Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEQUB_RS13230 Genome accession   NZ_CP170451
Coordinates   2681734..2682870 (-) Length   378 a.a.
NCBI ID   WP_197332039.1    Uniprot ID   G3A173
Organism   Ralstonia syzygii strain Br19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2676734..2687870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQUB_RS13205 (ACEQUB_02692) - 2678462..2678998 (-) 537 WP_075463469.1 PilX N-terminal domain-containing pilus assembly protein -
  ACEQUB_RS13210 (ACEQUB_02694) - 2679002..2680023 (-) 1022 Protein_2593 PilW family protein -
  ACEQUB_RS13215 (ACEQUB_02695) pilV 2680020..2680610 (-) 591 WP_075463467.1 type IV pilus modification protein PilV -
  ACEQUB_RS13220 (ACEQUB_02696) - 2680607..2681095 (-) 489 WP_197332040.1 GspH/FimT family pseudopilin -
  ACEQUB_RS13225 (ACEQUB_02697) - 2681099..2681539 (-) 441 WP_197329727.1 type IV pilin protein -
  ACEQUB_RS13230 (ACEQUB_02698) pilU 2681734..2682870 (-) 1137 WP_197332039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEQUB_RS13235 (ACEQUB_02699) pilT 2682923..2683966 (-) 1044 WP_197332038.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEQUB_RS13240 (ACEQUB_02700) - 2684089..2684811 (+) 723 WP_197332328.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEQUB_RS13245 (ACEQUB_02701) proC 2684846..2685670 (+) 825 WP_197332037.1 pyrroline-5-carboxylate reductase -
  ACEQUB_RS13250 (ACEQUB_02702) - 2685683..2685961 (+) 279 WP_197332036.1 hypothetical protein -
  ACEQUB_RS13255 (ACEQUB_02703) ubiA 2685958..2686833 (-) 876 WP_197329723.1 4-hydroxybenzoate octaprenyltransferase -
  ACEQUB_RS13260 (ACEQUB_02704) - 2686906..2687406 (-) 501 WP_003271865.1 Dps family protein -
  ACEQUB_RS13265 (ACEQUB_02705) - 2687568..2687810 (-) 243 WP_013211540.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42067.55 Da        Isoelectric Point: 7.0670

>NTDB_id=1057517 ACEQUB_RS13230 WP_197332039.1 2681734..2682870(-) (pilU) [Ralstonia syzygii strain Br19]
MLDREAATKYIHELLQLMVNSRGSDLFITSEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMNEKQLREFEGSFECNFA
IVSGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLPPILNEVAMSKRGLVIVVGATGSGKSTTLAAMVGYRNAHSYG
HIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANSS
NQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGRKGRAPAIEILLGTPLVADLIFKGEVHELKEVMKKSREQG
MVSFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1057517 ACEQUB_RS13230 WP_197332039.1 2681734..2682870(-) (pilU) [Ralstonia syzygii strain Br19]
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGACCTGTT
CATCACCTCGGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGTTGACCACCGTCC
AGGCGATGGGCCTGGTCAAGGCGATCATGAACGAGAAGCAGTTGCGCGAGTTCGAAGGCTCCTTCGAGTGCAACTTTGCC
ATCGTGTCCGGCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCATCAA
CACCAAGATCCCGACGCTCGGCGAACTGGATCTGCCGCCCATCCTCAACGAGGTCGCGATGAGCAAGCGCGGGCTCGTGA
TCGTGGTGGGGGCGACGGGCTCCGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTACGGC
CACATCATCACCATTGAAGACCCCGTGGAATACGTGCACGCACACCAGAACTGCGTCGTGACGCAGCGCGAGGTGGGCGT
GGATACGGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ATCGCGACACGATGGAGTACGCCATCCAGTACGCCGAAACCGGTCACTTGTGCCTGGCCACGCTGCACGCCAACAGCTCG
AACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAGCTGCTGATCGACCTGTCCTTGAACCT
GCGCGCCATGATTGCGCAGCGCCTGCTGCCGCGCAAAGGCAGGAAGGGCCGCGCGCCGGCGATCGAAATCCTGCTGGGCA
CGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGGTGCATGAGCTCAAGGAAGTCATGAAGAAGTCCCGCGAGCAGGGG
ATGGTCTCGTTCGACCAGGCGCTGTTCGAGCTCTATGAGGCGGACAAGATCACCTATGAGGACGCGCTGCGCAACGCAGA
CTCGCTGAACGATCTGCGTCTGCAGATCAAGCTGCACAGCAAGCGCGGCGGGCCGGTGGACCTGACGGCCGGCACCGAGC
ATCTGAACGTGATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3A173

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.606

93.386

0.585
  pilU Vibrio cholerae strain A1552

56.091

93.386

0.524
  pilU Acinetobacter baylyi ADP1

54.875

94.974

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Pseudomonas aeruginosa PAK

42.73

89.153

0.381
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Vibrio cholerae strain A1552

44.795

83.862

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.795

83.862

0.376
  pilT Acinetobacter baylyi ADP1

44.099

85.185

0.376
  pilT Pseudomonas stutzeri DSM 10701

42.136

89.153

0.376
  pilT Acinetobacter baumannii D1279779

43.26

84.392

0.365
  pilT Acinetobacter baumannii strain A118

43.26

84.392

0.365
  pilT Acinetobacter nosocomialis M2

43.26

84.392

0.365

Multiple sequence alignment