Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGEDC_RS15130 Genome accession   NZ_CP171406
Coordinates   3444032..3445069 (+) Length   345 a.a.
NCBI ID   WP_055026341.1    Uniprot ID   A0A0Q0NVZ9
Organism   Shewanella sp. WPAGA9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3439032..3450069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGEDC_RS15100 rdgB 3439746..3440342 (-) 597 WP_394494166.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  ACGEDC_RS15105 - 3440523..3440957 (-) 435 WP_055026346.1 DUF4426 domain-containing protein -
  ACGEDC_RS15110 yggU 3441156..3441449 (-) 294 WP_394498366.1 DUF167 family protein YggU -
  ACGEDC_RS15115 - 3441554..3442102 (-) 549 WP_065109082.1 YggT family protein -
  ACGEDC_RS15120 proC 3442278..3443096 (-) 819 WP_394494169.1 pyrroline-5-carboxylate reductase -
  ACGEDC_RS15125 - 3443307..3443996 (-) 690 WP_394494171.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGEDC_RS15130 pilT 3444032..3445069 (+) 1038 WP_055026341.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGEDC_RS15135 pilU 3445082..3446194 (+) 1113 WP_055026340.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGEDC_RS15140 - 3446406..3446948 (+) 543 WP_055026339.1 glutathione peroxidase -
  ACGEDC_RS15145 hemH 3447113..3448126 (+) 1014 WP_119968803.1 ferrochelatase -
  ACGEDC_RS15150 ruvX 3448285..3448713 (-) 429 WP_192022365.1 Holliday junction resolvase RuvX -
  ACGEDC_RS15155 - 3448781..3449341 (-) 561 WP_394494175.1 YqgE/AlgH family protein -
  ACGEDC_RS15160 yciH 3449437..3449765 (-) 329 Protein_2939 stress response translation initiation inhibitor YciH -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38259.76 Da        Isoelectric Point: 6.6684

>NTDB_id=1061209 ACGEDC_RS15130 WP_055026341.1 3444032..3445069(+) (pilT) [Shewanella sp. WPAGA9]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQAVHGLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAYNQSRGAGAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINNERHDHILTIED
PIEFVHQNKQCLVNQREVHKHTHSFEAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTVDRI
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLISRDDAMSKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1061209 ACGEDC_RS15130 WP_055026341.1 3444032..3445069(+) (pilT) [Shewanella sp. WPAGA9]
ATGGAAATAACTGAGTTACTTGCCTTTAGTGTAAAACACAATGCCTCGGATCTACACCTTTCAGCAGGTGTTTCGCCGAT
GATTCGAGTTGATGGTGAAGTCAGAAAGATTAATCTACCTGCCTTAGACCACCAAGCAGTTCATGGTCTGGTGTATGACA
TCATGAATGATAAGCAACGTAAGGACTATGAAGAACATCTAGAAATCGATTTCTCATTTGAAGTGCCTAACTTAGCTCGT
TTTCGTGTGAATGCTTATAACCAATCTCGTGGTGCTGGTGCGGTATTTCGTACCATTCCAAGTGATATTTTATCATTAGA
GCAGTTAGGCGCACCTGAAATCTTTAAGAAGATTTCGAGTTTTCCTCGTGGGCTTGTATTGGTTACAGGGCCCACTGGTT
CGGGTAAATCAACCACATTGGCTGCGATGGTCGATTATATTAATAATGAACGTCATGATCACATATTAACCATTGAAGAC
CCAATAGAATTCGTTCACCAAAACAAACAATGTTTGGTCAACCAACGTGAAGTACACAAGCATACCCATAGTTTTGAAGC
GGCGCTTCGAAGTGCACTTCGTGAAGATCCTGATGTCATTCTGGTTGGTGAGATGCGTGACCTTGAAACCATTCGCTTAG
CAATGACAGCAGCAGAAACCGGTCATTTAGTATTTGGTACCTTGCATACAACTTCTGCGGCTAAAACCGTTGACCGTATT
GTGGATGTATTCCCAGCGGGTGAGAAAGACATGGTACGTACTATGTTATCTGAATCATTACAGGCGGTAATTTCGCAAAC
CTTGATTAAGAAAGTCGGTGGTGGCCGAGTCGCTGCCCATGAAATTATGATGGGCACACCTGCAATTAGAAACCTAATTC
GTGAAGATAAAGTCGCGCAAATGTACTCGGCTATTCAAACCGGTATGGCGCACGGAATGCAAACACTTGAGCAATGCTTA
CAAAACTTAGTTAACCGTGGCTTAATTAGCCGTGATGATGCGATGTCTAAAAGCTCAAATAAACAAGCTAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q0NVZ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.134

99.42

0.777
  pilT Acinetobacter nosocomialis M2

78.134

99.42

0.777
  pilT Acinetobacter baumannii strain A118

78.134

99.42

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.681

100

0.777
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Acinetobacter baylyi ADP1

79.697

95.652

0.762
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

68.314

99.71

0.681
  pilT Neisseria gonorrhoeae MS11

68.023

99.71

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.263

98.261

0.484
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

41.964

97.391

0.409
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391

Multiple sequence alignment