Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACE1MM_RS02510 Genome accession   NZ_CP171355
Coordinates   525235..526374 (+) Length   379 a.a.
NCBI ID   WP_045787651.1    Uniprot ID   A0AAJ5D5Q9
Organism   Ralstonia mannitolilytica strain SY001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 520235..531374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1MM_RS02485 (ACE1MM_02485) ubiA 521263..522138 (+) 876 WP_062737985.1 4-hydroxybenzoate octaprenyltransferase -
  ACE1MM_RS02490 (ACE1MM_02490) - 522135..522410 (-) 276 WP_062737935.1 hypothetical protein -
  ACE1MM_RS02495 (ACE1MM_02495) proC 522443..523267 (-) 825 WP_045787654.1 pyrroline-5-carboxylate reductase -
  ACE1MM_RS02500 (ACE1MM_02500) - 523298..524014 (-) 717 WP_062737934.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACE1MM_RS02505 (ACE1MM_02505) pilT 524138..525181 (+) 1044 WP_045787652.1 type IV pilus twitching motility protein PilT Machinery gene
  ACE1MM_RS02510 (ACE1MM_02510) pilU 525235..526374 (+) 1140 WP_045787651.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACE1MM_RS02515 (ACE1MM_02515) - 526393..526890 (+) 498 WP_062737933.1 glutathione peroxidase -
  ACE1MM_RS02520 (ACE1MM_02520) - 526907..527605 (-) 699 WP_045787649.1 YafY family protein -
  ACE1MM_RS02525 (ACE1MM_02525) - 527649..528128 (-) 480 WP_045787648.1 cupin domain-containing protein -
  ACE1MM_RS02530 (ACE1MM_02530) - 528192..528587 (-) 396 WP_062737932.1 VOC family protein -
  ACE1MM_RS02535 (ACE1MM_02535) - 528759..529490 (-) 732 WP_045787646.1 LrgB family protein -
  ACE1MM_RS02540 (ACE1MM_02540) - 529487..529864 (-) 378 WP_045787645.1 CidA/LrgA family protein -
  ACE1MM_RS02545 (ACE1MM_02545) glcF 529916..531160 (-) 1245 WP_062737931.1 glycolate oxidase subunit GlcF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42241.65 Da        Isoelectric Point: 6.8849

>NTDB_id=1060833 ACE1MM_RS02510 WP_045787651.1 525235..526374(+) (pilU) [Ralstonia mannitolilytica strain SY001]
MLDREAATKYIHELLQLMVNSRGSDLFITSDFPPAIKVDGKVTPISQQPLNATQAMSLVKAIMNEKQLREYEQTLECNFA
ITAPGAGRFRVSAFVQQGRAGMVLRTINTKIPSLDELDLPPILNDIVMSKRGLVIVVGATGSGKSTSLAAMVGHRNANSY
GHIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRKGKKGRVPAVEIMLATPLVQDLIFKGEVHELKEVMKKSREQ
GMISFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRAGQTDLAAGTEHLNVV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1060833 ACE1MM_RS02510 WP_045787651.1 525235..526374(+) (pilU) [Ralstonia mannitolilytica strain SY001]
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCCTGCAGTTGATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACGTCGGATTTCCCGCCCGCCATCAAGGTGGACGGCAAGGTCACGCCGATCTCGCAGCAGCCGTTGAACGCCACGC
AGGCCATGAGCCTGGTCAAGGCGATCATGAACGAGAAGCAGCTGCGCGAGTACGAACAGACCCTTGAGTGCAACTTCGCC
ATCACGGCCCCGGGTGCCGGGCGCTTTCGCGTGTCGGCCTTCGTGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CAACACGAAGATCCCCTCGCTGGACGAGCTTGACCTGCCGCCGATCCTCAACGACATCGTGATGAGCAAGCGCGGCCTGG
TGATCGTGGTGGGCGCCACCGGCTCGGGCAAGTCGACCTCGCTGGCGGCCATGGTGGGGCATCGCAACGCCAATTCGTAC
GGCCATATCATCACGATCGAAGACCCGGTCGAATACGTGCACGCGCACCAGAACTGTGTGGTCACGCAGCGCGAGGTCGG
CGTGGACACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGATGTGATCCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAATACGCCATCCAGTATGCGGAAACCGGCCACCTGTGCCTCGCCACGCTGCACGCCAACAGC
TCGAACCAGGCCATCGACCGCATCATCAACTTCTTTCCGGAAGAGAAGCGCCAGCAGTTGCTGATCGACCTCTCGCTGAA
CCTGCGCGCGATGATTGCGCAGCGCCTGCTGCCGCGCAAAGGCAAGAAGGGGCGCGTGCCGGCCGTGGAGATCATGCTGG
CCACGCCGCTGGTGCAGGACCTCATCTTCAAGGGCGAGGTGCACGAGCTGAAGGAGGTCATGAAGAAATCGCGCGAGCAG
GGCATGATTTCCTTCGACCAGGCGCTGTTCGAGCTGTACGAGGCGGACAAGATCACCTACGAAGACGCCCTGCGCAACGC
CGACTCGCTCAACGACCTGCGCCTGCAGATCAAGCTGCACAGCAAGCGCGCCGGGCAGACCGACCTGGCGGCCGGCACCG
AACATCTGAACGTGGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.674

93.931

0.57
  pilU Acinetobacter baylyi ADP1

55.714

92.348

0.515
  pilU Vibrio cholerae strain A1552

54.023

91.821

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.027

88.918

0.383
  pilT Acinetobacter baylyi ADP1

44.41

84.96

0.377
  pilT Legionella pneumophila strain Lp02

42.943

87.863

0.377
  pilT Legionella pneumophila strain ERS1305867

42.943

87.863

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.11

83.641

0.377
  pilT Vibrio cholerae strain A1552

45.11

83.641

0.377
  pilT Acinetobacter nosocomialis M2

44.201

84.169

0.372
  pilT Acinetobacter baumannii D1279779

44.201

84.169

0.372
  pilT Acinetobacter baumannii strain A118

44.201

84.169

0.372
  pilT Pseudomonas aeruginosa PAK

41.84

88.918

0.372
  pilT Pseudomonas stutzeri DSM 10701

41.84

88.918

0.372

Multiple sequence alignment