Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEPPP_RS08850 Genome accession   NZ_CP169217
Coordinates   2011945..2012982 (+) Length   345 a.a.
NCBI ID   WP_374090707.1    Uniprot ID   -
Organism   Methylomicrobium lacus strain 22M6SE5-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2006945..2017982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPPP_RS08825 (ACEPPP_08825) - 2007455..2009113 (-) 1659 WP_374090701.1 multicopper oxidase domain-containing protein -
  ACEPPP_RS08830 (ACEPPP_08830) tusD 2009259..2009648 (+) 390 WP_374090702.1 sulfurtransferase complex subunit TusD -
  ACEPPP_RS08835 (ACEPPP_08835) tusC 2009655..2010011 (+) 357 WP_374090703.1 sulfurtransferase complex subunit TusC -
  ACEPPP_RS08840 (ACEPPP_08840) - 2010434..2011114 (+) 681 WP_374090704.1 hypothetical protein -
  ACEPPP_RS08845 (ACEPPP_08845) - 2011132..2011821 (-) 690 WP_374090705.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEPPP_RS08850 (ACEPPP_08850) pilT 2011945..2012982 (+) 1038 WP_374090707.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEPPP_RS08855 (ACEPPP_08855) pilU 2013005..2014138 (+) 1134 WP_374090708.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEPPP_RS08860 (ACEPPP_08860) panD 2014237..2014623 (-) 387 WP_374090709.1 aspartate 1-decarboxylase -
  ACEPPP_RS08865 (ACEPPP_08865) panC 2014790..2015632 (-) 843 WP_374090710.1 pantoate--beta-alanine ligase -
  ACEPPP_RS08870 (ACEPPP_08870) panB 2015635..2016426 (-) 792 WP_374090711.1 3-methyl-2-oxobutanoate hydroxymethyltransferase -
  ACEPPP_RS08875 (ACEPPP_08875) folK 2016428..2016931 (-) 504 WP_374090712.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38299.06 Da        Isoelectric Point: 7.3187

>NTDB_id=1049851 ACEPPP_RS08850 WP_374090707.1 2011945..2012982(+) (pilT) [Methylomicrobium lacus strain 22M6SE5-12]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDIRRINVPPLDHKAVHSLMYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQERGAAGVFRTIPSKVLSLEDLKAPKFFEEVTRKPRGLVLVTGPTGSGKSTTLAAMINHINTNDYAHILTVED
PIEFVHESQKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTAAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGMITAKTAATRAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1049851 ACEPPP_RS08850 WP_374090707.1 2011945..2012982(+) (pilT) [Methylomicrobium lacus strain 22M6SE5-12]
ATGGATATTGCCGAGTTACTGGCTTTTTCGGTCAAAAACAATGCGTCGGATTTACATTTATCGGCAGGGCTGCCGCCGAT
GATTCGGGTGGACGGCGATATTCGCCGGATCAATGTGCCGCCTCTCGATCATAAGGCCGTGCACTCGCTGATGTACGACA
TCATGAACGACAAACAGCGGCGCGATTACGAGGAGTTTCTCGAAACCGACTTTTCGTTTGCTCTGCCCGGCGTCGCGCGC
TTCCGGGTCAACGCCTTCAATCAGGAACGCGGCGCGGCGGGGGTTTTCCGGACCATTCCGTCAAAGGTTTTGAGCCTCGA
AGACCTGAAGGCGCCGAAGTTCTTCGAAGAAGTGACGCGCAAACCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CCGGCAAATCGACCACGCTGGCGGCGATGATCAACCACATCAACACGAACGATTACGCGCATATCCTGACCGTCGAAGAT
CCGATCGAATTCGTGCACGAAAGCCAGAAATGCCTGATCAACCAGCGCGAGGTACACCGCGACACGCACGGCTTCAACGA
GGCCTTGCGTTCGGCCTTGCGGGAAGACCCGGACATCATCCTGGTCGGCGAAATGCGCGACCTCGAAACGATCCGCCTCG
CACTGACCGCGGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACGACTTCCGCCGCGAAAACGATCGACCGGATC
ATCGACGTGTTTCCGGCCGCCGAAAAGGACATGATCCGCTCGATGCTGTCCGAATCGCTGCAGGCGGTCATTTCGCAAAC
GCTGCTCAAAAAAGTCGGCGGCGGCCGTATCGCGGCGCACGAGATCATGGTCGGCACCGCCGCGATCCGCAACCTGATCC
GCGAGGCGAAAGTCGCGCAAATGTATTCGGCGATTCAGACCGGACGCAAGGACGGCATGCAGACGCTCGACCAGAACCTG
AAAGAACTGGTCGACAAGGGGATGATCACCGCCAAAACCGCGGCGACCCGCGCCGTCAACAAGGACATGTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baylyi ADP1

79.472

98.841

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.681

0.678
  pilT Vibrio cholerae strain A1552

69.436

97.681

0.678
  pilT Neisseria meningitidis 8013

66.764

99.42

0.664
  pilT Neisseria gonorrhoeae MS11

66.472

99.42

0.661
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Pseudomonas stutzeri DSM 10701

40.173

100

0.403
  pilU Acinetobacter baylyi ADP1

39.769

100

0.4
  pilU Vibrio cholerae strain A1552

38.806

97.101

0.377

Multiple sequence alignment