Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACF3M1_RS04510 Genome accession   NZ_CP171131
Coordinates   996036..997073 (+) Length   345 a.a.
NCBI ID   WP_394004292.1    Uniprot ID   -
Organism   Luteimonas sp. WGS1318     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 991036..1002073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3M1_RS04480 (ACF3M1_04480) - 991146..992219 (+) 1074 WP_394004280.1 OmpA family protein -
  ACF3M1_RS04485 (ACF3M1_04485) - 992339..992941 (+) 603 WP_394004282.1 hypothetical protein -
  ACF3M1_RS04490 (ACF3M1_04490) - 993036..993845 (+) 810 WP_394004284.1 pseudouridine synthase -
  ACF3M1_RS04495 (ACF3M1_04495) - 993823..994308 (-) 486 WP_394004286.1 DUF4426 domain-containing protein -
  ACF3M1_RS04500 (ACF3M1_04500) proC 994301..995152 (-) 852 WP_394004288.1 pyrroline-5-carboxylate reductase -
  ACF3M1_RS04505 (ACF3M1_04505) - 995212..995904 (-) 693 WP_394004290.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACF3M1_RS04510 (ACF3M1_04510) pilT 996036..997073 (+) 1038 WP_394004292.1 type IV pilus twitching motility protein PilT Machinery gene
  ACF3M1_RS04515 (ACF3M1_04515) pilU 997205..998335 (+) 1131 WP_394004294.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACF3M1_RS04520 (ACF3M1_04520) - 998526..999152 (+) 627 WP_394004296.1 superoxide dismutase -
  ACF3M1_RS04525 (ACF3M1_04525) - 999218..1000282 (-) 1065 WP_394004298.1 hypothetical protein -
  ACF3M1_RS04530 (ACF3M1_04530) - 1000282..1000818 (-) 537 WP_394004300.1 DNA-3-methyladenine glycosylase I -
  ACF3M1_RS04535 (ACF3M1_04535) - 1000926..1001381 (+) 456 WP_394004302.1 peptidylprolyl isomerase -
  ACF3M1_RS04540 (ACF3M1_04540) - 1001457..1002011 (+) 555 WP_394004304.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38602.33 Da        Isoelectric Point: 6.7271

>NTDB_id=1060275 ACF3M1_RS04510 WP_394004292.1 996036..997073(+) (pilT) [Luteimonas sp. WGS1318]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHALIYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLNCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPSGEKPMVRSMLSESLRAVISQSLLKKVGGGRTAAWEIMVGVPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQHL
QDLVKRGLITRPAAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1060275 ACF3M1_RS04510 WP_394004292.1 996036..997073(+) (pilT) [Luteimonas sp. WGS1318]
ATGGATATCGCCGAACTGTTGGCGTTCTCGGTCAAGAACAAGGCGTCGGACCTGCATCTGTCGGCAGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGTCGCATCAACATCCCCGCGCTCGAGCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGTCGCGACTTCGAAGAATTCCTCGAGGTCGACTTCTCGTTCGAGATCCCGGGCCTGGCCCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCGTCGGAAGTGCTGACGCTCGA
GGATCTGAACTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGTCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACCCTCGCGGCGATGATCGACCACATCAACAAGAACGAATACGCGCACATCCTCTCGGTCGAAGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACCCATGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGACCCCGACTACATTCTGGTCGGCGAGTTGCGCGACCTCGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTGTTCGGCACGCTGCACACATCCAGCGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGTCGGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCGGAAAGCTTGCGCGCGGTGATCTCGCAGTC
GCTGCTGAAGAAGGTCGGCGGCGGCCGGACCGCGGCCTGGGAGATCATGGTCGGCGTGCCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGCAGACGCTCGACCAGCACCTG
CAGGATCTGGTCAAGCGCGGCCTGATCACGCGCCCGGCCGCGAAGGAATACGCGAAGGACAAGCGTCTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Neisseria meningitidis 8013

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.942

0.394
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391
  pilU Vibrio cholerae strain A1552

40.923

94.203

0.386

Multiple sequence alignment