Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFJIS_RS12120 Genome accession   NZ_CP170558
Coordinates   2561864..2563000 (-) Length   378 a.a.
NCBI ID   WP_062482331.1    Uniprot ID   A0A554RS03
Organism   Variovorax boronicumulans strain UC19_11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2556864..2568000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIS_RS12085 (ACFJIS_12085) - 2556884..2557111 (+) 228 WP_062481748.1 hypothetical protein -
  ACFJIS_RS12090 (ACFJIS_12090) - 2557201..2557929 (+) 729 WP_159274683.1 glutathione S-transferase -
  ACFJIS_RS12095 (ACFJIS_12095) rsmI 2557933..2558919 (-) 987 WP_390348277.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACFJIS_RS12100 (ACFJIS_12100) - 2558966..2559338 (+) 373 Protein_2400 YraN family protein -
  ACFJIS_RS12105 (ACFJIS_12105) - 2559424..2560065 (+) 642 WP_062482322.1 SIS domain-containing protein -
  ACFJIS_RS12110 (ACFJIS_12110) - 2560062..2560856 (+) 795 WP_062482325.1 BON domain-containing protein -
  ACFJIS_RS12115 (ACFJIS_12115) - 2560887..2561795 (-) 909 WP_390348279.1 NAD(P)-dependent oxidoreductase -
  ACFJIS_RS12120 (ACFJIS_12120) pilU 2561864..2563000 (-) 1137 WP_062482331.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJIS_RS12125 (ACFJIS_12125) pilT 2563030..2564073 (-) 1044 WP_062482334.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJIS_RS12130 (ACFJIS_12130) - 2564125..2564826 (+) 702 WP_390348282.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJIS_RS12135 (ACFJIS_12135) - 2564832..2565569 (-) 738 WP_307588150.1 hypothetical protein -
  ACFJIS_RS12140 (ACFJIS_12140) - 2565667..2566135 (-) 469 Protein_2408 Lrp/AsnC ligand binding domain-containing protein -
  ACFJIS_RS12145 (ACFJIS_12145) - 2566279..2566734 (-) 456 WP_095950083.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41796.88 Da        Isoelectric Point: 6.3136

>NTDB_id=1058005 ACFJIS_RS12120 WP_062482331.1 2561864..2563000(-) (pilU) [Variovorax boronicumulans strain UC19_11]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVAMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFEGNAINFEEAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1058005 ACFJIS_RS12120 WP_062482331.1 2561864..2563000(-) (pilU) [Variovorax boronicumulans strain UC19_11]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCACTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATT
TCGCCGACGGGCATCGGGCGCTTTCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTCAAGGACGTGGCGATGACCAAGCGCGGCCTCACCA
TCCTCGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAAAATTCGTACGGC
CACATCGTCACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTCATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCCGTGGCCTTCGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGTTCGCTGGTCTCGCAGCGGCTGGTGCCCACCGAAGACGGCCAGGGCCGCGTGGCCGCCGTCGAGATCCTGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGGGCAACGCGATCAACTTCGAAGAGGCGATCCGCAACGCCGA
TTCGGCCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCGGCCGGCACCGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554RS03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Legionella pneumophila strain ERS1305867

44.681

87.037

0.389
  pilT Legionella pneumophila strain Lp02

44.681

87.037

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment