Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFJIT_RS02590 Genome accession   NZ_CP170559
Coordinates   603125..604162 (+) Length   345 a.a.
NCBI ID   WP_130517185.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. UC19_8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 598125..609162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIT_RS02565 (ACFJIT_02565) - 598551..599918 (-) 1368 WP_379654933.1 MFS transporter -
  ACFJIT_RS02570 (ACFJIT_02570) soxR 600013..600447 (+) 435 WP_379654934.1 redox-sensitive transcriptional activator SoxR -
  ACFJIT_RS02575 (ACFJIT_02575) - 600467..600937 (-) 471 WP_379654935.1 DUF4426 domain-containing protein -
  ACFJIT_RS02580 (ACFJIT_02580) proC 600943..601794 (-) 852 WP_379654936.1 pyrroline-5-carboxylate reductase -
  ACFJIT_RS02585 (ACFJIT_02585) - 602343..603023 (-) 681 WP_379654937.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJIT_RS02590 (ACFJIT_02590) pilT 603125..604162 (+) 1038 WP_130517185.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJIT_RS02595 (ACFJIT_02595) pilU 604290..605420 (+) 1131 WP_130517187.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJIT_RS02600 (ACFJIT_02600) - 605443..605943 (+) 501 WP_379654938.1 RHS repeat domain-containing protein -
  ACFJIT_RS02605 (ACFJIT_02605) - 606319..606630 (+) 312 WP_379654939.1 DUF5076 domain-containing protein -
  ACFJIT_RS02610 (ACFJIT_02610) - 606928..607623 (+) 696 WP_379655698.1 YitT family protein -
  ACFJIT_RS02615 (ACFJIT_02615) - 608181..608750 (-) 570 WP_379654940.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38709.43 Da        Isoelectric Point: 6.7269

>NTDB_id=1058013 ACFJIT_RS02590 WP_130517185.1 603125..604162(+) (pilT) [Pseudoxanthomonas sp. UC19_8]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREMVQQPQGLILVTGPTGSGKSTTLAAMIDYINKSEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSQITRHQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1058013 ACFJIT_RS02590 WP_130517185.1 603125..604162(+) (pilT) [Pseudoxanthomonas sp. UC19_8]
ATGGATATCGCCGAACTACTGGCCTTCTCGGTCAAGAACAAGGCCTCGGACCTGCATCTGTCCGCCGGGCTGCCGCCGAT
GATCCGCGTGGATGGCGACGTGCGCCGCATCAACATCCCCGCCCTGGACCACAAGCAGGTCCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTTGAGGTCGACTTCTCCTTCGAGATCCCCTCGCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATCCCGTCCGAGGTGCTGACCCTGGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAGATGGTGCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAGCGAATACGGCCATATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCGCTGCGCGAGGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTAATCAGCCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCAGATCACGCGCCACCAGGCCAAGGAATACGCCAAGGACAAGCGGTTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.103

98.841

0.643
  pilT Neisseria gonorrhoeae MS11

64.809

98.841

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.118

98.261

0.394
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment