Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEVBK_RS02250 Genome accession   NZ_CP169531
Coordinates   457373..458407 (+) Length   344 a.a.
NCBI ID   WP_375056997.1    Uniprot ID   -
Organism   Zobellella sp. DQSA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452373..463407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEVBK_RS02215 (ACEVBK_02215) hemW 452662..453807 (-) 1146 WP_375056990.1 radical SAM family heme chaperone HemW -
  ACEVBK_RS02220 (ACEVBK_02220) rdgB 453807..454406 (-) 600 WP_375056991.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  ACEVBK_RS02225 (ACEVBK_02225) - 454503..454916 (-) 414 WP_375056992.1 DUF4426 domain-containing protein -
  ACEVBK_RS02230 (ACEVBK_02230) yggU 454952..455263 (-) 312 WP_375056993.1 DUF167 family protein YggU -
  ACEVBK_RS02235 (ACEVBK_02235) - 455251..455802 (-) 552 WP_375056994.1 YggT family protein -
  ACEVBK_RS02240 (ACEVBK_02240) proC 455812..456636 (-) 825 WP_375056995.1 pyrroline-5-carboxylate reductase -
  ACEVBK_RS02245 (ACEVBK_02245) - 456647..457339 (-) 693 WP_375056996.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEVBK_RS02250 (ACEVBK_02250) pilT 457373..458407 (+) 1035 WP_375056997.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEVBK_RS02255 (ACEVBK_02255) pilU 458475..459584 (+) 1110 WP_375056998.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEVBK_RS02260 (ACEVBK_02260) yaaA 459594..460367 (+) 774 WP_375056999.1 peroxide stress protein YaaA -
  ACEVBK_RS02265 (ACEVBK_02265) - 460483..460632 (-) 150 WP_375057000.1 hypothetical protein -
  ACEVBK_RS02270 (ACEVBK_02270) srmB 460692..461987 (-) 1296 WP_375057001.1 ATP-dependent RNA helicase SrmB -
  ACEVBK_RS02275 (ACEVBK_02275) - 462058..462765 (+) 708 WP_375058498.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38144.96 Da        Isoelectric Point: 7.3952

>NTDB_id=1051805 ACEVBK_RS02250 WP_375056997.1 457373..458407(+) (pilT) [Zobellella sp. DQSA1]
MDITELLAFSVKHNASDLHLSAGVPPLIRVDGEVRKINLPVQEHREVHRLVYDIMNDRQRKELEENLEIDFSFELPGLAR
FRVNAYHQARGAAAVFRIIPSEVRSLEQLGAPEIFRRIAEYPRGLVLVTGPAGSGKSTTLAAMIDHVNESFNKHILTIED
PIEFVHGNKKCLLNQREVHRDTHSFSNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKGMVRSMLSESLRAVISQTLLKKVNGGRIAAHEIMLGTPAIRNLIREDKVAQMYSVIQTGMAHGMQTMDQCL
KLLVSSGRVAPVDARTKAVDPQGI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1051805 ACEVBK_RS02250 WP_375056997.1 457373..458407(+) (pilT) [Zobellella sp. DQSA1]
ATGGATATTACGGAACTTTTGGCGTTTAGTGTAAAGCATAATGCATCGGATCTGCACCTGTCGGCGGGAGTGCCGCCCTT
GATCCGGGTGGACGGTGAGGTACGCAAGATCAATTTACCGGTGCAGGAACACCGGGAAGTACACCGGCTGGTATATGACA
TCATGAACGATCGCCAGCGCAAGGAGTTGGAGGAAAACCTAGAGATCGACTTTTCCTTCGAACTGCCGGGCCTGGCCCGT
TTCCGGGTGAATGCCTACCATCAAGCTCGCGGCGCGGCGGCGGTGTTCCGGATTATCCCGAGCGAAGTCAGAAGCCTGGA
GCAGTTGGGAGCCCCCGAGATCTTCCGCCGCATTGCCGAATATCCGCGCGGTCTGGTGCTGGTAACGGGGCCTGCCGGCT
CGGGTAAATCCACCACCCTGGCGGCGATGATCGATCATGTCAACGAGAGCTTCAACAAGCATATCCTCACCATTGAGGAT
CCCATCGAATTCGTGCACGGTAACAAGAAATGTCTGCTCAACCAGCGGGAAGTGCACCGGGATACCCACTCCTTCAGCAA
CGCGTTGCGTTCGGCGCTCCGGGAAGACCCCGATATTATCCTGGTAGGCGAACTGCGGGATCTGGAAACCATTCGCCTGG
CACTGACCGCCGCCGAAACCGGCCATCTGGTGTTCGGTACCCTGCACACCTCCTCGGCGGCCAAGACCATAGATCGTATT
ATCGATGTCTTCCCCGGTGCCGAGAAAGGCATGGTACGTTCCATGCTGTCCGAGTCGCTGCGGGCGGTGATCTCCCAGAC
CCTGCTCAAGAAGGTGAACGGTGGCCGTATTGCCGCCCACGAGATCATGCTGGGTACCCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCGGTGATCCAGACCGGCATGGCCCACGGCATGCAAACCATGGATCAGTGCCTC
AAGCTGCTGGTCAGCAGCGGCAGGGTGGCGCCGGTGGATGCCCGGACCAAGGCGGTAGACCCGCAAGGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.023

99.419

0.756
  pilT Acinetobacter baumannii D1279779

75.731

99.419

0.753
  pilT Acinetobacter baumannii strain A118

75.731

99.419

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Pseudomonas aeruginosa PAK

74.854

99.419

0.744
  pilT Pseudomonas stutzeri DSM 10701

74.854

99.419

0.744
  pilT Acinetobacter baylyi ADP1

74.561

99.419

0.741
  pilT Legionella pneumophila strain Lp02

71.765

98.837

0.709
  pilT Legionella pneumophila strain ERS1305867

71.765

98.837

0.709
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.729

99.709

0.506
  pilU Vibrio cholerae strain A1552

40.653

97.965

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387

Multiple sequence alignment