Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACE1MM_RS02505 Genome accession   NZ_CP171355
Coordinates   524138..525181 (+) Length   347 a.a.
NCBI ID   WP_045787652.1    Uniprot ID   A0A0D5ASY1
Organism   Ralstonia mannitolilytica strain SY001     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 519138..530181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1MM_RS02485 (ACE1MM_02485) ubiA 521263..522138 (+) 876 WP_062737985.1 4-hydroxybenzoate octaprenyltransferase -
  ACE1MM_RS02490 (ACE1MM_02490) - 522135..522410 (-) 276 WP_062737935.1 hypothetical protein -
  ACE1MM_RS02495 (ACE1MM_02495) proC 522443..523267 (-) 825 WP_045787654.1 pyrroline-5-carboxylate reductase -
  ACE1MM_RS02500 (ACE1MM_02500) - 523298..524014 (-) 717 WP_062737934.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACE1MM_RS02505 (ACE1MM_02505) pilT 524138..525181 (+) 1044 WP_045787652.1 type IV pilus twitching motility protein PilT Machinery gene
  ACE1MM_RS02510 (ACE1MM_02510) pilU 525235..526374 (+) 1140 WP_045787651.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACE1MM_RS02515 (ACE1MM_02515) - 526393..526890 (+) 498 WP_062737933.1 glutathione peroxidase -
  ACE1MM_RS02520 (ACE1MM_02520) - 526907..527605 (-) 699 WP_045787649.1 YafY family protein -
  ACE1MM_RS02525 (ACE1MM_02525) - 527649..528128 (-) 480 WP_045787648.1 cupin domain-containing protein -
  ACE1MM_RS02530 (ACE1MM_02530) - 528192..528587 (-) 396 WP_062737932.1 VOC family protein -
  ACE1MM_RS02535 (ACE1MM_02535) - 528759..529490 (-) 732 WP_045787646.1 LrgB family protein -
  ACE1MM_RS02540 (ACE1MM_02540) - 529487..529864 (-) 378 WP_045787645.1 CidA/LrgA family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38264.01 Da        Isoelectric Point: 6.7828

>NTDB_id=1060832 ACE1MM_RS02505 WP_045787652.1 524138..525181(+) (pilT) [Ralstonia mannitolilytica strain SY001]
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKVYEENLEVDFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPPVFAELAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVED
PIEFVHESKKSLINQRELGPHTLSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPSEEKDMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMITTPAIRHLIRENKISQMYSMMQTSSGMGMQTLDQC
LADLIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1060832 ACE1MM_RS02505 WP_045787652.1 524138..525181(+) (pilT) [Ralstonia mannitolilytica strain SY001]
ATGGACATCGCGCAGCTTCTGGCCTTCTCGGCCAAGAACAAGGCGTCGGACCTGCATCTGTCGGCCGGCCTGCCGCCGAT
GATCCGGATCCACGGCGACATGCGCCGGATCAATGTGCCGCCGCTCACGCACCAGGATGTGCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGGTCTACGAAGAAAACCTGGAAGTCGACTTCTCGTTTGAGATTCCGGGCCTGTCCCGC
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCCTCGGCCGTGTTCCGGACGATTCCGTCGAAGGTGCTCTCGCTGGA
CGACCTGAAGGCGCCGCCCGTGTTTGCCGAGCTGGCGATGAAGCCGCGCGGCCTGGTGCTCGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCCGCGATGGTGAACCACCGCAACGAAAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCGAAGAAGAGCCTGATCAACCAGCGCGAACTCGGGCCGCACACGCTGTCGTTCGCCAA
TGCACTGAAGTCGGCGCTGCGTGAAGACCCGGACGTCATCCTCGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACGGCCGCTGAAACCGGCCACTTGGTGTTCGCCACGCTGCACACGAGTTCTGCGGCCAAGACGATCGACCGGGTG
GTCGACGTGTTCCCGTCGGAAGAGAAAGACATGGTGCGGACCATGCTTTCTGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCGGGGCGGATTGCGGCGCACGAGATCATGATCACGACGCCGGCCATCCGCCACCTGA
TCCGTGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACCAGCAGCGGCATGGGCATGCAGACGCTCGACCAGTGC
CTGGCCGACCTCATCAAGCGCTCGGCGATCAACTACGCGGATGCGCGCGCCATCGCCAAGAACCCCGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D5ASY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

71.014

99.424

0.706
  pilT Acinetobacter nosocomialis M2

71.014

99.424

0.706
  pilT Acinetobacter baumannii D1279779

71.014

99.424

0.706
  pilT Acinetobacter baumannii strain A118

71.014

99.424

0.706
  pilT Pseudomonas stutzeri DSM 10701

70.725

99.424

0.703
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Neisseria gonorrhoeae MS11

68.497

99.712

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Legionella pneumophila strain Lp02

67.826

99.424

0.674
  pilT Legionella pneumophila strain ERS1305867

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.118

0.64
  pilT Vibrio cholerae strain A1552

65.875

97.118

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.289

99.712

0.501
  pilU Acinetobacter baylyi ADP1

43.895

99.135

0.435
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403

Multiple sequence alignment