Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEQUB_RS13235 Genome accession   NZ_CP170451
Coordinates   2682923..2683966 (-) Length   347 a.a.
NCBI ID   WP_197332038.1    Uniprot ID   G3A174
Organism   Ralstonia syzygii strain Br19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2677923..2688966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQUB_RS13205 (ACEQUB_02692) - 2678462..2678998 (-) 537 WP_075463469.1 PilX N-terminal domain-containing pilus assembly protein -
  ACEQUB_RS13210 (ACEQUB_02694) - 2679002..2680023 (-) 1022 Protein_2593 PilW family protein -
  ACEQUB_RS13215 (ACEQUB_02695) pilV 2680020..2680610 (-) 591 WP_075463467.1 type IV pilus modification protein PilV -
  ACEQUB_RS13220 (ACEQUB_02696) - 2680607..2681095 (-) 489 WP_197332040.1 GspH/FimT family pseudopilin -
  ACEQUB_RS13225 (ACEQUB_02697) - 2681099..2681539 (-) 441 WP_197329727.1 type IV pilin protein -
  ACEQUB_RS13230 (ACEQUB_02698) pilU 2681734..2682870 (-) 1137 WP_197332039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEQUB_RS13235 (ACEQUB_02699) pilT 2682923..2683966 (-) 1044 WP_197332038.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEQUB_RS13240 (ACEQUB_02700) - 2684089..2684811 (+) 723 WP_197332328.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEQUB_RS13245 (ACEQUB_02701) proC 2684846..2685670 (+) 825 WP_197332037.1 pyrroline-5-carboxylate reductase -
  ACEQUB_RS13250 (ACEQUB_02702) - 2685683..2685961 (+) 279 WP_197332036.1 hypothetical protein -
  ACEQUB_RS13255 (ACEQUB_02703) ubiA 2685958..2686833 (-) 876 WP_197329723.1 4-hydroxybenzoate octaprenyltransferase -
  ACEQUB_RS13260 (ACEQUB_02704) - 2686906..2687406 (-) 501 WP_003271865.1 Dps family protein -
  ACEQUB_RS13265 (ACEQUB_02705) - 2687568..2687810 (-) 243 WP_013211540.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38152.82 Da        Isoelectric Point: 6.8706

>NTDB_id=1057518 ACEQUB_RS13235 WP_197332038.1 2682923..2683966(-) (pilT) [Ralstonia syzygii strain Br19]
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPALTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPSEEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1057518 ACEQUB_RS13235 WP_197332038.1 2682923..2683966(-) (pilT) [Ralstonia syzygii strain Br19]
ATGGACATCGCGCAGCTTCTGGCCTTCGCCGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCGCCGAT
GATCCGGATCCATGGCGACATGCGTCGTATCAACGTGCCAGCGCTCACGCACCAGGATGTTCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCCGACTTCTCGTTCGAGATCCCCGGGCTGTCCCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCGCCGCCGTGTTCCGGACGATTCCGTCCAAGGTGCTGACGCTGGA
AGACCTGAAGGCGCCGGCCGTCTTCGCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAATCACCGCAACGAAAGCGACCTGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAACTGGGGCCGCATACGCATTCGTTTGCCAA
CGCGCTGAAGTCGGCGCTGCGGGAAGATCCGGACGTGGTCCTGGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTGG
CACTGACGGCGGCCGAAACCGGCCACTTGGTGTTCGCCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGTCGGAAGAGAAGGACATGGTCCGCACCATGCTGTCCGAATCGCTGGAGGCGGTGATTTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATCTGCACGCCGGCCATCCGCCACCTGA
TCCGCGAGAACAAGATCTCGCAGATGTATTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGGCGGAGCTCATCAAGCGCTCGGCGATCAACTACGCCGACGCGCGCGCCATCGCCAAGAATCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3A174

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.884

99.424

0.715
  pilT Acinetobacter baumannii D1279779

71.884

99.424

0.715
  pilT Acinetobacter baumannii strain A118

71.884

99.424

0.715
  pilT Pseudomonas stutzeri DSM 10701

71.304

99.424

0.709
  pilT Pseudomonas aeruginosa PAK

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

70.145

99.424

0.697
  pilT Neisseria gonorrhoeae MS11

68.208

99.712

0.68
  pilT Neisseria meningitidis 8013

68.208

99.712

0.68
  pilT Legionella pneumophila strain Lp02

67.536

99.424

0.671
  pilT Legionella pneumophila strain ERS1305867

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.118

0.643
  pilT Vibrio cholerae strain A1552

66.172

97.118

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.578

99.712

0.504
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Vibrio cholerae strain A1552

42.687

96.542

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406

Multiple sequence alignment