Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFJI0_RS20840 Genome accession   NZ_CP170552
Coordinates   4149558..4150601 (+) Length   347 a.a.
NCBI ID   WP_291121788.1    Uniprot ID   -
Organism   Hydrogenophaga sp. UC242_53     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4144558..4155601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJI0_RS20820 (ACFJI0_20820) - 4144647..4147620 (-) 2974 Protein_4124 L-glutamate gamma-semialdehyde dehydrogenase -
  ACFJI0_RS20825 (ACFJI0_20825) - 4147727..4148197 (+) 471 WP_297395587.1 Lrp/AsnC ligand binding domain-containing protein -
  ACFJI0_RS20830 (ACFJI0_20830) - 4148267..4148815 (+) 549 WP_382322164.1 DUF3617 domain-containing protein -
  ACFJI0_RS20835 (ACFJI0_20835) - 4148799..4149518 (-) 720 WP_382153514.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJI0_RS20840 (ACFJI0_20840) pilT 4149558..4150601 (+) 1044 WP_291121788.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJI0_RS20845 (ACFJI0_20845) pilU 4150714..4151850 (+) 1137 WP_291120124.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJI0_RS20850 (ACFJI0_20850) - 4151956..4152857 (+) 902 Protein_4130 NAD(P)-dependent oxidoreductase -
  ACFJI0_RS20855 (ACFJI0_20855) - 4152965..4153627 (-) 663 WP_382153509.1 BON domain-containing protein -
  ACFJI0_RS20860 (ACFJI0_20860) - 4153624..4154244 (-) 621 WP_291120132.1 SIS domain-containing protein -
  ACFJI0_RS20865 (ACFJI0_20865) - 4154281..4154718 (-) 438 WP_382163836.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38148.77 Da        Isoelectric Point: 6.9313

>NTDB_id=1057951 ACFJI0_RS20840 WP_291121788.1 4149558..4150601(+) (pilT) [Hydrogenophaga sp. UC242_53]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLDHKQVHGMVYDIMNDSQRKQYEEFLECDFSFEIEGLAR
FRVNAFNHNRGAGAVFRTIPSKILTLEQLNAPKIFGDLALRPRGLVLVTGPTGSGKSTTLAGMVNHLNETEYGHILTVED
PVEFVHESKKCLVNQREVGPHTLSFSNALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKDMVRAMLSESLVAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSAIQTGNSVGMQTLDQN
LSELVKRNVISPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1057951 ACFJI0_RS20840 WP_291121788.1 4149558..4150601(+) (pilT) [Hydrogenophaga sp. UC242_53]
ATGGATATCACCCAACTGCTGGCTTTCAGCGTCAAGAACAAAGCATCCGATCTTCATCTCTCGGCCGGTCTTCCACCCAT
GATACGGGTGCATGGTGATGTGCGACGCATCAACGTCGAACCGCTGGACCACAAGCAGGTCCACGGCATGGTGTACGACA
TCATGAACGACAGCCAGCGCAAGCAGTACGAAGAGTTCCTGGAGTGCGACTTCTCGTTCGAGATCGAAGGCCTGGCCCGC
TTCCGCGTCAACGCCTTCAACCACAACCGCGGCGCTGGCGCGGTGTTCCGCACCATCCCGAGCAAGATCCTCACGCTGGA
GCAACTCAACGCACCCAAGATCTTCGGCGACCTCGCGCTGCGCCCGCGCGGCCTGGTGCTGGTGACCGGGCCCACCGGTT
CGGGCAAGTCCACCACGCTCGCCGGCATGGTCAACCACCTCAATGAAACCGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGGTGGAGTTCGTGCACGAGTCCAAGAAGTGCCTGGTCAACCAGCGCGAGGTGGGCCCGCACACGCTGAGTTTTTCGAA
CGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACGCGATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTGG
CGATGACCGCCGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCCAGCGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGAAGAGAAAGACATGGTGCGCGCGATGCTCTCCGAATCGCTGGTGGCGGTGATTTCGCAGAC
GCTGTGCAAGACCAAGGACGGCTCGGGCCGTGTCGCGGCGCACGAGATCATGCTGGGCACCAGCGCGATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAAATGTATTCGGCCATCCAGACCGGCAACAGCGTGGGCATGCAGACGCTGGACCAGAAC
CTCTCGGAGCTGGTCAAGCGCAATGTGATCAGCCCGGCGGAGGCGCGCGGCAAGGCGAAGATCCCCGAGAACTTCCCAGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

72.861

97.695

0.712
  pilT Legionella pneumophila strain ERS1305867

72.861

97.695

0.712
  pilT Neisseria meningitidis 8013

66.957

99.424

0.666
  pilT Neisseria gonorrhoeae MS11

66.667

99.424

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.862

98.271

0.657
  pilT Vibrio cholerae strain A1552

66.862

98.271

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment