Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LMH68_RS10235 Genome accession   NZ_CP170175
Coordinates   2195979..2197016 (+) Length   345 a.a.
NCBI ID   WP_017062242.1    Uniprot ID   A0A4U2ACB1
Organism   Vibrio sp. F13 strain 10N.222.55.B3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2190979..2202016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH68_RS10200 (LMH68_010200) hemW 2191102..2192289 (-) 1188 WP_229648166.1 radical SAM family heme chaperone HemW -
  LMH68_RS10205 (LMH68_010205) - 2192289..2192888 (-) 600 WP_017062247.1 XTP/dITP diphosphatase -
  LMH68_RS10210 (LMH68_010210) - 2192937..2193368 (-) 432 WP_017062246.1 DUF4426 domain-containing protein -
  LMH68_RS10215 (LMH68_010215) yggU 2193397..2193687 (-) 291 WP_102427900.1 DUF167 family protein YggU -
  LMH68_RS10220 (LMH68_010220) - 2193717..2194274 (-) 558 WP_017067798.1 YggT family protein -
  LMH68_RS10225 (LMH68_010225) proC 2194351..2195169 (-) 819 WP_017063196.1 pyrroline-5-carboxylate reductase -
  LMH68_RS10230 (LMH68_010230) - 2195248..2195952 (-) 705 WP_017062243.1 YggS family pyridoxal phosphate-dependent enzyme -
  LMH68_RS10235 (LMH68_010235) pilT 2195979..2197016 (+) 1038 WP_017062242.1 type IV pilus twitching motility protein PilT Machinery gene
  LMH68_RS10240 (LMH68_010240) pilU 2197031..2198140 (+) 1110 WP_229648165.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LMH68_RS10245 (LMH68_010245) - 2198215..2198352 (-) 138 WP_229648164.1 hypothetical protein -
  LMH68_RS10250 (LMH68_010250) ruvX 2198452..2198874 (-) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  LMH68_RS10255 (LMH68_010255) - 2198935..2199498 (-) 564 WP_017062239.1 YqgE/AlgH family protein -
  LMH68_RS10260 (LMH68_010260) gshB 2199615..2200565 (-) 951 WP_004735482.1 glutathione synthase -
  LMH68_RS10265 (LMH68_010265) rsmE 2200575..2201306 (-) 732 WP_017062238.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38257.78 Da        Isoelectric Point: 6.0507

>NTDB_id=1055446 LMH68_RS10235 WP_017062242.1 2195979..2197016(+) (pilT) [Vibrio sp. F13 strain 10N.222.55.B3]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLDQLGAPEIFERIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1055446 LMH68_RS10235 WP_017062242.1 2195979..2197016(+) (pilT) [Vibrio sp. F13 strain 10N.222.55.B3]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATCCCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGCGATTCACAACGCGGCGAGTTTGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGC
TTTCGTGTTAATGCGTTTAACCAAGCCCGTGGCTGCTCAGCTGTCTTTCGAACTATCCCTGTCGAAATCCCGACTTTAGA
TCAGTTAGGTGCACCTGAGATCTTCGAAAGGATTGCTAACTACGAAAAAGGCTTAGTGCTCGTTACTGGTCCCACTGGCT
CTGGTAAATCAACGACTCTTGCGGCGATGGTTGATTACGTAAATCGCAACCACAATAAGCATATTCTGACGATTGAAGAC
CCGATTGAATTCGTTCACACCAATAATAAATGCCTAGTCAACCAGCGTGAAGTTCACCGTGATACTCACAGTTTTAAAGC
GGCACTGCGCAGTGCCTTACGTGAAGACCCAGACGTGATTCTGGTTGGTGAACTTCGTGACCAAGAGACGATTAGCTTAG
CGTTAACCGCAGCAGAAACGGGTCACTTGGTCTTTGGTACTCTGCACACCAGTTCTGCGGCTAAAACGATCGACCGTATT
ATCGATGTGTTCCCAGGTAGCGATAAAGATATGGTTCGTTCGATGTTGTCTGAATCACTGCGCTCGGTGATAGCCCAGAA
GCTGCTAAAGCGTGTTGGTGGTGGTCGTGTGGCGTGTCATGAAATCATGATGGCAACACCGGCGATCAGAAACTTGATTC
GTGAAGATAAGGTCGCTCAGATGTACTCGATCATTCAAACGGGCGCAGCGCACGGCATGCAGACCATGGAACAAAATGCG
AAGCAGCTGATGGCGCAGGGCTTGGTTGATTCGGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U2ACB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.988

96.232

0.693
  pilT Acinetobacter nosocomialis M2

71.687

96.232

0.69
  pilT Pseudomonas aeruginosa PAK

69.118

98.551

0.681
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.319

100

0.623
  pilT Neisseria gonorrhoeae MS11

62.029

100

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.812

0.391
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment