Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACF3M1_RS03340 Genome accession   NZ_CP171131
Coordinates   750167..750589 (-) Length   140 a.a.
NCBI ID   WP_394005159.1    Uniprot ID   -
Organism   Luteimonas sp. WGS1318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 745167..755589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3M1_RS03320 (ACF3M1_03320) - 745490..746410 (-) 921 WP_394003915.1 NAD-dependent epimerase/dehydratase family protein -
  ACF3M1_RS03325 (ACF3M1_03325) - 746403..747545 (-) 1143 WP_394003917.1 glycosyltransferase family 4 protein -
  ACF3M1_RS03330 (ACF3M1_03330) - 747645..749555 (-) 1911 WP_394003919.1 hypothetical protein -
  ACF3M1_RS03335 (ACF3M1_03335) pilA2 749635..750057 (-) 423 WP_394003921.1 pilin Machinery gene
  ACF3M1_RS03340 (ACF3M1_03340) pilE 750167..750589 (-) 423 WP_394005159.1 pilin Machinery gene
  ACF3M1_RS03345 (ACF3M1_03345) - 750779..752635 (-) 1857 WP_394003923.1 hypothetical protein -
  ACF3M1_RS03350 (ACF3M1_03350) pilR 752710..754125 (-) 1416 WP_394003925.1 sigma-54-dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14560.57 Da        Isoelectric Point: 8.9613

>NTDB_id=1060270 ACF3M1_RS03340 WP_394005159.1 750167..750589(-) (pilE) [Luteimonas sp. WGS1318]
MLQKGFTLIELMIVVAIIAILAAIALPAYQDYVARSQVSEGMSLSAGAKTAIAEYYANFAAWPATNTAAGLATANSISGK
YVSSTTVGADGIITVAYNQSATSTKIRNNNLTLTPTDRGGSVAWACAGSIDNKYRPSSCR

Nucleotide


Download         Length: 423 bp        

>NTDB_id=1060270 ACF3M1_RS03340 WP_394005159.1 750167..750589(-) (pilE) [Luteimonas sp. WGS1318]
ATGCTGCAGAAGGGCTTTACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGCCATCCTGGCCGCCATCGCGCTGCC
GGCCTACCAGGACTACGTGGCCCGCTCGCAGGTGTCGGAAGGCATGAGCCTGTCGGCTGGAGCGAAGACCGCGATCGCCG
AGTACTACGCTAACTTCGCGGCGTGGCCGGCGACTAACACAGCCGCAGGTCTTGCGACTGCGAACTCGATCAGCGGCAAG
TACGTGTCCAGCACCACCGTCGGCGCCGATGGCATCATTACGGTTGCGTACAACCAGAGCGCGACCAGCACCAAGATCCG
CAATAACAATCTGACGCTGACCCCGACCGACCGCGGCGGCAGCGTGGCCTGGGCCTGCGCCGGCTCGATCGACAACAAGT
ACCGCCCGAGCTCCTGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-26)

Pilin (bacterial filament) (Pilin)

(34-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

46.795

100

0.521
  pilE Neisseria gonorrhoeae MS11

45.86

100

0.514
  pilA2 Legionella pneumophila strain ERS1305867

47.887

100

0.486
  pilA2 Legionella pneumophila str. Paris

47.887

100

0.486
  pilA/pilA1 Eikenella corrodens VA1

43.226

100

0.479
  comP Acinetobacter baylyi ADP1

44.966

100

0.479
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.135

100

0.464
  pilA Ralstonia pseudosolanacearum GMI1000

38.182

100

0.45
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.844

100

0.421
  pilA Vibrio cholerae C6706

38.776

100

0.407
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.776

100

0.407
  pilA Vibrio cholerae strain A1552

38.776

100

0.407
  pilA Acinetobacter baumannii strain A118

40.426

100

0.407
  pilA Pseudomonas aeruginosa PAK

36.913

100

0.393
  pilA Acinetobacter nosocomialis M2

41.406

91.429

0.379

Multiple sequence alignment