Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFJIS_RS12125 Genome accession   NZ_CP170558
Coordinates   2563030..2564073 (-) Length   347 a.a.
NCBI ID   WP_062482334.1    Uniprot ID   A0A554RS02
Organism   Variovorax boronicumulans strain UC19_11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2558030..2569073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIS_RS12100 (ACFJIS_12100) - 2558966..2559338 (+) 373 Protein_2400 YraN family protein -
  ACFJIS_RS12105 (ACFJIS_12105) - 2559424..2560065 (+) 642 WP_062482322.1 SIS domain-containing protein -
  ACFJIS_RS12110 (ACFJIS_12110) - 2560062..2560856 (+) 795 WP_062482325.1 BON domain-containing protein -
  ACFJIS_RS12115 (ACFJIS_12115) - 2560887..2561795 (-) 909 WP_390348279.1 NAD(P)-dependent oxidoreductase -
  ACFJIS_RS12120 (ACFJIS_12120) pilU 2561864..2563000 (-) 1137 WP_062482331.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJIS_RS12125 (ACFJIS_12125) pilT 2563030..2564073 (-) 1044 WP_062482334.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJIS_RS12130 (ACFJIS_12130) - 2564125..2564826 (+) 702 WP_390348282.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJIS_RS12135 (ACFJIS_12135) - 2564832..2565569 (-) 738 WP_307588150.1 hypothetical protein -
  ACFJIS_RS12140 (ACFJIS_12140) - 2565667..2566135 (-) 469 Protein_2408 Lrp/AsnC ligand binding domain-containing protein -
  ACFJIS_RS12145 (ACFJIS_12145) - 2566279..2566734 (-) 456 WP_095950083.1 hypothetical protein -
  ACFJIS_RS12150 (ACFJIS_12150) - 2566987..2568261 (+) 1275 WP_070063824.1 Glu/Leu/Phe/Val dehydrogenase -
  ACFJIS_RS12155 (ACFJIS_12155) - 2568347..2569051 (+) 705 WP_390348285.1 DUF2242 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38132.83 Da        Isoelectric Point: 6.8356

>NTDB_id=1058006 ACFJIS_RS12125 WP_062482334.1 2563030..2564073(-) (pilT) [Variovorax boronicumulans strain UC19_11]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFANALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1058006 ACFJIS_RS12125 WP_062482334.1 2563030..2564073(-) (pilT) [Variovorax boronicumulans strain UC19_11]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGAACGGCGACGTGCGCCGCATCAACGTCGATGCGCTGGACCACAAGGCGGTGCATGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAATTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTTCGCGTGAACGCCTTCAACCAGGCACGCGGCGCGGCCGCGGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTCAACGCGCCGAAGATTTTCGGCGAACTCGCGCTCAAGCCGCGCGGCCTCGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCGACCACACTGGCGGCGATGGTGAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGATGACGCTGTCGTTCGCCAA
CGCATTGCGCTCCGCCCTGCGCGAAGACCCGGACGCGATCCTGGTGGGCGAGTTGCGCGACCTCGAAACCATTCGCCTCG
CGATGACCGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAGAAAGAAATGATCCGCGCGATGCTGTCGGAGTCTTTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTCGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTCGACCAGAAC
CTGACGGAGCTGGTGCGCCGCAATACGATCTCTGCCGCCGAAGCGCGCGGCAAGGCCAAGATCCCAGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554RS02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Vibrio cholerae strain A1552

68.915

98.271

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.915

98.271

0.677
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment