Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEVBK_RS02255 Genome accession   NZ_CP169531
Coordinates   458475..459584 (+) Length   369 a.a.
NCBI ID   WP_375056998.1    Uniprot ID   -
Organism   Zobellella sp. DQSA1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453475..464584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEVBK_RS02220 (ACEVBK_02220) rdgB 453807..454406 (-) 600 WP_375056991.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  ACEVBK_RS02225 (ACEVBK_02225) - 454503..454916 (-) 414 WP_375056992.1 DUF4426 domain-containing protein -
  ACEVBK_RS02230 (ACEVBK_02230) yggU 454952..455263 (-) 312 WP_375056993.1 DUF167 family protein YggU -
  ACEVBK_RS02235 (ACEVBK_02235) - 455251..455802 (-) 552 WP_375056994.1 YggT family protein -
  ACEVBK_RS02240 (ACEVBK_02240) proC 455812..456636 (-) 825 WP_375056995.1 pyrroline-5-carboxylate reductase -
  ACEVBK_RS02245 (ACEVBK_02245) - 456647..457339 (-) 693 WP_375056996.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEVBK_RS02250 (ACEVBK_02250) pilT 457373..458407 (+) 1035 WP_375056997.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEVBK_RS02255 (ACEVBK_02255) pilU 458475..459584 (+) 1110 WP_375056998.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEVBK_RS02260 (ACEVBK_02260) yaaA 459594..460367 (+) 774 WP_375056999.1 peroxide stress protein YaaA -
  ACEVBK_RS02265 (ACEVBK_02265) - 460483..460632 (-) 150 WP_375057000.1 hypothetical protein -
  ACEVBK_RS02270 (ACEVBK_02270) srmB 460692..461987 (-) 1296 WP_375057001.1 ATP-dependent RNA helicase SrmB -
  ACEVBK_RS02275 (ACEVBK_02275) - 462058..462765 (+) 708 WP_375058498.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACEVBK_RS02280 (ACEVBK_02280) brnQ 462918..464234 (+) 1317 WP_375057002.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40780.83 Da        Isoelectric Point: 6.5030

>NTDB_id=1051806 ACEVBK_RS02255 WP_375056998.1 458475..459584(+) (pilU) [Zobellella sp. DQSA1]
MELTPLLNAMLAQQASDMYISAGIPPTVKVQGRLLPVGADPLTAAQSLALIESCMNESWRLRFHQHKEANFAMTEANLGR
FRVSAFWQQEKAGMVIRRIESHIPTFEELYLPDTLREVAMTKRGLVLLVGGTGTGKSTTQAAMIGHRNRHAGGHILTIED
PVEYVHQHGQSIVTQREVGIDTESFGAALKSALRQAPDVIVIGEIRSEETMGFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHRQLLFDLSFNLKAVVAQQLIPSLDGIQRRGAFEILLNTPLVADLIRQGEMHRLKDIMAKSRELGMQTFDQA
LFDLYTRQHIGYSEALAHADSPNDLRLMIKLHGGGELDAGLLDNVTIES

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1051806 ACEVBK_RS02255 WP_375056998.1 458475..459584(+) (pilU) [Zobellella sp. DQSA1]
ATGGAATTGACGCCTTTATTGAATGCCATGCTGGCACAGCAGGCATCGGATATGTATATATCGGCGGGCATTCCGCCCAC
CGTCAAGGTGCAGGGCCGGCTGCTGCCGGTTGGTGCGGATCCACTCACCGCGGCCCAGTCTTTGGCTTTGATTGAATCTT
GCATGAATGAGAGCTGGCGTCTCCGGTTTCATCAACATAAAGAAGCCAACTTCGCCATGACCGAAGCCAACCTCGGCCGT
TTCCGGGTCAGTGCCTTCTGGCAGCAGGAAAAGGCGGGCATGGTGATACGGCGTATCGAGAGCCATATTCCCACCTTTGA
AGAGCTGTATCTGCCCGACACCTTGCGTGAAGTGGCCATGACCAAGCGCGGTCTGGTGCTGTTGGTGGGCGGAACCGGGA
CCGGCAAGTCCACCACTCAGGCGGCGATGATCGGCCACCGCAACCGCCATGCCGGCGGACATATCCTTACCATCGAGGAC
CCGGTGGAATACGTCCACCAGCATGGCCAGTCCATCGTGACCCAGCGGGAGGTGGGCATCGACACCGAGTCGTTCGGTGC
CGCCCTGAAAAGCGCCCTGCGCCAGGCGCCGGATGTGATCGTCATCGGCGAGATCCGGTCGGAAGAAACCATGGGCTTCG
CCCTGTCCTTTGCCGAAACCGGCCATCTGTGCATGGCTACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATT
ATGCATCTGGTGCCGGAAAGCAAGCACCGTCAATTGCTGTTCGATCTGTCGTTCAACCTTAAGGCGGTCGTGGCCCAGCA
GCTGATTCCCTCCCTCGATGGCATTCAGCGCCGGGGTGCCTTCGAGATCCTGCTCAATACCCCGCTGGTGGCGGACCTGA
TCCGCCAGGGCGAGATGCATCGGCTGAAGGACATCATGGCCAAATCTCGTGAGTTGGGTATGCAGACCTTCGACCAGGCG
TTGTTTGACCTCTACACCCGGCAGCATATAGGCTACTCCGAAGCTCTGGCCCATGCCGACTCGCCCAACGATCTGCGGTT
GATGATCAAACTGCACGGCGGCGGTGAACTGGATGCCGGCCTGCTCGATAACGTGACGATAGAATCTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.424

99.729

0.583
  pilU Pseudomonas stutzeri DSM 10701

58.743

99.187

0.583
  pilU Acinetobacter baylyi ADP1

58.074

95.664

0.556
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

93.496

0.407
  pilT Legionella pneumophila strain Lp02

41.176

92.141

0.379
  pilT Legionella pneumophila strain ERS1305867

41.176

92.141

0.379
  pilT Pseudomonas aeruginosa PAK

40.29

93.496

0.377
  pilT Acinetobacter baylyi ADP1

40.058

92.683

0.371
  pilT Neisseria gonorrhoeae MS11

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.284

88.618

0.366
  pilT Vibrio cholerae strain A1552

41.284

88.618

0.366
  pilT Pseudomonas stutzeri DSM 10701

39.13

93.496

0.366

Multiple sequence alignment