Detailed information NTG1055060

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ACE38K_RS14235
• Genome accession: NZ_CP170101
• Coordinates: 2893232..2894212 (-)
• Length: 326 a.a.
• NCBI ID: WP_012767756.1
• Uniprot ID: A0AAX0KGR9
• Organism: Escherichia coli BL21
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2888232..2899212

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACE38K_RS14205	yggI	2888887..2889384 (+)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-
ACE38K_RS14210	endA	2889479..2890186 (+)	708	WP_000286500.1	deoxyribonuclease I	-
ACE38K_RS14215	rsmE	2890266..2890997 (+)	732	WP_001300912.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ACE38K_RS14220	gshB	2891010..2891960 (+)	951	WP_000593273.1	glutathione synthase	-
ACE38K_RS14225	yqgE	2892069..2892632 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
ACE38K_RS14230	ruvX	2892632..2893048 (+)	417	WP_000017105.1	Holliday junction resolvase RuvX	-
ACE38K_RS14235	pilT	2893232..2894212 (-)	981	WP_012767756.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACE38K_RS14240	yggS	2894230..2894934 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
ACE38K_RS14245	yggT	2894952..2895518 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
ACE38K_RS14250	yggU	2895515..2895805 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
ACE38K_RS14255	rdgB	2895813..2896406 (+)	594	WP_001174737.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
ACE38K_RS14260	hemW	2896399..2897535 (+)	1137	WP_000239975.1	radical SAM family heme chaperone HemW	-
ACE38K_RS14265	yggM	2897600..2898607 (-)	1008	WP_000745230.1	DUF1202 family protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35980.13 Da
• Isoelectric Point: 6.0804

>NTDB_id=1055060 ACE38K_RS14235 WP_012767756.1 2893232..2894212(-) (pilT) [Escherichia coli BL21]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQSVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1055060 ACE38K_RS14235 WP_012767756.1 2893232..2894212(-) (pilT) [Escherichia coli BL21]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGTCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.453	97.546	0.414
pilU	Vibrio cholerae strain A1552	40.062	98.773	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	36.061	100	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362