Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEY19_RS16440 Genome accession   NZ_CP169392
Coordinates   3450689..3451711 (+) Length   340 a.a.
NCBI ID   WP_374930080.1    Uniprot ID   -
Organism   Serratia marcescens strain IFSMLMEK1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3445689..3456711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY19_RS16405 (ACEY19_16405) - 3445902..3446336 (+) 435 WP_019456096.1 DUF29 domain-containing protein -
  ACEY19_RS16410 (ACEY19_16410) hemW 3446375..3447517 (-) 1143 WP_311265868.1 radical SAM family heme chaperone HemW -
  ACEY19_RS16415 (ACEY19_16415) - 3447510..3448103 (-) 594 WP_235363067.1 XTP/dITP diphosphatase -
  ACEY19_RS16420 (ACEY19_16420) yggU 3448244..3448537 (-) 294 WP_016930091.1 DUF167 family protein YggU -
  ACEY19_RS16425 (ACEY19_16425) - 3448534..3449088 (-) 555 WP_033632091.1 YggT family protein -
  ACEY19_RS16430 (ACEY19_16430) proC 3449120..3449941 (-) 822 WP_082997328.1 pyrroline-5-carboxylate reductase -
  ACEY19_RS16435 (ACEY19_16435) - 3449961..3450668 (-) 708 WP_015379048.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEY19_RS16440 (ACEY19_16440) pilT 3450689..3451711 (+) 1023 WP_374930080.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEY19_RS16445 (ACEY19_16445) ruvX 3451708..3452130 (-) 423 WP_033632095.1 Holliday junction resolvase RuvX -
  ACEY19_RS16450 (ACEY19_16450) - 3452130..3452693 (-) 564 WP_004937452.1 YqgE/AlgH family protein -
  ACEY19_RS16455 (ACEY19_16455) gshB 3452839..3453789 (-) 951 WP_033632096.1 glutathione synthase -
  ACEY19_RS16460 (ACEY19_16460) rsmE 3453800..3454531 (-) 732 WP_033632097.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACEY19_RS16465 (ACEY19_16465) endA 3454601..3455296 (-) 696 WP_015379043.1 deoxyribonuclease I -
  ACEY19_RS16470 (ACEY19_16470) - 3455398..3455910 (-) 513 WP_004937462.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36358.58 Da        Isoelectric Point: 7.1072

>NTDB_id=1051304 ACEY19_RS16440 WP_374930080.1 3450689..3451711(+) (pilT) [Serratia marcescens strain IFSMLMEK1]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGVSLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHHSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGECAAEGTGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1051304 ACEY19_RS16440 WP_374930080.1 3450689..3451711(+) (pilT) [Serratia marcescens strain IFSMLMEK1]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCATTGGCGGCGGCGCCAACGCTGACGGCGCAGGGCGCGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACAGGCCGAGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGAGTTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCAGCCATCGTCCCGGCGTTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAATTCCTGCATCACAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGTTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCTGGCGGCGGACGCGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCGGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGTGAGTGCGCGGCGGAGGGAACGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Neisseria gonorrhoeae MS11

49.702

98.824

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Legionella pneumophila strain ERS1305867

48.955

98.529

0.482
  pilT Legionella pneumophila strain Lp02

48.955

98.529

0.482
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Pseudomonas stutzeri DSM 10701

47.917

98.824

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.456
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.279

99.118

0.379

Multiple sequence alignment