Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB5G97_RS03170 Genome accession   NZ_CP170371
Coordinates   639782..640816 (+) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain Aer12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 634782..645816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5G97_RS03140 (AB5G97_03140) - 635448..636644 (+) 1197 WP_005348874.1 NupC/NupG family nucleoside CNT transporter -
  AB5G97_RS03145 (AB5G97_03145) - 636746..637165 (-) 420 WP_376869344.1 DUF4426 domain-containing protein -
  AB5G97_RS03150 (AB5G97_03150) yggU 637209..637511 (-) 303 WP_179902587.1 DUF167 family protein YggU -
  AB5G97_RS03155 (AB5G97_03155) - 637511..638062 (-) 552 WP_115521283.1 YggT family protein -
  AB5G97_RS03160 (AB5G97_03160) proC 638074..638898 (-) 825 WP_376869345.1 pyrroline-5-carboxylate reductase -
  AB5G97_RS03165 (AB5G97_03165) - 639041..639742 (-) 702 WP_376869346.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB5G97_RS03170 (AB5G97_03170) pilT 639782..640816 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  AB5G97_RS03175 (AB5G97_03175) pilU 640845..641954 (+) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  AB5G97_RS03180 (AB5G97_03180) yaaA 642002..642775 (+) 774 WP_204384342.1 peroxide stress protein YaaA -
  AB5G97_RS03185 (AB5G97_03185) - 642970..643395 (+) 426 WP_005340360.1 hypothetical protein -
  AB5G97_RS03190 (AB5G97_03190) - 643756..644247 (-) 492 WP_376869347.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  AB5G97_RS03195 (AB5G97_03195) arfB 644278..644694 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  AB5G97_RS03200 (AB5G97_03200) glnK 644896..645234 (+) 339 WP_005341406.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=1056355 AB5G97_RS03170 WP_005340354.1 639782..640816(+) (pilT) [Aeromonas veronii strain Aer12]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1056355 AB5G97_RS03170 WP_005340354.1 639782..640816(+) (pilT) [Aeromonas veronii strain Aer12]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTACCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAAGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTTAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACGGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGTGGTCTGGTGCTGGTGACCGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCGGCCATGGTCAACTACATTAACGAGAACTTCCATCACCACATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGAACAAACGCTGTCTGGTGAACCAGCGGGAAGTGCACCGGGATACCAAGAGCTTTAGCAA
CGCTCTGCGCTCGGCCCTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACTGCTGCCGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATCC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTTAGTCGTGGCGTGGTGGCATCCCTTGATGCCAAGGCCAAGGCCGTCGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment