Detailed information NTG1058866

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACFYG5_RS11540
• Genome accession: NZ_CP170721
• Coordinates: 2433534..2434703 (-)
• Length: 389 a.a.
• NCBI ID: WP_136256917.1
• Uniprot ID: A0AB74ULL8
• Organism: Rhodanobacter sp. FW102-FHT14D07
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2428534..2439703

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFYG5_RS11515 (ACFYG5_11515)	-	2428770..2429339 (+)	570	WP_395117003.1	YqgE/AlgH family protein	-
ACFYG5_RS11520 (ACFYG5_11520)	ruvX	2429336..2429785 (+)	450	WP_395117001.1	Holliday junction resolvase RuvX	-
ACFYG5_RS11525 (ACFYG5_11525)	-	2429818..2430744 (+)	927	WP_395116999.1	aspartate carbamoyltransferase catalytic subunit	-
ACFYG5_RS11530 (ACFYG5_11530)	-	2431021..2432805 (+)	1785	WP_395116997.1	hypothetical protein	-
ACFYG5_RS11535 (ACFYG5_11535)	-	2433055..2433465 (+)	411	WP_395116995.1	BON domain-containing protein	-
ACFYG5_RS11540 (ACFYG5_11540)	pilU	2433534..2434703 (-)	1170	WP_136256917.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACFYG5_RS11545 (ACFYG5_11545)	pilT	2434858..2435895 (-)	1038	WP_395135988.1	type IV pilus twitching motility protein PilT	Machinery gene
ACFYG5_RS11550 (ACFYG5_11550)	proC	2436244..2437068 (+)	825	WP_395116991.1	pyrroline-5-carboxylate reductase	-
ACFYG5_RS11555 (ACFYG5_11555)	-	2437065..2437640 (+)	576	WP_395116989.1	YggT family protein	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 43258.75 Da
• Isoelectric Point: 6.6350

>NTDB_id=1058866 ACFYG5_RS11540 WP_136256917.1 2433534..2434703(-) (pilU) [Rhodanobacter sp. FW102-FHT14D07]
MSDFDFTSFLKLMVHKKASDLFITAGVAPSMKVQGRIVPITQSPLSVQQARDMVLNVMTPGQREEFEKTHECQFAISAQG
VGRFRVSCFYQRNCVGMVLRRIESKIPTTEELNLPPVIKQLAMTKRGIIIFVGATGSGKSTSLAAMVGYRNLNSTGHIIT
IEDPIEYVHKHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEIRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRILHFFPEDRRQQLLMDLSLNLKGIVAQMLIPTPDGKARRVAVEVLLGTPLAQDYIRQGEIHKLKELMKESTLLGMKTF
DQALVELYHAGEISYEDALRYADSSNEVRLRIKLAQGGDAHTLSQGLDGVELEEDPNDKSFGGSNLLNR

Nucleotide

• Length: 1170 bp

>NTDB_id=1058866 ACFYG5_RS11540 WP_136256917.1 2433534..2434703(-) (pilU) [Rhodanobacter sp. FW102-FHT14D07]
ATGAGCGACTTCGATTTCACCTCGTTCCTGAAACTGATGGTGCACAAGAAGGCATCGGATCTTTTCATCACGGCCGGCGT
GGCGCCGTCGATGAAGGTGCAGGGGCGCATCGTGCCGATCACGCAGAGCCCGCTCAGCGTGCAGCAGGCGCGCGACATGG
TGCTCAACGTGATGACGCCGGGCCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCCAGTTCGCAATTTCCGCGCAGGGC
GTGGGCCGTTTCCGCGTGTCGTGCTTCTACCAGCGCAACTGCGTGGGCATGGTGTTGCGCCGGATCGAGTCGAAGATCCC
CACCACCGAGGAGCTGAACCTGCCGCCGGTGATCAAGCAGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTTCGGGCAAATCGACCTCGCTGGCGGCGATGGTCGGCTACCGCAACCTCAACTCGACAGGGCACATCATCACG
ATCGAGGACCCGATCGAGTACGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGACGCTGCCCTGAAGAACACCCTGCGCCAGGCACCCGACGTGATCATGATCGGCGAAATCCGCACCCGCGAGGGCA
TGGACCACGCCATCGCGTTCGCCGAGACCGGCCACCTGGTGCTGTGCACGCTGCATGCGAACAACGCCAACCAGGCGATG
GACCGCATCCTGCATTTCTTCCCGGAGGATCGCCGCCAGCAGTTGCTGATGGACCTGTCGCTGAACCTCAAGGGCATCGT
GGCGCAGATGCTGATTCCGACGCCGGACGGCAAGGCGCGTCGGGTCGCGGTGGAAGTGCTGCTGGGCACGCCGCTGGCGC
AGGACTACATCCGCCAGGGCGAGATCCACAAGCTGAAGGAGCTGATGAAGGAGTCGACCCTGCTCGGCATGAAGACCTTC
GACCAGGCGCTGGTCGAGCTCTACCACGCCGGCGAGATCAGCTACGAGGATGCGCTGCGCTACGCGGACAGCTCCAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGTGGCGACGCGCACACCTTGTCGCAGGGGCTGGACGGCGTCGAACTGGAAG
AAGACCCCAACGACAAGAGCTTCGGCGGCAGCAACCTGCTCAACCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-247)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	66.404	97.943	0.65
pilU	Acinetobacter baylyi ADP1	63.483	91.517	0.581
pilU	Vibrio cholerae strain A1552	53.09	91.517	0.486
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.247	95.63	0.375