Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACFCQI_RS12420 Genome accession   NZ_CP170722
Coordinates   2780279..2780716 (-) Length   145 a.a.
NCBI ID   WP_395117128.1    Uniprot ID   A0AB74US47
Organism   Rhodanobacter sp. FW102-FHT14D06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2775279..2785716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFCQI_RS12405 (ACFCQI_12405) - 2775316..2777313 (-) 1998 WP_395117122.1 hypothetical protein -
  ACFCQI_RS12410 (ACFCQI_12410) - 2777310..2779073 (-) 1764 WP_395117124.1 tetratricopeptide repeat protein -
  ACFCQI_RS12415 (ACFCQI_12415) pilE 2779494..2779937 (-) 444 WP_395117126.1 pilin Machinery gene
  ACFCQI_RS12420 (ACFCQI_12420) pilE 2780279..2780716 (-) 438 WP_395117128.1 pilin Machinery gene
  ACFCQI_RS12425 (ACFCQI_12425) - 2780858..2782231 (-) 1374 WP_395117130.1 hypothetical protein -
  ACFCQI_RS12430 (ACFCQI_12430) - 2782322..2782723 (-) 402 WP_395117132.1 GtrA family protein -
  ACFCQI_RS12435 (ACFCQI_12435) - 2782713..2783447 (-) 735 WP_395117134.1 class I SAM-dependent methyltransferase -
  ACFCQI_RS12440 (ACFCQI_12440) - 2783437..2784459 (-) 1023 WP_395117136.1 glycosyltransferase family 2 protein -
  ACFCQI_RS12445 (ACFCQI_12445) - 2784584..2784910 (-) 327 WP_395117138.1 type II toxin-antitoxin system HicB family antitoxin -
  ACFCQI_RS12450 (ACFCQI_12450) - 2784907..2785155 (-) 249 WP_395117140.1 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15141.37 Da        Isoelectric Point: 8.4601

>NTDB_id=1058896 ACFCQI_RS12420 WP_395117128.1 2780279..2780716(-) (pilE) [Rhodanobacter sp. FW102-FHT14D06]
MQRQHKSAGFTLIELMIVVAIIAILAAIAIPAYQDYLVRAQVTEGLSLASGAKSAVWDYVSNKGEYPPNNQSAGLAPKSD
IHGSYVSSVDVTDGAITVAFQGAKANTAIRSAVLVLSPTTVGGSILWTCAPSTVDGKYLPSSCRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1058896 ACFCQI_RS12420 WP_395117128.1 2780279..2780716(-) (pilE) [Rhodanobacter sp. FW102-FHT14D06]
ATGCAACGCCAGCACAAGTCTGCCGGTTTCACCTTGATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTTGCCGC
CATAGCCATTCCGGCATACCAGGACTATCTGGTCCGTGCCCAAGTCACTGAAGGCCTGAGTCTGGCTTCTGGTGCGAAAT
CGGCCGTGTGGGATTACGTATCCAACAAGGGCGAGTATCCGCCGAACAACCAATCTGCCGGGCTGGCTCCAAAAAGCGAT
ATACACGGCAGCTACGTGTCCAGCGTGGATGTGACTGACGGCGCGATTACTGTGGCGTTCCAAGGTGCCAAAGCCAACAC
GGCTATTCGCAGCGCAGTGCTCGTGCTGTCACCTACCACCGTGGGGGGCAGCATCCTGTGGACTTGCGCCCCCAGTACCG
TCGACGGCAAGTACCTGCCCTCATCCTGCCGAAAGTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

47.059

100

0.497
  pilE Neisseria gonorrhoeae strain FA1090

45.395

100

0.476
  pilA Ralstonia pseudosolanacearum GMI1000

41.615

100

0.462
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.714

100

0.448
  pilA2 Legionella pneumophila str. Paris

44.366

97.931

0.434
  comP Acinetobacter baylyi ADP1

42.466

100

0.428
  pilA2 Legionella pneumophila strain ERS1305867

43.662

97.931

0.428
  pilA/pilA1 Eikenella corrodens VA1

40.789

100

0.428
  pilA Acinetobacter baumannii strain A118

41.727

95.862

0.4
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.007

95.862

0.393
  pilA Pseudomonas aeruginosa PAK

36.601

100

0.386
  pilA Vibrio cholerae C6706

35.099

100

0.366
  pilA Vibrio cholerae strain A1552

35.099

100

0.366
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.099

100

0.366

Multiple sequence alignment