Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACFYG5_RS11185 Genome accession   NZ_CP170721
Coordinates   2354881..2355324 (+) Length   147 a.a.
NCBI ID   WP_395117126.1    Uniprot ID   A0AB74UKE2
Organism   Rhodanobacter sp. FW102-FHT14D07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2355745..2379291 2354881..2355324 flank 421


Gene organization within MGE regions


Location: 2354881..2379291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFYG5_RS11185 (ACFYG5_11185) pilE 2354881..2355324 (+) 444 WP_395117126.1 pilin Machinery gene
  ACFYG5_RS11190 (ACFYG5_11190) - 2355745..2357508 (+) 1764 WP_395117124.1 tetratricopeptide repeat protein -
  ACFYG5_RS11195 (ACFYG5_11195) - 2357505..2359502 (+) 1998 WP_395117122.1 hypothetical protein -
  ACFYG5_RS11200 (ACFYG5_11200) - 2359538..2360659 (+) 1122 WP_395117120.1 glycosyltransferase family 4 protein -
  ACFYG5_RS11205 (ACFYG5_11205) - 2360652..2361578 (+) 927 WP_395117118.1 NAD-dependent epimerase/dehydratase family protein -
  ACFYG5_RS11210 (ACFYG5_11210) - 2361548..2362594 (+) 1047 WP_395117116.1 glycosyltransferase family 2 protein -
  ACFYG5_RS11215 (ACFYG5_11215) - 2362680..2363834 (-) 1155 WP_395117114.1 glycoside hydrolase family 99-like domain-containing protein -
  ACFYG5_RS11220 (ACFYG5_11220) - 2363834..2365087 (-) 1254 WP_395117112.1 class I SAM-dependent methyltransferase -
  ACFYG5_RS11225 (ACFYG5_11225) - 2365084..2366043 (-) 960 WP_395117110.1 GDP-mannose 4,6-dehydratase -
  ACFYG5_RS11230 (ACFYG5_11230) - 2366043..2367068 (-) 1026 WP_395117109.1 glycosyltransferase family 2 protein -
  ACFYG5_RS11235 (ACFYG5_11235) - 2367068..2367919 (-) 852 WP_395117107.1 hypothetical protein -
  ACFYG5_RS11240 (ACFYG5_11240) - 2367938..2369446 (-) 1509 WP_395117105.1 lipopolysaccharide biosynthesis protein -
  ACFYG5_RS11245 (ACFYG5_11245) gmd 2369451..2370545 (-) 1095 WP_395117103.1 GDP-mannose 4,6-dehydratase -
  ACFYG5_RS11250 (ACFYG5_11250) - 2370589..2371446 (-) 858 WP_395117101.1 glycosyltransferase family 2 protein -
  ACFYG5_RS11255 (ACFYG5_11255) - 2371515..2371910 (-) 396 WP_395117099.1 PIN domain-containing protein -
  ACFYG5_RS11260 (ACFYG5_11260) - 2371907..2372146 (-) 240 WP_395117097.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  ACFYG5_RS11265 (ACFYG5_11265) pilB 2372392..2374113 (+) 1722 WP_395117095.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFYG5_RS11270 (ACFYG5_11270) pilC 2374255..2375523 (+) 1269 WP_395135987.1 type II secretion system F family protein Machinery gene
  ACFYG5_RS11275 (ACFYG5_11275) - 2375610..2376002 (+) 393 WP_395117091.1 endonuclease domain-containing protein -
  ACFYG5_RS11280 (ACFYG5_11280) pilD 2376013..2376870 (+) 858 WP_395117089.1 prepilin peptidase Machinery gene
  ACFYG5_RS11285 (ACFYG5_11285) coaE 2376867..2377478 (+) 612 WP_395117087.1 dephospho-CoA kinase -
  ACFYG5_RS11290 (ACFYG5_11290) - 2377498..2377896 (-) 399 WP_395117085.1 NUDIX domain-containing protein -
  ACFYG5_RS11295 (ACFYG5_11295) - 2377896..2378384 (-) 489 WP_395117083.1 dihydrofolate reductase -
  ACFYG5_RS11300 (ACFYG5_11300) - 2378476..2379291 (-) 816 WP_395117081.1 thymidylate synthase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15513.76 Da        Isoelectric Point: 9.3539

>NTDB_id=1058861 ACFYG5_RS11185 WP_395117126.1 2354881..2355324(+) (pilE) [Rhodanobacter sp. FW102-FHT14D07]
MKSMQKGFTLIELMIVVAIIAILAAIAIPAYQDYLVRSQVSEASTLADGSKTAISEFYSNTGTFPSTNKSAGLAAATDIR
GKYVTSVDAGTNPGIIKVTFGNAANKAINGKFFGLSAITHAGSIEWSCKNATYTTVDTKYLPTSCRQ

Nucleotide


Download         Length: 444 bp        

>NTDB_id=1058861 ACFYG5_RS11185 WP_395117126.1 2354881..2355324(+) (pilE) [Rhodanobacter sp. FW102-FHT14D07]
ATGAAGAGCATGCAGAAAGGCTTTACCCTGATCGAGCTGATGATCGTGGTCGCGATTATCGCGATCCTCGCCGCCATAGC
CATCCCGGCCTACCAAGACTACTTGGTTCGCTCGCAGGTTTCGGAAGCTTCGACCTTGGCCGACGGCTCCAAGACCGCAA
TCTCGGAGTTCTACTCCAACACCGGCACGTTCCCCAGCACCAATAAGTCTGCGGGCCTTGCTGCTGCCACCGACATCCGG
GGCAAGTACGTGACCAGCGTCGATGCTGGCACGAACCCGGGCATCATCAAAGTGACCTTCGGCAATGCTGCGAACAAGGC
GATCAACGGCAAGTTCTTCGGCCTGTCCGCCATCACCCACGCGGGCTCGATCGAGTGGAGCTGCAAGAACGCGACCTACA
CCACGGTCGACACGAAGTACCTGCCGACGTCGTGCCGTCAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.894

100

0.51
  pilE Neisseria gonorrhoeae MS11

45.342

100

0.497
  pilE Neisseria gonorrhoeae strain FA1090

45.625

100

0.497
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.898

100

0.449
  pilA2 Legionella pneumophila strain ERS1305867

45.833

97.959

0.449
  pilA2 Legionella pneumophila str. Paris

45.833

97.959

0.449
  comP Acinetobacter baylyi ADP1

42.667

100

0.435
  pilA Ralstonia pseudosolanacearum GMI1000

38.788

100

0.435
  pilA Acinetobacter baumannii strain A118

42.177

100

0.422
  pilA/pilA1 Eikenella corrodens VA1

39.49

100

0.422
  pilA Pseudomonas aeruginosa PAK

38.312

100

0.401
  pilA Vibrio campbellii strain DS40M4

36.709

100

0.395
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.255

100

0.388
  pilA Vibrio cholerae C6706

35.762

100

0.367
  pilA Vibrio cholerae strain A1552

35.762

100

0.367
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

35.762

100

0.367

Multiple sequence alignment