Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFJIT_RS02595 Genome accession   NZ_CP170559
Coordinates   604290..605420 (+) Length   376 a.a.
NCBI ID   WP_130517187.1    Uniprot ID   A0A4Q9TBT5
Organism   Pseudoxanthomonas sp. UC19_8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 599290..610420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIT_RS02570 (ACFJIT_02570) soxR 600013..600447 (+) 435 WP_379654934.1 redox-sensitive transcriptional activator SoxR -
  ACFJIT_RS02575 (ACFJIT_02575) - 600467..600937 (-) 471 WP_379654935.1 DUF4426 domain-containing protein -
  ACFJIT_RS02580 (ACFJIT_02580) proC 600943..601794 (-) 852 WP_379654936.1 pyrroline-5-carboxylate reductase -
  ACFJIT_RS02585 (ACFJIT_02585) - 602343..603023 (-) 681 WP_379654937.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJIT_RS02590 (ACFJIT_02590) pilT 603125..604162 (+) 1038 WP_130517185.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJIT_RS02595 (ACFJIT_02595) pilU 604290..605420 (+) 1131 WP_130517187.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJIT_RS02600 (ACFJIT_02600) - 605443..605943 (+) 501 WP_379654938.1 RHS repeat domain-containing protein -
  ACFJIT_RS02605 (ACFJIT_02605) - 606319..606630 (+) 312 WP_379654939.1 DUF5076 domain-containing protein -
  ACFJIT_RS02610 (ACFJIT_02610) - 606928..607623 (+) 696 WP_379655698.1 YitT family protein -
  ACFJIT_RS02615 (ACFJIT_02615) - 608181..608750 (-) 570 WP_379654940.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41848.14 Da        Isoelectric Point: 6.8286

>NTDB_id=1058014 ACFJIT_RS02595 WP_130517187.1 604290..605420(+) (pilU) [Pseudoxanthomonas sp. UC19_8]
MSAIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKLTPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLVPTPDGKARRVAMEIMLGTPLIQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAQGLDGVEVAEAR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1058014 ACFJIT_RS02595 WP_130517187.1 604290..605420(+) (pilU) [Pseudoxanthomonas sp. UC19_8]
ATGAGCGCCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGATCTGTTCATCACCTCGGGCAT
GCCGCCGTCGATCAAGGTGCATGGCAAGCTCACTCCGATCACGCAGACGCCGCTGACGCCGCAGCAGTCGCGCGACCTGG
TGCTCAACGTCATGACCCCCGCCCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGTATGGTGCTGCGCCGGATCGAGACCAAGATCCC
CAGCGTCGATGAGCTCAACCTGCCGCCGGTCATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGTCACATCATTACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTTGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGTGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTCTCGCTCAACCTCAAGGGCGTGGT
CGCCCAGCAGCTGGTGCCGACCCCCGACGGCAAGGCGCGCCGGGTGGCGATGGAGATCATGCTGGGCACGCCGCTGATCC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCTCCTACGAGGACGCCCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCCCGCACCCTGGCCCAGGGCCTGGACGGCGTCGAGGTCGCCG
AGGCGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Q9TBT5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.429

93.085

0.386
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Legionella pneumophila strain Lp02

40.896

89.096

0.364
  pilT Legionella pneumophila strain ERS1305867

40.896

89.096

0.364
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362

Multiple sequence alignment