS04410 (ACGHX3_04410) - 988083..988595 (+) 513 WP_394696531.1 DUF6622 family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41843.19 Da        Isoelectric Point: 6.8288

>NTDB_id=1062409 ACGHX3_RS04375 WP_213600935.1 982882..984012(+) (pilU) [Pseudoxanthomonas japonensis strain LY0623]
MSTIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKISPITQTPLTSQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTIEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRTQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLVQDYIRDGEVHKLKEVMKESTNLGMKTF
DQALFELYQAGEISYEDALRFADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1062409 ACGHX3_RS04375 WP_213600935.1 982882..984012(+) (pilU) [Pseudoxanthomonas japonensis strain LY0623]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACGTCCGGCAT
GCCGCCATCCATCAAGGTGCACGGCAAGATCTCGCCGATCACGCAGACGCCGCTCACCTCGCAGCAGTCGCGCGACCTGG
TGCTCAACGTCATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGTCGCATCGAGACGCGCATCCC
GACCATCGAGGAACTGAACCTGCCGCCGGTGATCAAGACGCTGGCCATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACG
ATCGAGGATCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTCATCATGATCGGTGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAAACCGGCCATCTGGTGCTGTGTACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCACGCAGCTGTTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGT
GGCGCAGCAGCTGATCCCGACGCCCGACGGCAAGGGCCGCCGGGTGGCGATGGAAATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGGTGCACAAGCTGAAGGAAGTGATGAAGGAATCCACCAACCTCGGCATGAAGACCTTC
GACCAGGCGCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTTCGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCCCGCACGCTGGCGCAGGGGCTGGATGGCGTGGAGATTGCGG
AAGTCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.665

97.606

0.67
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

54.775

94.681

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

93.085

0.399
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367

Multiple sequence alignment