Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEPPP_RS08855 Genome accession   NZ_CP169217
Coordinates   2013005..2014138 (+) Length   377 a.a.
NCBI ID   WP_374090708.1    Uniprot ID   -
Organism   Methylomicrobium lacus strain 22M6SE5-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2008005..2019138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPPP_RS08830 (ACEPPP_08830) tusD 2009259..2009648 (+) 390 WP_374090702.1 sulfurtransferase complex subunit TusD -
  ACEPPP_RS08835 (ACEPPP_08835) tusC 2009655..2010011 (+) 357 WP_374090703.1 sulfurtransferase complex subunit TusC -
  ACEPPP_RS08840 (ACEPPP_08840) - 2010434..2011114 (+) 681 WP_374090704.1 hypothetical protein -
  ACEPPP_RS08845 (ACEPPP_08845) - 2011132..2011821 (-) 690 WP_374090705.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEPPP_RS08850 (ACEPPP_08850) pilT 2011945..2012982 (+) 1038 WP_374090707.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEPPP_RS08855 (ACEPPP_08855) pilU 2013005..2014138 (+) 1134 WP_374090708.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEPPP_RS08860 (ACEPPP_08860) panD 2014237..2014623 (-) 387 WP_374090709.1 aspartate 1-decarboxylase -
  ACEPPP_RS08865 (ACEPPP_08865) panC 2014790..2015632 (-) 843 WP_374090710.1 pantoate--beta-alanine ligase -
  ACEPPP_RS08870 (ACEPPP_08870) panB 2015635..2016426 (-) 792 WP_374090711.1 3-methyl-2-oxobutanoate hydroxymethyltransferase -
  ACEPPP_RS08875 (ACEPPP_08875) folK 2016428..2016931 (-) 504 WP_374090712.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  ACEPPP_RS08880 (ACEPPP_08880) pcnB 2017022..2018401 (-) 1380 WP_374090713.1 polynucleotide adenylyltransferase PcnB -
  ACEPPP_RS08885 (ACEPPP_08885) - 2018618..2018794 (+) 177 WP_374090715.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41998.43 Da        Isoelectric Point: 6.2896

>NTDB_id=1049852 ACEPPP_RS08855 WP_374090708.1 2013005..2014138(+) (pilU) [Methylomicrobium lacus strain 22M6SE5-12]
MDFKALLALMVQKKASDLFITSGRAPTIKVDGSMVEVSKTPLTPEQAMKIVLSIMNQRQKDEFDNTKECQFAISLPDLGR
FRVSAFVQRDAAGMVLRRIENTIPDAETLHLPPVLKDLVMEKRGLVIFVGGTGTGKSTSLAALIKHRNQHTSGHIITIED
PMEFEHPHLGCIVTQREVGVDTESYEVALKNTLRQAPDVILIGEIRTRETMQNAITFAETGHLCLSTLHANNANQAIDRI
LHFFPEEMHPQLFMDLSLNLRGIVAQQLIKRADGKGRYPAIEILINTPLATDLIRKGEVHKLKELMKQSREQGMQTFDQA
LYDLYVAGKISYEDALNSADSKNEVRLMIKLGAEGISSTAGDNMMLTETEEDTGKLF

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1049852 ACEPPP_RS08855 WP_374090708.1 2013005..2014138(+) (pilU) [Methylomicrobium lacus strain 22M6SE5-12]
ATGGACTTCAAAGCCTTGCTCGCACTGATGGTGCAAAAGAAAGCCTCCGACCTGTTCATTACCTCAGGCCGCGCGCCGAC
GATCAAGGTCGACGGTTCGATGGTCGAAGTCTCCAAAACCCCGCTGACGCCCGAGCAGGCGATGAAAATCGTGCTCAGCA
TCATGAATCAGCGGCAAAAGGACGAGTTCGACAATACCAAGGAATGCCAGTTCGCGATCAGCCTGCCCGACCTCGGCCGT
TTCCGGGTCAGCGCCTTCGTGCAACGCGACGCGGCCGGCATGGTCTTGCGCCGGATCGAAAACACCATTCCCGACGCCGA
AACGCTGCACCTGCCGCCGGTGTTGAAAGATCTGGTCATGGAAAAACGCGGTCTGGTGATCTTCGTCGGCGGCACCGGCA
CCGGTAAGTCGACCTCGCTGGCCGCGCTGATAAAACACCGCAACCAACACACCAGCGGCCACATCATCACGATCGAAGAC
CCGATGGAATTCGAGCATCCGCACTTAGGCTGCATCGTGACCCAGCGCGAAGTCGGCGTCGATACCGAATCGTATGAAGT
CGCGTTGAAAAACACCCTGCGGCAGGCGCCCGACGTGATCCTGATCGGCGAAATAAGGACCCGCGAAACGATGCAGAACG
CAATCACCTTCGCCGAAACCGGACACCTGTGCCTATCGACCCTGCACGCGAACAATGCGAACCAGGCGATCGACCGGATC
CTGCATTTCTTTCCCGAGGAAATGCATCCGCAGCTGTTCATGGACCTGTCGCTGAACCTGCGCGGCATCGTCGCCCAGCA
ATTGATCAAGCGCGCGGACGGCAAGGGCCGTTATCCGGCGATCGAAATCCTGATCAACACGCCGCTGGCGACGGATTTGA
TCCGCAAGGGCGAGGTGCACAAACTGAAGGAATTGATGAAGCAATCGCGCGAACAGGGCATGCAGACCTTCGATCAGGCC
TTGTACGATCTGTATGTCGCCGGCAAGATCAGCTATGAGGATGCATTGAATTCGGCCGACTCGAAGAACGAAGTCCGCCT
GATGATCAAGCTCGGCGCCGAAGGCATCAGCAGCACGGCCGGCGACAACATGATGCTGACCGAGACCGAAGAGGATACAG
GCAAGTTGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.947

100

0.645
  pilU Acinetobacter baylyi ADP1

62.079

94.43

0.586
  pilU Vibrio cholerae strain A1552

57.143

92.838

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.103

92.308

0.398
  pilT Legionella pneumophila strain Lp02

41.983

90.981

0.382
  pilT Legionella pneumophila strain ERS1305867

41.983

90.981

0.382
  pilT Pseudomonas aeruginosa PAK

41.003

89.92

0.369

Multiple sequence alignment