Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFYG5_RS11265 Genome accession   NZ_CP170721
Coordinates   2372392..2374113 (+) Length   573 a.a.
NCBI ID   WP_395117095.1    Uniprot ID   A0AB74UPI1
Organism   Rhodanobacter sp. FW102-FHT14D07     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2355745..2379291 2372392..2374113 within 0


Gene organization within MGE regions


Location: 2355745..2379291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFYG5_RS11190 (ACFYG5_11190) - 2355745..2357508 (+) 1764 WP_395117124.1 tetratricopeptide repeat protein -
  ACFYG5_RS11195 (ACFYG5_11195) - 2357505..2359502 (+) 1998 WP_395117122.1 hypothetical protein -
  ACFYG5_RS11200 (ACFYG5_11200) - 2359538..2360659 (+) 1122 WP_395117120.1 glycosyltransferase family 4 protein -
  ACFYG5_RS11205 (ACFYG5_11205) - 2360652..2361578 (+) 927 WP_395117118.1 NAD-dependent epimerase/dehydratase family protein -
  ACFYG5_RS11210 (ACFYG5_11210) - 2361548..2362594 (+) 1047 WP_395117116.1 glycosyltransferase family 2 protein -
  ACFYG5_RS11215 (ACFYG5_11215) - 2362680..2363834 (-) 1155 WP_395117114.1 glycoside hydrolase family 99-like domain-containing protein -
  ACFYG5_RS11220 (ACFYG5_11220) - 2363834..2365087 (-) 1254 WP_395117112.1 class I SAM-dependent methyltransferase -
  ACFYG5_RS11225 (ACFYG5_11225) - 2365084..2366043 (-) 960 WP_395117110.1 GDP-mannose 4,6-dehydratase -
  ACFYG5_RS11230 (ACFYG5_11230) - 2366043..2367068 (-) 1026 WP_395117109.1 glycosyltransferase family 2 protein -
  ACFYG5_RS11235 (ACFYG5_11235) - 2367068..2367919 (-) 852 WP_395117107.1 hypothetical protein -
  ACFYG5_RS11240 (ACFYG5_11240) - 2367938..2369446 (-) 1509 WP_395117105.1 lipopolysaccharide biosynthesis protein -
  ACFYG5_RS11245 (ACFYG5_11245) gmd 2369451..2370545 (-) 1095 WP_395117103.1 GDP-mannose 4,6-dehydratase -
  ACFYG5_RS11250 (ACFYG5_11250) - 2370589..2371446 (-) 858 WP_395117101.1 glycosyltransferase family 2 protein -
  ACFYG5_RS11255 (ACFYG5_11255) - 2371515..2371910 (-) 396 WP_395117099.1 PIN domain-containing protein -
  ACFYG5_RS11260 (ACFYG5_11260) - 2371907..2372146 (-) 240 WP_395117097.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  ACFYG5_RS11265 (ACFYG5_11265) pilB 2372392..2374113 (+) 1722 WP_395117095.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFYG5_RS11270 (ACFYG5_11270) pilC 2374255..2375523 (+) 1269 WP_395135987.1 type II secretion system F family protein Machinery gene
  ACFYG5_RS11275 (ACFYG5_11275) - 2375610..2376002 (+) 393 WP_395117091.1 endonuclease domain-containing protein -
  ACFYG5_RS11280 (ACFYG5_11280) pilD 2376013..2376870 (+) 858 WP_395117089.1 prepilin peptidase Machinery gene
  ACFYG5_RS11285 (ACFYG5_11285) coaE 2376867..2377478 (+) 612 WP_395117087.1 dephospho-CoA kinase -
  ACFYG5_RS11290 (ACFYG5_11290) - 2377498..2377896 (-) 399 WP_395117085.1 NUDIX domain-containing protein -
  ACFYG5_RS11295 (ACFYG5_11295) - 2377896..2378384 (-) 489 WP_395117083.1 dihydrofolate reductase -
  ACFYG5_RS11300 (ACFYG5_11300) - 2378476..2379291 (-) 816 WP_395117081.1 thymidylate synthase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63052.88 Da        Isoelectric Point: 6.2666

