Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFJIW_RS08950 Genome accession   NZ_CP170554
Coordinates   2177342..2178469 (+) Length   375 a.a.
NCBI ID   WP_386070753.1    Uniprot ID   -
Organism   Tahibacter sp. UC22_41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2172342..2183469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIW_RS08920 (ACFJIW_08920) - 2172527..2173240 (-) 714 WP_386070747.1 histidine phosphatase family protein -
  ACFJIW_RS08925 (ACFJIW_08925) - 2173237..2173689 (-) 453 WP_386070748.1 DUF4426 domain-containing protein -
  ACFJIW_RS08930 (ACFJIW_08930) - 2173740..2174318 (-) 579 WP_386070749.1 YggT family protein -
  ACFJIW_RS08935 (ACFJIW_08935) proC 2174321..2175163 (-) 843 WP_386070750.1 pyrroline-5-carboxylate reductase -
  ACFJIW_RS08940 (ACFJIW_08940) - 2175394..2176074 (-) 681 WP_386070751.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJIW_RS08945 (ACFJIW_08945) pilT 2176165..2177202 (+) 1038 WP_386070752.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJIW_RS08950 (ACFJIW_08950) pilU 2177342..2178469 (+) 1128 WP_386070753.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJIW_RS08955 (ACFJIW_08955) - 2178477..2178734 (+) 258 WP_386070754.1 hypothetical protein -
  ACFJIW_RS08960 (ACFJIW_08960) - 2178810..2180537 (-) 1728 WP_386070755.1 hypothetical protein -
  ACFJIW_RS08965 (ACFJIW_08965) - 2180634..2181584 (-) 951 WP_386070756.1 aspartate carbamoyltransferase catalytic subunit -
  ACFJIW_RS08970 (ACFJIW_08970) ruvX 2181581..2182000 (-) 420 WP_386070757.1 Holliday junction resolvase RuvX -
  ACFJIW_RS08975 (ACFJIW_08975) - 2182086..2182652 (-) 567 WP_386070758.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41850.09 Da        Isoelectric Point: 6.9601

>NTDB_id=1057960 ACFJIW_RS08950 WP_386070753.1 2177342..2178469(+) (pilU) [Tahibacter sp. UC22_41]
MSSVDFTSFLKLMVHKKASDLFITAGVAPSMKVHGRISPITQNPLTPQQSRDMVLNVMTPSQREEFEKTHECQFAISVTG
VGRFRVSCFYQRNCVGMVLRRIETKIPTVDELALPAVIKTLAMTKRGIIIFVGATGTGKSTSLAAMVGYRNQNSTGHIIT
IEDPIEYVHKHEGCIITQRELGIDTDSWENALKNTLRQAPDVILIGEVRTRETMEHAINFSETGHLCLCTLHANNANQAL
DRIIHFFGEDRREQLFMDLSLNLKGVVAQQLIPTPDGKARRVAMEILLGTPLVQDYVRKGEVHKLKDVMKESTNLGMRTF
DQSLFELYQAGEISYEDALRHADSANEVRLRIKLAQGGDAHTLSQGLDGVELMES

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1057960 ACFJIW_RS08950 WP_386070753.1 2177342..2178469(+) (pilU) [Tahibacter sp. UC22_41]
ATGAGCAGCGTAGATTTCACCTCGTTTCTGAAGCTGATGGTGCACAAGAAGGCATCGGATCTTTTCATCACGGCCGGCGT
CGCGCCGTCGATGAAGGTCCACGGCCGCATCAGTCCGATCACGCAGAATCCGCTGACGCCGCAGCAGTCGCGCGACATGG
TGCTCAACGTCATGACGCCGTCGCAGCGCGAGGAGTTCGAAAAGACGCACGAGTGCCAGTTCGCGATCAGCGTGACCGGC
GTCGGCCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACTGCGTCGGCATGGTGCTGCGCCGTATCGAGACCAAGATCCC
GACCGTCGACGAGCTCGCGCTGCCGGCCGTGATCAAGACCTTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CGACCGGTACCGGTAAGTCGACCTCGCTCGCGGCCATGGTCGGCTATCGCAACCAGAATTCGACCGGCCACATCATCACG
ATCGAGGACCCGATCGAATACGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAACTCGGCATCGACACCGACTC
CTGGGAGAACGCGCTGAAGAACACTCTGCGTCAGGCGCCCGACGTGATCCTGATCGGCGAAGTGCGCACGCGCGAGACGA
TGGAGCACGCGATCAACTTCTCCGAAACCGGCCATCTGTGCCTGTGCACCCTGCACGCGAACAACGCGAACCAGGCGCTG
GACCGCATCATCCACTTCTTCGGCGAGGACCGCCGCGAGCAGCTGTTCATGGACCTGTCCCTGAACCTCAAGGGCGTCGT
CGCGCAGCAGCTGATTCCGACGCCGGACGGCAAGGCGCGCCGCGTCGCGATGGAAATCCTGCTCGGCACGCCGCTGGTGC
AGGACTACGTCCGCAAGGGCGAGGTGCACAAGCTCAAGGACGTCATGAAGGAGTCGACCAATCTCGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTGTATCAGGCTGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGGCCAACGA
AGTCCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCACATACCCTGAGCCAGGGACTCGACGGCGTCGAGCTCATGG
AAAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.867

0.661
  pilU Acinetobacter baylyi ADP1

62.465

95.2

0.595
  pilU Vibrio cholerae strain A1552

53.09

94.933

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.516

99.2

0.392

Multiple sequence alignment