Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFJI0_RS20845 Genome accession   NZ_CP170552
Coordinates   4150714..4151850 (+) Length   378 a.a.
NCBI ID   WP_291120124.1    Uniprot ID   -
Organism   Hydrogenophaga sp. UC242_53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4145714..4156850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJI0_RS20825 (ACFJI0_20825) - 4147727..4148197 (+) 471 WP_297395587.1 Lrp/AsnC ligand binding domain-containing protein -
  ACFJI0_RS20830 (ACFJI0_20830) - 4148267..4148815 (+) 549 WP_382322164.1 DUF3617 domain-containing protein -
  ACFJI0_RS20835 (ACFJI0_20835) - 4148799..4149518 (-) 720 WP_382153514.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJI0_RS20840 (ACFJI0_20840) pilT 4149558..4150601 (+) 1044 WP_291121788.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJI0_RS20845 (ACFJI0_20845) pilU 4150714..4151850 (+) 1137 WP_291120124.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJI0_RS20850 (ACFJI0_20850) - 4151956..4152857 (+) 902 Protein_4130 NAD(P)-dependent oxidoreductase -
  ACFJI0_RS20855 (ACFJI0_20855) - 4152965..4153627 (-) 663 WP_382153509.1 BON domain-containing protein -
  ACFJI0_RS20860 (ACFJI0_20860) - 4153624..4154244 (-) 621 WP_291120132.1 SIS domain-containing protein -
  ACFJI0_RS20865 (ACFJI0_20865) - 4154281..4154718 (-) 438 WP_382163836.1 YraN family protein -
  ACFJI0_RS20870 (ACFJI0_20870) rsmI 4154718..4155629 (+) 912 WP_382322165.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACFJI0_RS20875 (ACFJI0_20875) - 4155623..4155979 (-) 357 WP_382322166.1 hypothetical protein -
  ACFJI0_RS20880 (ACFJI0_20880) - 4156106..4156841 (+) 736 Protein_4136 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41823.26 Da        Isoelectric Point: 7.0165

>NTDB_id=1057952 ACFJI0_RS20845 WP_291120124.1 4150714..4151850(+) (pilU) [Hydrogenophaga sp. UC242_53]
MERDQASKFINDLLRLMLSRSGSDLFLTADFPPAIKVDGSVVKVSPQPLNATHTLALARAIMNDKQAAEFERTKECNFAI
SPPAIGRFRVNAFIQQGKVGMVLRTIPAVLPTIDKLGLPQVLKDVALSKRGLCILVGATGSGKSTSLAAMVDWRNENTHG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDREAMEHAIQFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRALVSQRLVPRQDNKGRHAAVEIMLNSPLISDLIFKGDVAEIKEIMKKSTQMG
MQTFDQALFNAFEANLITLEDALRNADSLNDLRLQIKLNSQRAKSLDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1057952 ACFJI0_RS20845 WP_291120124.1 4150714..4151850(+) (pilU) [Hydrogenophaga sp. UC242_53]
ATGGAACGCGACCAGGCATCCAAATTCATCAACGACCTGCTGCGCCTCATGCTCAGCCGCTCGGGCAGCGACCTGTTCCT
GACCGCCGATTTTCCGCCAGCGATCAAGGTCGACGGCTCGGTCGTCAAGGTGTCGCCGCAACCCCTGAACGCCACGCACA
CGCTGGCGCTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCCGAATTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGCCGGCCATCGGGCGCTTCCGCGTCAACGCCTTCATCCAGCAGGGCAAGGTCGGCATGGTGCTGCGCACGATTCC
CGCCGTGCTGCCCACCATCGACAAGCTCGGCCTGCCCCAGGTGCTCAAGGACGTGGCGCTCTCCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCGACCGGCTCGGGCAAGTCCACCTCGCTCGCGGCCATGGTGGACTGGCGCAACGAGAACACGCACGGC
CACATCATCACGGTGGAAGACCCGATCGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCCT
GGACACCGACAGCTGGGAGGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGATGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAAGCCATGGAGCACGCGATCCAGTTCTCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGTGCCCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCGCTGGTCTCCCAGCGCCTGGTGCCGCGCCAGGACAACAAGGGCCGGCACGCGGCGGTGGAGATCATGCTGAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGATGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCACCCAGATGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCAACGCCTTCGAGGCCAACCTCATCACGCTCGAAGACGCGCTGCGCAACGCCGA
TTCGCTCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCCAAGTCGCTGGACCTGGCAGCGGGCACCGAGC
ACCTCACCATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.929

96.825

0.59
  pilU Acinetobacter baylyi ADP1

55.153

94.974

0.524
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

43.62

89.153

0.389
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376
  pilT Acinetobacter baumannii strain A118

44.514

84.392

0.376
  pilT Acinetobacter baumannii D1279779

44.514

84.392

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.153

0.373
  pilT Legionella pneumophila strain ERS1305867

42.857

87.037

0.373
  pilT Legionella pneumophila strain Lp02

42.857

87.037

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365

Multiple sequence alignment