Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGTJS_RS08895 Genome accession   NZ_CP171363
Coordinates   1795410..1796561 (-) Length   383 a.a.
NCBI ID   WP_410471802.1    Uniprot ID   -
Organism   Faucicola mancuniensis strain GVCNT2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1790410..1801561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGTJS_RS08880 (ACGTJS_08880) - 1790933..1792729 (-) 1797 WP_410471799.1 acyl-CoA dehydrogenase C-terminal domain-containing protein -
  ACGTJS_RS08885 (ACGTJS_08885) - 1792822..1794606 (-) 1785 WP_410471800.1 EAL domain-containing protein -
  ACGTJS_RS08890 (ACGTJS_08890) - 1794593..1795354 (-) 762 WP_410471801.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACGTJS_RS08895 (ACGTJS_08895) pilU 1795410..1796561 (-) 1152 WP_410471802.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGTJS_RS08900 (ACGTJS_08900) pilT 1796655..1797704 (-) 1050 WP_315043068.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGTJS_RS08905 (ACGTJS_08905) - 1797905..1798579 (+) 675 WP_410471803.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGTJS_RS08910 (ACGTJS_08910) - 1798635..1798820 (+) 186 WP_410471804.1 hypothetical protein -
  ACGTJS_RS08915 (ACGTJS_08915) - 1798817..1799266 (+) 450 WP_410471805.1 helix-turn-helix transcriptional regulator -
  ACGTJS_RS08920 (ACGTJS_08920) - 1799269..1800153 (-) 885 WP_410471806.1 hypothetical protein -
  ACGTJS_RS08925 (ACGTJS_08925) - 1800570..1801079 (+) 510 WP_410471807.1 C40 family peptidase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42983.30 Da        Isoelectric Point: 5.6401

>NTDB_id=1060922 ACGTJS_RS08895 WP_410471802.1 1795410..1796561(-) (pilU) [Faucicola mancuniensis strain GVCNT2]
MDFDKLLQLMVQKNGSDLFITADVPPSMKVNGVIMPVTKTPLTSEQSMAIVKSVMTPAQIKEFDETKECQFAISDKNKTA
RFRVSAFIQKDAAGMILRKIETKIPTVEQLNLPPILNEVAMKKRGIVLLVGATGTGKSTTLAAMMGYRNENSRGHIITIE
DPIEYVHEHKGCIVTQREVGIDTMSFEAGLKNTLRQAPDVILIGEIRTRETMDYAVQYAETGHLVFATLHANNANQAIDR
IIHFFEAEQHEQILMDLSLNLRAIIAQQLIPTPDGKSRRAAIEILLNTQLIGDYIRKGEIHEIKPVMKRSREVGMQTFDQ
ALFDLYENGEITYQDALKSADSPNDLRLMIKLHSKHAAQVDDEDSNFSLQADEDYTDKGNFFS

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=1060922 ACGTJS_RS08895 WP_410471802.1 1795410..1796561(-) (pilU) [Faucicola mancuniensis strain GVCNT2]
ATGGATTTTGATAAATTATTACAATTAATGGTACAAAAAAACGGTTCTGACTTGTTTATCACCGCCGATGTACCGCCTTC
AATGAAGGTCAATGGCGTGATTATGCCTGTCACCAAAACCCCTTTAACCTCTGAGCAATCTATGGCGATTGTCAAAAGTG
TGATGACACCTGCTCAAATTAAAGAATTTGACGAAACCAAAGAATGTCAATTTGCGATTAGTGATAAAAATAAAACCGCA
CGTTTTCGTGTGTCAGCCTTTATCCAAAAAGACGCTGCGGGTATGATTTTGCGTAAAATTGAAACCAAAATTCCAACGGT
TGAACAGCTTAATTTGCCACCGATTTTGAACGAAGTGGCAATGAAAAAACGTGGTATCGTGCTACTCGTAGGTGCGACTG
GTACTGGTAAATCCACGACACTGGCTGCGATGATGGGTTATCGTAATGAAAACTCTCGTGGACATATCATCACCATTGAA
GACCCGATTGAATACGTACACGAACACAAAGGCTGTATCGTTACCCAACGAGAAGTTGGCATTGATACTATGTCGTTTGA
AGCGGGGCTAAAAAACACCCTGCGTCAAGCCCCAGACGTGATTTTGATTGGTGAGATTCGTACGCGTGAAACGATGGACT
ATGCGGTACAATACGCTGAAACAGGTCACTTGGTATTTGCTACCCTGCACGCCAACAACGCCAACCAAGCGATTGACCGT
ATTATTCACTTTTTTGAAGCGGAACAACATGAACAAATTTTGATGGATTTATCATTGAACTTGCGTGCTATTATCGCTCA
GCAGTTGATTCCAACGCCTGATGGTAAAAGTCGCCGTGCCGCCATTGAGATTTTGTTAAATACTCAATTAATCGGTGATT
ATATTCGTAAAGGCGAAATCCATGAGATTAAACCTGTAATGAAACGTTCTCGTGAAGTCGGTATGCAGACCTTTGACCAA
GCCTTGTTTGACCTATACGAAAACGGCGAAATCACTTATCAAGATGCGTTAAAATCTGCCGACAGTCCGAATGACTTACG
TCTGATGATTAAGCTACACAGTAAGCACGCCGCCCAAGTTGATGATGAAGATTCTAACTTTTCATTACAAGCTGATGAAG
ATTATACCGACAAAGGCAATTTTTTCTCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.296

92.689

0.642
  pilU Acinetobacter baylyi ADP1

65.11

95.039

0.619
  pilU Vibrio cholerae strain A1552

56.125

91.645

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

90.339

0.384
  pilT Legionella pneumophila strain Lp02

42.941

88.773

0.381
  pilT Legionella pneumophila strain ERS1305867

42.941

88.773

0.381
  pilT Acinetobacter baumannii D1279779

40.816

89.556

0.366
  pilT Acinetobacter nosocomialis M2

40.816

89.556

0.366
  pilT Acinetobacter baumannii strain A118

40.816

89.556

0.366
  pilT Pseudomonas aeruginosa PAK

40.462

90.339

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.194

87.467

0.36
  pilT Vibrio cholerae strain A1552

41.194

87.467

0.36

Multiple sequence alignment