Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ACF3M1_RS03335 Genome accession   NZ_CP171131
Coordinates   749635..750057 (-) Length   140 a.a.
NCBI ID   WP_394003921.1    Uniprot ID   -
Organism   Luteimonas sp. WGS1318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 744635..755057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3M1_RS03320 (ACF3M1_03320) - 745490..746410 (-) 921 WP_394003915.1 NAD-dependent epimerase/dehydratase family protein -
  ACF3M1_RS03325 (ACF3M1_03325) - 746403..747545 (-) 1143 WP_394003917.1 glycosyltransferase family 4 protein -
  ACF3M1_RS03330 (ACF3M1_03330) - 747645..749555 (-) 1911 WP_394003919.1 hypothetical protein -
  ACF3M1_RS03335 (ACF3M1_03335) pilA2 749635..750057 (-) 423 WP_394003921.1 pilin Machinery gene
  ACF3M1_RS03340 (ACF3M1_03340) pilE 750167..750589 (-) 423 WP_394005159.1 pilin Machinery gene
  ACF3M1_RS03345 (ACF3M1_03345) - 750779..752635 (-) 1857 WP_394003923.1 hypothetical protein -
  ACF3M1_RS03350 (ACF3M1_03350) pilR 752710..754125 (-) 1416 WP_394003925.1 sigma-54-dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14966.04 Da        Isoelectric Point: 7.2410

>NTDB_id=1060269 ACF3M1_RS03335 WP_394003921.1 749635..750057(-) (pilA2) [Luteimonas sp. WGS1318]
MRSKGFTLIELMIVVAIIAILAAIALPAYQDYVARSQVAEGLVVIGGAKLAVTEYHAHNGAWPDSHAAMELAPAQSISGR
YVSSASVGTDGRITVAYNRSASSEKIRSAELVLVPRTSDQTVSWDCEGTVDPRYRPSSCR

Nucleotide


Download         Length: 423 bp        

>NTDB_id=1060269 ACF3M1_RS03335 WP_394003921.1 749635..750057(-) (pilA2) [Luteimonas sp. WGS1318]
ATGCGCTCGAAGGGATTCACGCTGATCGAACTGATGATCGTGGTGGCGATTATCGCCATCCTGGCTGCTATCGCGCTGCC
CGCCTACCAGGATTATGTTGCCCGGAGCCAGGTCGCCGAGGGGCTTGTGGTCATCGGCGGCGCCAAGCTCGCCGTCACCG
AATACCACGCGCACAACGGCGCGTGGCCGGACAGCCACGCCGCCATGGAGCTGGCACCGGCGCAATCCATCAGTGGACGC
TACGTGTCCTCAGCCTCGGTGGGGACCGATGGACGCATCACGGTCGCCTACAACCGATCGGCCTCGAGCGAAAAGATCCG
GAGCGCCGAGCTGGTCCTGGTGCCCCGCACCTCCGACCAGACCGTTTCCTGGGACTGCGAAGGCACGGTGGATCCCCGCT
ATCGCCCCAGCTCCTGCCGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(34-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

48.252

100

0.493
  pilA2 Legionella pneumophila strain ERS1305867

47.552

100

0.486
  pilE Neisseria gonorrhoeae MS11

40.645

100

0.45
  pilE Neisseria gonorrhoeae strain FA1090

39.61

100

0.436
  comP Acinetobacter baylyi ADP1

41.096

100

0.429
  pilA Ralstonia pseudosolanacearum GMI1000

36.585

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.146

97.857

0.393
  pilA Acinetobacter baumannii strain A118

39.568

99.286

0.393
  pilA Pseudomonas aeruginosa PAK

36.486

100

0.386
  pilA/pilA1 Eikenella corrodens VA1

36.486

100

0.386
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.686

97.857

0.379
  pilA Vibrio cholerae C6706

36.054

100

0.379
  pilA Vibrio cholerae strain A1552

36.054

100

0.379
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.054

100

0.379

Multiple sequence alignment