Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACFCQI_RS12415 Genome accession   NZ_CP170722
Coordinates   2779494..2779937 (-) Length   147 a.a.
NCBI ID   WP_395117126.1    Uniprot ID   A0AB74UKE2
Organism   Rhodanobacter sp. FW102-FHT14D06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2774494..2784937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFCQI_RS12405 (ACFCQI_12405) - 2775316..2777313 (-) 1998 WP_395117122.1 hypothetical protein -
  ACFCQI_RS12410 (ACFCQI_12410) - 2777310..2779073 (-) 1764 WP_395117124.1 tetratricopeptide repeat protein -
  ACFCQI_RS12415 (ACFCQI_12415) pilE 2779494..2779937 (-) 444 WP_395117126.1 pilin Machinery gene
  ACFCQI_RS12420 (ACFCQI_12420) pilE 2780279..2780716 (-) 438 WP_395117128.1 pilin Machinery gene
  ACFCQI_RS12425 (ACFCQI_12425) - 2780858..2782231 (-) 1374 WP_395117130.1 hypothetical protein -
  ACFCQI_RS12430 (ACFCQI_12430) - 2782322..2782723 (-) 402 WP_395117132.1 GtrA family protein -
  ACFCQI_RS12435 (ACFCQI_12435) - 2782713..2783447 (-) 735 WP_395117134.1 class I SAM-dependent methyltransferase -
  ACFCQI_RS12440 (ACFCQI_12440) - 2783437..2784459 (-) 1023 WP_395117136.1 glycosyltransferase family 2 protein -
  ACFCQI_RS12445 (ACFCQI_12445) - 2784584..2784910 (-) 327 WP_395117138.1 type II toxin-antitoxin system HicB family antitoxin -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15513.76 Da        Isoelectric Point: 9.3539

>NTDB_id=1058895 ACFCQI_RS12415 WP_395117126.1 2779494..2779937(-) (pilE) [Rhodanobacter sp. FW102-FHT14D06]
MKSMQKGFTLIELMIVVAIIAILAAIAIPAYQDYLVRSQVSEASTLADGSKTAISEFYSNTGTFPSTNKSAGLAAATDIR
GKYVTSVDAGTNPGIIKVTFGNAANKAINGKFFGLSAITHAGSIEWSCKNATYTTVDTKYLPTSCRQ

Nucleotide


Download         Length: 444 bp        

>NTDB_id=1058895 ACFCQI_RS12415 WP_395117126.1 2779494..2779937(-) (pilE) [Rhodanobacter sp. FW102-FHT14D06]
ATGAAGAGCATGCAGAAAGGCTTTACCCTGATCGAGCTGATGATCGTGGTCGCGATTATCGCGATCCTCGCCGCCATAGC
CATCCCGGCCTACCAAGACTACTTGGTTCGCTCGCAGGTTTCGGAAGCTTCGACCTTGGCCGACGGCTCCAAGACCGCAA
TCTCGGAGTTCTACTCCAACACCGGCACGTTCCCCAGCACCAATAAGTCTGCGGGCCTTGCTGCTGCCACCGACATCCGG
GGCAAGTACGTGACCAGCGTCGATGCTGGCACGAACCCGGGCATCATCAAAGTGACCTTCGGCAATGCTGCGAACAAGGC
GATCAACGGCAAGTTCTTCGGCCTGTCCGCCATCACCCACGCGGGCTCGATCGAGTGGAGCTGCAAGAACGCGACCTACA
CCACGGTCGACACGAAGTACCTGCCGACGTCGTGCCGTCAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.894

100

0.51
  pilE Neisseria gonorrhoeae MS11

45.342

100

0.497
  pilE Neisseria gonorrhoeae strain FA1090

45.625

100

0.497
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.898

100

0.449
  pilA2 Legionella pneumophila strain ERS1305867

45.833

97.959

0.449
  pilA2 Legionella pneumophila str. Paris

45.833

97.959

0.449
  comP Acinetobacter baylyi ADP1

42.667

100

0.435
  pilA Ralstonia pseudosolanacearum GMI1000

38.788

100

0.435
  pilA Acinetobacter baumannii strain A118

42.177

100

0.422
  pilA/pilA1 Eikenella corrodens VA1

39.49

100

0.422
  pilA Pseudomonas aeruginosa PAK

38.312

100

0.401
  pilA Vibrio campbellii strain DS40M4

36.709

100

0.395
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.255

100

0.388
  pilA Vibrio cholerae C6706

35.762

100

0.367
  pilA Vibrio cholerae strain A1552

35.762

100

0.367
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.762

100

0.367

Multiple sequence alignment