Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACF3M1_RS04515 Genome accession   NZ_CP171131
Coordinates   997205..998335 (+) Length   376 a.a.
NCBI ID   WP_394004294.1    Uniprot ID   -
Organism   Luteimonas sp. WGS1318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 992205..1003335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3M1_RS04485 (ACF3M1_04485) - 992339..992941 (+) 603 WP_394004282.1 hypothetical protein -
  ACF3M1_RS04490 (ACF3M1_04490) - 993036..993845 (+) 810 WP_394004284.1 pseudouridine synthase -
  ACF3M1_RS04495 (ACF3M1_04495) - 993823..994308 (-) 486 WP_394004286.1 DUF4426 domain-containing protein -
  ACF3M1_RS04500 (ACF3M1_04500) proC 994301..995152 (-) 852 WP_394004288.1 pyrroline-5-carboxylate reductase -
  ACF3M1_RS04505 (ACF3M1_04505) - 995212..995904 (-) 693 WP_394004290.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACF3M1_RS04510 (ACF3M1_04510) pilT 996036..997073 (+) 1038 WP_394004292.1 type IV pilus twitching motility protein PilT Machinery gene
  ACF3M1_RS04515 (ACF3M1_04515) pilU 997205..998335 (+) 1131 WP_394004294.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACF3M1_RS04520 (ACF3M1_04520) - 998526..999152 (+) 627 WP_394004296.1 superoxide dismutase -
  ACF3M1_RS04525 (ACF3M1_04525) - 999218..1000282 (-) 1065 WP_394004298.1 hypothetical protein -
  ACF3M1_RS04530 (ACF3M1_04530) - 1000282..1000818 (-) 537 WP_394004300.1 DNA-3-methyladenine glycosylase I -
  ACF3M1_RS04535 (ACF3M1_04535) - 1000926..1001381 (+) 456 WP_394004302.1 peptidylprolyl isomerase -
  ACF3M1_RS04540 (ACF3M1_04540) - 1001457..1002011 (+) 555 WP_394004304.1 YqgE/AlgH family protein -
  ACF3M1_RS04545 (ACF3M1_04545) ruvX 1002008..1002484 (+) 477 WP_394004306.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41777.11 Da        Isoelectric Point: 6.8808

>NTDB_id=1060276 ACF3M1_RS04515 WP_394004294.1 997205..998335(+) (pilU) [Luteimonas sp. WGS1318]
MSSIDFTSFLKLMAHQKASDLFITAGMPPSIKVHGKISPITQNPLTPQQARDLVLNVMTPPQREEFEKTHECNFAIGVSG
VGRFRISCFYQRNQVGMVLRRIETHIPTIEELNLPPIIKTLAMTKRGIVIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHAGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGKGRRAAMEIMLGTPLVQDYIREGEIHKLKEVMKESTNLGMKTF
DQALFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1060276 ACF3M1_RS04515 WP_394004294.1 997205..998335(+) (pilU) [Luteimonas sp. WGS1318]
ATGAGCAGCATCGATTTCACCTCGTTTCTCAAACTGATGGCCCATCAAAAGGCCTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATCAAGGTGCACGGCAAGATCTCGCCGATCACCCAGAACCCGCTGACGCCGCAGCAGGCGCGCGATCTGG
TGCTCAACGTGATGACCCCGCCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCGATCGGCGTCTCCGGC
GTCGGCCGCTTCCGCATCTCGTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCACATCCC
GACGATCGAGGAGCTCAACCTCCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCGTGATCTTCGTCGGCG
CGACCGGTACCGGTAAGTCGACGTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCCACCGGCCACATCATCACG
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGCGGGCTGCATCATCACCCAGCGTGAAGTCGGCATCGATACCGACAG
CTGGGACGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCGCATTCGCGGAAACCGGCCATCTGGTGCTGTGCACGCTGCATGCGAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCAACCAGCTGCTGATGGATCTCTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATCCCGACGCCCGACGGCAAGGGCCGGCGCGCGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAAGGCGAGATCCACAAGCTCAAGGAGGTCATGAAGGAATCGACCAACCTGGGCATGAAGACCTTC
GACCAGGCGCTGTTCGAGCTCTACCAGGCCGGCGAGATCTCCTACGAGGACGCGCTGCGTTACGCCGACTCCGCGAACGA
GGTGCGCCTGCGCATCAAGCTCGCGCAGGGCGGCGATGCGCGCACGCTGTCGCAGGGGCTGGACGGGGTCGAGGTCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.392

97.606

0.668
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.695

90.691

0.396
  pilT Pseudomonas aeruginosa PAK

41.791

89.096

0.372
  pilT Legionella pneumophila strain Lp02

41.493

89.096

0.37
  pilT Legionella pneumophila strain ERS1305867

41.493

89.096

0.37
  pilT Acinetobacter nosocomialis M2

41.194

89.096

0.367
  pilT Acinetobacter baumannii D1279779

41.194

89.096

0.367
  pilT Acinetobacter baumannii strain A118

41.194

89.096

0.367

Multiple sequence alignment