Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB5G97_RS03175 Genome accession   NZ_CP170371
Coordinates   640845..641954 (+) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain Aer12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 635845..646954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5G97_RS03145 (AB5G97_03145) - 636746..637165 (-) 420 WP_376869344.1 DUF4426 domain-containing protein -
  AB5G97_RS03150 (AB5G97_03150) yggU 637209..637511 (-) 303 WP_179902587.1 DUF167 family protein YggU -
  AB5G97_RS03155 (AB5G97_03155) - 637511..638062 (-) 552 WP_115521283.1 YggT family protein -
  AB5G97_RS03160 (AB5G97_03160) proC 638074..638898 (-) 825 WP_376869345.1 pyrroline-5-carboxylate reductase -
  AB5G97_RS03165 (AB5G97_03165) - 639041..639742 (-) 702 WP_376869346.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB5G97_RS03170 (AB5G97_03170) pilT 639782..640816 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  AB5G97_RS03175 (AB5G97_03175) pilU 640845..641954 (+) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  AB5G97_RS03180 (AB5G97_03180) yaaA 642002..642775 (+) 774 WP_204384342.1 peroxide stress protein YaaA -
  AB5G97_RS03185 (AB5G97_03185) - 642970..643395 (+) 426 WP_005340360.1 hypothetical protein -
  AB5G97_RS03190 (AB5G97_03190) - 643756..644247 (-) 492 WP_376869347.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  AB5G97_RS03195 (AB5G97_03195) arfB 644278..644694 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  AB5G97_RS03200 (AB5G97_03200) glnK 644896..645234 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  AB5G97_RS03205 (AB5G97_03205) - 645493..646500 (+) 1008 WP_005357960.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=1056356 AB5G97_RS03175 WP_005340356.1 640845..641954(+) (pilU) [Aeromonas veronii strain Aer12]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1056356 AB5G97_RS03175 WP_005340356.1 640845..641954(+) (pilU) [Aeromonas veronii strain Aer12]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGTGGGGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCGCTTGGTCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCGGCGATGATTGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGTAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAATTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAATCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCGACCGAGCTGGGTATGCAGACCTTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGTACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36

Multiple sequence alignment