Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFCQI_RS12055 Genome accession   NZ_CP170722
Coordinates   2698923..2699948 (+) Length   341 a.a.
NCBI ID   WP_395116993.1    Uniprot ID   A0AB74UWI5
Organism   Rhodanobacter sp. FW102-FHT14D06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2693923..2704948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFCQI_RS12040 (ACFCQI_12040) - 2694166..2697000 (-) 2835 WP_395116987.1 valine--tRNA ligase -
  ACFCQI_RS12045 (ACFCQI_12045) - 2697178..2697753 (-) 576 WP_395116989.1 YggT family protein -
  ACFCQI_RS12050 (ACFCQI_12050) proC 2697750..2698574 (-) 825 WP_395116991.1 pyrroline-5-carboxylate reductase -
  ACFCQI_RS12055 (ACFCQI_12055) pilT 2698923..2699948 (+) 1026 WP_395116993.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFCQI_RS12060 (ACFCQI_12060) pilU 2700103..2701272 (+) 1170 WP_136256917.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFCQI_RS12065 (ACFCQI_12065) - 2701341..2701751 (-) 411 WP_395116995.1 BON domain-containing protein -
  ACFCQI_RS12070 (ACFCQI_12070) - 2702001..2703785 (-) 1785 WP_395116997.1 hypothetical protein -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38007.59 Da        Isoelectric Point: 6.7268

>NTDB_id=1058889 ACFCQI_RS12055 WP_395116993.1 2698923..2699948(+) (pilT) [Rhodanobacter sp. FW102-FHT14D06]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFFETDFSFEIPGLAR
FRVNAFTQNRGAGAVFRTIPSEVLTLEDLGAPRVFKELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHMLTIED
PIEFVHTSQKCLVNQREVHRDTHGFNEALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRIIDVF
PAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQFGMQTLDQCLLDLV
KRGVVTRQQAQGYAKNKDSFK

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=1058889 ACFCQI_RS12055 WP_395116993.1 2698923..2699948(+) (pilT) [Rhodanobacter sp. FW102-FHT14D06]
ATGGACATTGCCGAACTGCTTGCCTTCTCGGTAAAGAACAAGGCCTCGGATTTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTCCGCCGGATCAACATCCCCGCGCTCGAGCACAAGCAGGTGCACTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAGGAGTTCTTCGAGACCGATTTCTCGTTCGAGATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCACCCAGAACCGCGGCGCCGGCGCAGTGTTCCGCACGATTCCGTCCGAGGTGCTGACGCTGGA
AGACCTGGGCGCGCCGCGCGTGTTCAAGGAGCTGATCGAGCAGCCGCAAGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAATACGGGCACATGCTCACGATCGAGGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACGCATGGTTTCAACGA
GGCGCTGCGCGAGGACCCGGACTACATCCTGGTCGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGGCGCTGACCGCCG
CGGAAACCGGCCATCTGGTGTTCGCCACCGTGCATACAAGTTCGGCGGCCAAGACCATCGACCGCATCATCGACGTGTTC
CCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAAAGCCTGCGCGCGGTGATTTCGCAGTCACTGCTGAAGAA
GGTCGGCGGCGGCCGCATCGCGGCGCACGAGATCATGGTCGGCATCCCGGCCATCCGCAACCTGATCCGCGAGGACAAGG
TGGCGCAGATGTACTCGTCGATCCAGACCGGCCAGCAGTTCGGCATGCAGACGCTGGACCAGTGCCTGCTGGACCTGGTC
AAGCGTGGCGTCGTGACGCGCCAGCAGGCGCAGGGCTACGCCAAGAACAAGGACAGTTTCAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.616

100

0.783
  pilT Acinetobacter baumannii D1279779

77.616

100

0.783
  pilT Acinetobacter baumannii strain A118

77.616

100

0.783
  pilT Pseudomonas stutzeri DSM 10701

76.744

100

0.774
  pilT Pseudomonas aeruginosa PAK

76.453

100

0.771
  pilT Acinetobacter baylyi ADP1

75.291

100

0.76
  pilT Legionella pneumophila strain Lp02

72.965

100

0.736
  pilT Legionella pneumophila strain ERS1305867

72.965

100

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

98.534

0.68
  pilT Vibrio cholerae strain A1552

69.048

98.534

0.68
  pilT Neisseria gonorrhoeae MS11

62.029

100

0.628
  pilT Neisseria meningitidis 8013

62.029

100

0.628
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.338

97.067

0.469
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.405
  pilU Vibrio cholerae strain A1552

39.017

100

0.396
  pilU Acinetobacter baylyi ADP1

38.329

100

0.39

Multiple sequence alignment