Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LMH68_RS10240 Genome accession   NZ_CP170175
Coordinates   2197031..2198140 (+) Length   369 a.a.
NCBI ID   WP_229648165.1    Uniprot ID   -
Organism   Vibrio sp. F13 strain 10N.222.55.B3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2192031..2203140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH68_RS10205 (LMH68_010205) - 2192289..2192888 (-) 600 WP_017062247.1 XTP/dITP diphosphatase -
  LMH68_RS10210 (LMH68_010210) - 2192937..2193368 (-) 432 WP_017062246.1 DUF4426 domain-containing protein -
  LMH68_RS10215 (LMH68_010215) yggU 2193397..2193687 (-) 291 WP_102427900.1 DUF167 family protein YggU -
  LMH68_RS10220 (LMH68_010220) - 2193717..2194274 (-) 558 WP_017067798.1 YggT family protein -
  LMH68_RS10225 (LMH68_010225) proC 2194351..2195169 (-) 819 WP_017063196.1 pyrroline-5-carboxylate reductase -
  LMH68_RS10230 (LMH68_010230) - 2195248..2195952 (-) 705 WP_017062243.1 YggS family pyridoxal phosphate-dependent enzyme -
  LMH68_RS10235 (LMH68_010235) pilT 2195979..2197016 (+) 1038 WP_017062242.1 type IV pilus twitching motility protein PilT Machinery gene
  LMH68_RS10240 (LMH68_010240) pilU 2197031..2198140 (+) 1110 WP_229648165.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LMH68_RS10245 (LMH68_010245) - 2198215..2198352 (-) 138 WP_229648164.1 hypothetical protein -
  LMH68_RS10250 (LMH68_010250) ruvX 2198452..2198874 (-) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  LMH68_RS10255 (LMH68_010255) - 2198935..2199498 (-) 564 WP_017062239.1 YqgE/AlgH family protein -
  LMH68_RS10260 (LMH68_010260) gshB 2199615..2200565 (-) 951 WP_004735482.1 glutathione synthase -
  LMH68_RS10265 (LMH68_010265) rsmE 2200575..2201306 (-) 732 WP_017062238.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LMH68_RS10270 (LMH68_010270) - 2201460..2202191 (-) 732 WP_017063198.1 endonuclease -
  LMH68_RS10275 (LMH68_010275) - 2202288..2202788 (-) 501 WP_017062236.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41395.30 Da        Isoelectric Point: 5.8713

>NTDB_id=1055447 LMH68_RS10240 WP_229648165.1 2197031..2198140(+) (pilU) [Vibrio sp. F13 strain 10N.222.55.B3]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVAQLLDAMMEQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVIAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALASVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1055447 LMH68_RS10240 WP_229648165.1 2197031..2198140(+) (pilU) [Vibrio sp. F13 strain 10N.222.55.B3]
ATGGAATTGAATCAAATCCTTGAGGGAATGCTTTCTCAAAAGGCATCGGATCTTTATATCACGGTTGATGCGCCAATCCT
TTTTCGTGTGGATGGTGAATTACGCCCCCAAGGAGAGAAGCTGAATGCGGCTCAAGTTGCTCAATTACTTGATGCGATGA
TGGAACAAGACCGACGTGATGAATATCAGCAAACGCGTGAGGCTAATTTTGCGATTGTGCGTGACTTTGGTCGTTTTCGT
GTCAGTGCATTCTTTCAGCGAGAGCTACCTGGAGCGGTCATTCGACGTATCGAGACTAACATCCCAACCTTTGAGCAACT
AAAGCTTCCTGATGTACTACAGGACCTATCAATAGCTAAGCGCGGACTTGTGCTGGTGGTTGGTGCGACAGGCTCTGGTA
AGTCGACCTCAATGGCAGCAATGACAGGCTATCGCAACACCAATCGCTCGGGACATATCTTGACGGTTGAAGACCCGATT
GAATTTGTGCATGAACATAAAAAGTGCATCGTGACACAGCGTGAAGTTGGACTCGACACCGAGAGCTATGAAGTCGCGCT
TAAGAACTCGCTACGCCAAGCACCAGACATGATCTTGATTGGCGAAATCCGTAGCCGTGAGACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCAACCTTGCACGCCAATAACGCTAACCAAGCGCTAGAGCGTATTCTTCAT
TTGGTGCCGAAAGAGCAGAAAGAACAGTTCTTGTTTGACCTATCGATGAACCTGCGTGGTGTCGTCGCTCAGCAACTCAT
TCGCGACAAGAATGGCAGCGGGCGTCATGGTGTATTCGAGATTCTGCTGAATAGCCCTCGTGTTTCTGACTTGATTCGTC
GTGGTGAACTGCACGAACTGAAAGCAACCATGGCTAAATCGAAAGAGATAGGTATGCAGACCTTTGACCAAGCTTTGTAT
GATTTAGTGATAGCAGGCAAGATCAGCGAAGAGGATGCGTTCCACAGTGCCGATTCTGCTAATGACTTGCGCTTAATGCT
CAAAACTAGACGTGGTGATGACGACTACGGAACTGGGGCGTTGGCTAGTGTGAAGATAGATATGGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

99.729

0.84
  pilU Pseudomonas stutzeri DSM 10701

58.286

94.851

0.553
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Acinetobacter baumannii D1279779

41.298

91.87

0.379
  pilT Acinetobacter baumannii strain A118

41.298

91.87

0.379
  pilT Acinetobacter nosocomialis M2

41.298

91.87

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Acinetobacter baylyi ADP1

41.088

89.702

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363

Multiple sequence alignment