Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFJIW_RS08945 Genome accession   NZ_CP170554
Coordinates   2176165..2177202 (+) Length   345 a.a.
NCBI ID   WP_386070752.1    Uniprot ID   -
Organism   Tahibacter sp. UC22_41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2171165..2182202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIW_RS08915 (ACFJIW_08915) - 2171798..2172286 (+) 489 WP_386070746.1 TPM domain-containing protein -
  ACFJIW_RS08920 (ACFJIW_08920) - 2172527..2173240 (-) 714 WP_386070747.1 histidine phosphatase family protein -
  ACFJIW_RS08925 (ACFJIW_08925) - 2173237..2173689 (-) 453 WP_386070748.1 DUF4426 domain-containing protein -
  ACFJIW_RS08930 (ACFJIW_08930) - 2173740..2174318 (-) 579 WP_386070749.1 YggT family protein -
  ACFJIW_RS08935 (ACFJIW_08935) proC 2174321..2175163 (-) 843 WP_386070750.1 pyrroline-5-carboxylate reductase -
  ACFJIW_RS08940 (ACFJIW_08940) - 2175394..2176074 (-) 681 WP_386070751.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJIW_RS08945 (ACFJIW_08945) pilT 2176165..2177202 (+) 1038 WP_386070752.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJIW_RS08950 (ACFJIW_08950) pilU 2177342..2178469 (+) 1128 WP_386070753.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJIW_RS08955 (ACFJIW_08955) - 2178477..2178734 (+) 258 WP_386070754.1 hypothetical protein -
  ACFJIW_RS08960 (ACFJIW_08960) - 2178810..2180537 (-) 1728 WP_386070755.1 hypothetical protein -
  ACFJIW_RS08965 (ACFJIW_08965) - 2180634..2181584 (-) 951 WP_386070756.1 aspartate carbamoyltransferase catalytic subunit -
  ACFJIW_RS08970 (ACFJIW_08970) ruvX 2181581..2182000 (-) 420 WP_386070757.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.09 Da        Isoelectric Point: 7.0665

>NTDB_id=1057959 ACFJIW_RS08945 WP_386070752.1 2176165..2177202(+) (pilT) [Tahibacter sp. UC22_41]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQIHSLIYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPKVFKELISQPQGLILVTGPTGSGKSTTLAAMVDHVNKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGAQYGMQTLDQCL
LDLVKRGLVTRPQAASYAKNKEIFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1057959 ACFJIW_RS08945 WP_386070752.1 2176165..2177202(+) (pilT) [Tahibacter sp. UC22_41]
ATGGATATCGCTGAGCTGCTGGCATTCTCAGTCAAGAATAAGGCCTCGGACCTGCACTTGTCCGCGGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGCATCAACATTCCGGCGCTGGACCACAAGCAGATCCACTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAAGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGTACGATTCCCTCGGAAGTGCTGACCCTCGA
AGACCTCGGCTGTCCCAAGGTGTTCAAGGAACTGATCTCGCAGCCGCAGGGGCTGATCCTCGTGACCGGCCCGACGGGCT
CGGGCAAGTCGACGACGCTCGCGGCGATGGTCGACCACGTCAACAAGAACGAATACGCGCACATCCTCTCGGTCGAGGAT
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCATCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCACTGCGCGAAGACCCGGACTACATCCTCGTCGGCGAGTTGCGCGACCTCGAAACGATCCGCCTCG
CGCTGACCGCCGCGGAAACCGGCCATCTGGTGTTCGCGACCCTGCATACCTCCTCGGCGGCCAAGACCGTCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAACCGATGGTGCGTTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGTC
GCTGCTGAAGAAGGTCGGCGGCGGGCGCATCGCGGCGCACGAGATCATGGTCGGCATACCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGTCGATCCAGACCGGCGCCCAGTACGGCATGCAGACCCTGGACCAGTGCCTG
CTCGATCTGGTCAAGCGCGGTCTGGTCACGCGGCCGCAGGCGGCCAGCTACGCCAAGAACAAGGAAATCTTCAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

72.965

99.71

0.728
  pilT Legionella pneumophila strain ERS1305867

72.965

99.71

0.728
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

95.362

0.681
  pilT Vibrio cholerae strain A1552

71.429

95.362

0.681
  pilT Neisseria meningitidis 8013

64.14

99.42

0.638
  pilT Neisseria gonorrhoeae MS11

63.848

99.42

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.547

95.942

0.475
  pilU Pseudomonas stutzeri DSM 10701

41.003

98.261

0.403
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38

Multiple sequence alignment