Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFYG5_RS11545 Genome accession   NZ_CP170721
Coordinates   2434858..2435895 (-) Length   345 a.a.
NCBI ID   WP_395135988.1    Uniprot ID   A0AB74URB1
Organism   Rhodanobacter sp. FW102-FHT14D07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2429858..2440895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFYG5_RS11530 (ACFYG5_11530) - 2431021..2432805 (+) 1785 WP_395116997.1 hypothetical protein -
  ACFYG5_RS11535 (ACFYG5_11535) - 2433055..2433465 (+) 411 WP_395116995.1 BON domain-containing protein -
  ACFYG5_RS11540 (ACFYG5_11540) pilU 2433534..2434703 (-) 1170 WP_136256917.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFYG5_RS11545 (ACFYG5_11545) pilT 2434858..2435895 (-) 1038 WP_395135988.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFYG5_RS11550 (ACFYG5_11550) proC 2436244..2437068 (+) 825 WP_395116991.1 pyrroline-5-carboxylate reductase -
  ACFYG5_RS11555 (ACFYG5_11555) - 2437065..2437640 (+) 576 WP_395116989.1 YggT family protein -
  ACFYG5_RS11560 (ACFYG5_11560) - 2437818..2440652 (+) 2835 WP_395116987.1 valine--tRNA ligase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38435.09 Da        Isoelectric Point: 6.8886

>NTDB_id=1058867 ACFYG5_RS11545 WP_395135988.1 2434858..2435895(-) (pilT) [Rhodanobacter sp. FW102-FHT14D07]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFFETDFSFEIPGLAR
FRVNAFTQNRGAGAVFRTIPSEVLTLEDLGAPRVFKELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHMLTIED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQFGMQTLDQCL
LDLVKRGVVTRQQAQGYAKNKDSFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1058867 ACFYG5_RS11545 WP_395135988.1 2434858..2435895(-) (pilT) [Rhodanobacter sp. FW102-FHT14D07]
ATGGACATTGCCGAACTGCTTGCCTTCTCGGTAAAGAACAAGGCCTCGGATTTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTCCGCCGGATCAACATCCCCGCGCTCGAGCACAAGCAGGTGCACTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAGGAGTTCTTCGAGACCGATTTCTCGTTCGAGATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCACCCAGAACCGCGGCGCCGGCGCAGTGTTCCGCACGATTCCGTCCGAGGTGCTGACGCTGGA
AGACCTGGGCGCGCCGCGCGTGTTCAAGGAGCTGATCGAGCAGCCGCAAGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAATACGGGCACATGCTCACGATCGAGGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACGCATGGTTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACTACATCCTGGTCGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCATCTGGTGTTCGCCACCGTGCATACAAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAAAGCCTGCGCGCGGTGATTTCGCAGTC
ACTGCTGAAGAAGGTCGGCGGCGGCCGCATCGCGGCGCACGAGATCATGGTCGGCATCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCGATCCAGACCGGCCAGCAGTTCGGCATGCAGACGCTGGACCAGTGCCTG
CTGGACCTGGTCAAGCGTGGCGTCGTGACGCGCCAGCAGGCGCAGGGCTACGCCAAGAACAAGGACAGTTTCAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.616

99.71

0.774
  pilT Acinetobacter baylyi ADP1

76.453

99.71

0.762
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.448

97.101

0.684
  pilT Vibrio cholerae strain A1552

70.448

97.101

0.684
  pilT Neisseria meningitidis 8013

63.188

100

0.632
  pilT Neisseria gonorrhoeae MS11

62.899

100

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.245

95.942

0.472
  pilU Pseudomonas stutzeri DSM 10701

40.173

100

0.403
  pilU Vibrio cholerae strain A1552

39.306

100

0.394
  pilU Acinetobacter baylyi ADP1

38.617

100

0.388

Multiple sequence alignment