Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGEDC_RS15135 Genome accession   NZ_CP171406
Coordinates   3445082..3446194 (+) Length   370 a.a.
NCBI ID   WP_055026340.1    Uniprot ID   A0A0Q0J6J6
Organism   Shewanella sp. WPAGA9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3440082..3451194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGEDC_RS15105 - 3440523..3440957 (-) 435 WP_055026346.1 DUF4426 domain-containing protein -
  ACGEDC_RS15110 yggU 3441156..3441449 (-) 294 WP_394498366.1 DUF167 family protein YggU -
  ACGEDC_RS15115 - 3441554..3442102 (-) 549 WP_065109082.1 YggT family protein -
  ACGEDC_RS15120 proC 3442278..3443096 (-) 819 WP_394494169.1 pyrroline-5-carboxylate reductase -
  ACGEDC_RS15125 - 3443307..3443996 (-) 690 WP_394494171.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGEDC_RS15130 pilT 3444032..3445069 (+) 1038 WP_055026341.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGEDC_RS15135 pilU 3445082..3446194 (+) 1113 WP_055026340.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGEDC_RS15140 - 3446406..3446948 (+) 543 WP_055026339.1 glutathione peroxidase -
  ACGEDC_RS15145 hemH 3447113..3448126 (+) 1014 WP_119968803.1 ferrochelatase -
  ACGEDC_RS15150 ruvX 3448285..3448713 (-) 429 WP_192022365.1 Holliday junction resolvase RuvX -
  ACGEDC_RS15155 - 3448781..3449341 (-) 561 WP_394494175.1 YqgE/AlgH family protein -
  ACGEDC_RS15160 yciH 3449437..3449765 (-) 329 Protein_2939 stress response translation initiation inhibitor YciH -
  ACGEDC_RS15165 - 3450050..3450610 (+) 561 WP_055026334.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41296.24 Da        Isoelectric Point: 5.9381

>NTDB_id=1061210 ACGEDC_RS15135 WP_055026340.1 3445082..3446194(+) (pilU) [Shewanella sp. WPAGA9]
MDVRPFLKVMVDRKASDLFVTAGFPPSAKIDGELRPLSESSFTPEQSLDFVESLMTDVQKQEFHESRECNFAFAAKELGR
FRVSAFWQRESPGCVMRRIETKIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLASLVGYRNSHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPENKHNQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHLLKETMSKSREQGMQTFDQA
LLDLYLEKEISYADALHHADSPNDLRLMIKLQNNESSSSGIMDGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1061210 ACGEDC_RS15135 WP_055026340.1 3445082..3446194(+) (pilU) [Shewanella sp. WPAGA9]
ATGGATGTTCGTCCTTTTTTAAAGGTCATGGTAGACCGTAAAGCATCAGATTTATTTGTTACCGCGGGATTTCCGCCGAG
TGCAAAAATTGATGGTGAATTAAGACCGTTATCAGAAAGCAGTTTTACGCCTGAGCAGTCGTTAGATTTTGTAGAGTCTT
TAATGACAGACGTGCAAAAACAAGAGTTTCATGAATCTCGTGAATGTAACTTTGCTTTTGCGGCTAAAGAATTAGGCCGT
TTTCGTGTTAGTGCTTTTTGGCAACGTGAATCACCTGGTTGTGTGATGCGCCGTATTGAAACAAAAATCCCAGAAGTTGA
AGATTTAAAGCTACCGCCTATTTTAAAAGATTTGGTCATGAGTAAACGTGGTCTGATTATTATGGTCGGTGGTACCGGTA
CTGGTAAATCAACCTCGTTGGCTTCGCTTGTTGGTTATCGTAATTCACATGCTCGAGGTCATATTTTAACGATTGAAGAC
CCAGTGGAATTTGTGCATGACCATCGCAAAAGCATTATTACTCAGCGAGAAGTCGGTATAGACACTGACTCTTTTGATGC
CGCGCTTAAAAGCTCACTTCGTCAGGCGCCAGATGTTATTTTGATTGGTGAAATTCGAACTCAAGAAACCATGGAGTTTG
CGCTGTCATTTGCTGAAACGGGTCACTTATGCATGGCAACACTCCATGCTAACAATGCTAACCAAGCGTTAGACCGTATT
ATGCATTTAGTCCCTGAAAACAAGCATAACCAACTCTTGTTCGATTTGTCGTTAAACTTACGTGGCATTGTCGCTCAACA
GCTTGTGCCTAAATCAGATGGCTCTGGGCGTCGCGCTGCTATTGAAGTGTTAATCAACACTCCTCGTGTGGCGAGTTTGA
TTGCAAAAAATGAGCTGCATTTACTCAAAGAAACCATGAGTAAGTCACGAGAGCAGGGCATGCAGACTTTTGACCAAGCG
TTATTGGATTTATACTTGGAAAAAGAAATCAGCTATGCAGATGCGCTGCATCATGCTGATTCTCCAAACGATTTACGCCT
AATGATAAAACTGCAAAATAATGAGAGTAGCAGTTCTGGCATTATGGATGGTGTCACGTTAGACATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q0J6J6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.838

100

0.6
  pilU Acinetobacter baylyi ADP1

61.364

95.135

0.584
  pilU Vibrio cholerae strain A1552

56.486

100

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.947

86.216

0.37
  pilT Vibrio cholerae strain A1552

42.947

86.216

0.37
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362

Multiple sequence alignment