>NTDB_id=1058862 ACFYG5_RS11265 WP_395117095.1 2372392..2374113(+) (pilB) [Rhodanobacter sp. FW102-FHT14D07]
MSSQPQPSLAGLTGMARRLVSEGLLPEDDVRKAMVDSTQRKVSLGAWLLDHNLVENAQLTRVLSLEFGMPMMDVTSLNPT
TMPLDLISEALMTKHQALPLFRRGKRLFVGIADPTQSHALDEIKFHSNCLVEPILVERSSLQRTIENLLNSMRDNMPDMD
TGEFDELDLEVGSDEAESTGIDANATDDAPVVKFVNKILVDAIRRGASDIHFEPFETQYRVRLRMDGMLKAVASPPMKMA
NRISSRLKVMAQLDIAEKRVPQDGRIKLNLSKTRAIDFRVSSLPTLFGEKIVLRILDGSAARIGIEKLGYEPEQQKLYVD
AIHKPYGMVLVTGPTGSGKTVSLYTGLNMLNTAERNISTVEDPVEIRVEGINQVQQNAKRGMTFASALRSFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAAQTIARLMNMGIAPYNITSSVTLIIAQRLARRLHECKKPIELPPKVLL
DAGFSQADIDAGLTIYQAVGCEGCNEGYKGRVGIYQVMPMLEDIQKIILQGGNAMQIAEVAKAAGVNDLRASALLKVKQG
LTSLTEIDRVTKE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1058862 ACFYG5_RS11265 WP_395117095.1 2372392..2374113(+) (pilB) [Rhodanobacter sp. FW102-FHT14D07]
ATGTCATCGCAACCGCAACCGTCGCTCGCAGGCCTGACCGGCATGGCGCGCCGCCTGGTGTCCGAAGGGCTGCTGCCCGA
GGATGACGTTCGCAAGGCGATGGTCGACAGCACGCAACGCAAGGTGTCGCTGGGCGCGTGGCTGCTGGATCACAACCTGG
TCGAGAACGCGCAGCTGACGCGGGTGCTGTCGCTCGAGTTCGGCATGCCGATGATGGACGTGACCTCCCTCAACCCGACC
ACGATGCCGCTGGACCTGATCAGCGAGGCGCTGATGACCAAGCATCAGGCGCTGCCGCTGTTCCGGCGCGGCAAGCGCCT
GTTCGTGGGCATCGCCGACCCGACCCAGTCGCACGCGCTGGACGAGATCAAGTTCCATTCCAATTGCCTGGTCGAGCCGA
TCCTGGTCGAGCGCAGTTCGCTGCAGCGGACGATCGAGAACCTGCTGAACTCCATGCGCGACAACATGCCGGACATGGAT
ACCGGCGAGTTCGACGAGCTGGATCTGGAGGTGGGCAGCGACGAGGCCGAATCCACCGGCATCGACGCCAATGCCACGGA
CGACGCCCCGGTGGTGAAGTTCGTCAACAAGATCCTGGTCGACGCGATTCGCCGCGGCGCCTCGGACATCCATTTCGAAC
CGTTCGAAACCCAGTACCGGGTGCGCCTGCGCATGGACGGCATGTTGAAGGCCGTGGCCAGCCCGCCGATGAAGATGGCC
AACCGGATCTCCTCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCGCATCAA
GCTCAACCTCTCCAAGACCCGCGCGATCGACTTTCGCGTCAGCTCGCTGCCGACGCTGTTCGGCGAGAAGATCGTGCTGC
GTATCCTCGACGGTTCGGCGGCCAGGATCGGCATCGAGAAACTGGGCTACGAGCCGGAGCAGCAGAAGCTCTACGTCGAT
GCGATCCACAAGCCTTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACACCGGCCT
GAACATGCTCAACACCGCCGAGCGCAACATCTCCACGGTGGAGGACCCGGTCGAAATCCGCGTCGAGGGCATCAACCAGG
TGCAGCAGAACGCCAAGCGCGGCATGACCTTCGCCAGCGCATTGCGTTCGTTCCTTCGCCAGGACCCGGACGTGATCATG
GTCGGCGAAATCCGTGACCTGGAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCCACCCT
GCACACCAACGACGCGGCGCAGACGATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCTCGTCGGTGA
CCCTGATCATCGCCCAGCGCCTGGCCCGGCGCCTGCACGAATGCAAGAAGCCGATCGAGCTGCCGCCGAAGGTGCTGCTG
GATGCCGGCTTCAGCCAGGCCGACATCGACGCCGGCCTGACCATCTACCAGGCCGTGGGTTGTGAGGGCTGCAACGAGGG
CTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGATGCTGGAGGATATCCAGAAGATCATCCTGCAGGGCGGCAATG
CCATGCAGATCGCCGAAGTGGCCAAGGCCGCCGGGGTCAACGACCTGCGCGCCTCGGCCCTGCTCAAGGTGAAGCAGGGG
CTGACCAGTCTCACCGAGATCGACCGGGTGACCAAGGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-153)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.044

98.604

0.543
  pilB Acinetobacter baumannii D1279779

53.901

98.429

0.531
  pilB Legionella pneumophila strain ERS1305867

50.442

98.604

0.497
  pilB Vibrio campbellii strain DS40M4

47.173

98.778

0.466
  pilB Vibrio cholerae strain A1552

50.189

92.496

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

48.881

93.543

0.457
  pilF Neisseria gonorrhoeae MS11

48.282

91.449

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.605

100

0.389
  pilF Thermus thermophilus HB27

38.146

97.906

0.373

Multiple sequence alignment