Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGTJS_RS08900 Genome accession   NZ_CP171363
Coordinates   1796655..1797704 (-) Length   349 a.a.
NCBI ID   WP_315043068.1    Uniprot ID   -
Organism   Faucicola mancuniensis strain GVCNT2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1791655..1802704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGTJS_RS08885 (ACGTJS_08885) - 1792822..1794606 (-) 1785 WP_410471800.1 EAL domain-containing protein -
  ACGTJS_RS08890 (ACGTJS_08890) - 1794593..1795354 (-) 762 WP_410471801.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACGTJS_RS08895 (ACGTJS_08895) pilU 1795410..1796561 (-) 1152 WP_410471802.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGTJS_RS08900 (ACGTJS_08900) pilT 1796655..1797704 (-) 1050 WP_315043068.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGTJS_RS08905 (ACGTJS_08905) - 1797905..1798579 (+) 675 WP_410471803.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGTJS_RS08910 (ACGTJS_08910) - 1798635..1798820 (+) 186 WP_410471804.1 hypothetical protein -
  ACGTJS_RS08915 (ACGTJS_08915) - 1798817..1799266 (+) 450 WP_410471805.1 helix-turn-helix transcriptional regulator -
  ACGTJS_RS08920 (ACGTJS_08920) - 1799269..1800153 (-) 885 WP_410471806.1 hypothetical protein -
  ACGTJS_RS08925 (ACGTJS_08925) - 1800570..1801079 (+) 510 WP_410471807.1 C40 family peptidase -
  ACGTJS_RS08930 (ACGTJS_08930) - 1801239..1802612 (+) 1374 WP_410471808.1 GGDEF domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38861.63 Da        Isoelectric Point: 7.0437

>NTDB_id=1060923 ACGTJS_RS08900 WP_315043068.1 1796655..1797704(-) (pilT) [Faucicola mancuniensis strain GVCNT2]
MSKKLSIDDLLRFSHKNKASDLHISAGLPPMIRVDGEITKINMPPLDHQTVHGLIYDIMNDKQRADYEEFLEADFSFEIP
EVARFRVNAFNQNRGAGAVFRTIPSEVLTMEQLGMGEVFKKISSFKRGVVLVTGPTGSGKSTTLAAMINYINENRKEHIL
TIEDPIEFVHQSKKSLINQREVHRDTLSFDAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKT
IDRVVDVFPAAEKDMIRAMLSESLQAVVSQALLKRQTGGRVAVHEIMLGTPAIRNLIRENKVAQMYSAIQTSAGEGMTTL
DQSLREWLNKGVISKESARAYAKQPEAFM

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=1060923 ACGTJS_RS08900 WP_315043068.1 1796655..1797704(-) (pilT) [Faucicola mancuniensis strain GVCNT2]
ATGTCAAAAAAACTCAGTATTGACGACTTATTACGCTTTTCGCACAAAAATAAAGCATCGGATTTGCACATTTCGGCAGG
TTTGCCACCGATGATTCGTGTGGACGGTGAAATCACCAAAATCAATATGCCACCGCTAGACCACCAAACGGTGCATGGTT
TGATTTATGATATTATGAATGATAAACAACGTGCTGATTATGAAGAGTTTTTGGAAGCGGACTTTTCGTTTGAAATTCCT
GAAGTGGCACGTTTTCGTGTCAATGCCTTTAACCAAAACCGTGGTGCAGGTGCGGTATTTCGTACCATTCCATCAGAAGT
TTTGACGATGGAACAGCTTGGCATGGGTGAAGTGTTTAAAAAAATTTCTAGCTTTAAGCGTGGTGTTGTGTTGGTTACAG
GACCGACAGGTTCGGGCAAGTCTACCACTCTTGCGGCGATGATTAACTATATTAATGAAAATCGTAAAGAGCATATTTTG
ACCATTGAAGACCCGATTGAGTTCGTTCATCAATCCAAAAAATCACTGATTAACCAGCGTGAAGTCCACCGTGATACCTT
AAGTTTTGACGCAGCCTTGCGTTCGGCACTGCGTGAAGACCCTGACGTGATTTTGGTGGGTGAGATGCGAGATTTGGAGA
CCATTCGTCTTGCCTTGACCGCCGCTGAAACAGGACACTTGGTATTTGGTACATTGCACACCACATCTGCTGCCAAAACC
ATTGACCGTGTGGTGGACGTATTCCCTGCTGCTGAAAAAGATATGATTCGTGCGATGTTATCAGAATCATTGCAAGCCGT
TGTTTCACAAGCCTTGTTAAAACGTCAAACAGGCGGTCGTGTGGCGGTGCATGAAATCATGCTTGGCACGCCTGCGATTC
GTAACTTGATTCGTGAAAATAAAGTCGCACAAATGTACTCGGCAATCCAAACCAGTGCTGGCGAAGGTATGACAACGCTT
GACCAAAGTTTGCGTGAATGGCTCAACAAAGGCGTGATTAGCAAAGAATCCGCTCGTGCGTATGCAAAACAGCCTGAAGC
CTTTATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.841

98.854

0.779
  pilT Acinetobacter baumannii D1279779

78.841

98.854

0.779
  pilT Acinetobacter baumannii strain A118

78.841

98.854

0.779
  pilT Pseudomonas aeruginosa PAK

77.035

98.567

0.759
  pilT Pseudomonas stutzeri DSM 10701

77.035

98.567

0.759
  pilT Acinetobacter baylyi ADP1

75.872

98.567

0.748
  pilT Legionella pneumophila strain Lp02

72.093

98.567

0.711
  pilT Legionella pneumophila strain ERS1305867

72.093

98.567

0.711
  pilT Neisseria meningitidis 8013

66.087

98.854

0.653
  pilT Neisseria gonorrhoeae MS11

65.797

98.854

0.65
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.262

96.275

0.648
  pilT Vibrio cholerae strain A1552

67.262

96.275

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.576

100

0.516
  pilU Pseudomonas stutzeri DSM 10701

41.593

97.135

0.404
  pilU Acinetobacter baylyi ADP1

40.233

98.281

0.395
  pilU Vibrio cholerae strain A1552

41.796

92.55

0.387

Multiple sequence alignment