Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACEPA4_RS15780 Genome accession   NZ_CP169216
Coordinates   3412948..3413382 (-) Length   144 a.a.
NCBI ID   WP_070490391.1    Uniprot ID   A0AAP7J1T7
Organism   Stenotrophomonas sp. P2112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3404887..3431300 3412948..3413382 within 0


Gene organization within MGE regions


Location: 3404887..3431300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPA4_RS15745 (ACEPA4_15745) - 3404887..3406101 (+) 1215 WP_219634362.1 glycosyltransferase family 4 protein -
  ACEPA4_RS15750 (ACEPA4_15750) - 3406145..3407341 (+) 1197 WP_235643011.1 lipopolysaccharide biosynthesis protein -
  ACEPA4_RS15755 (ACEPA4_15755) - 3407304..3408419 (-) 1116 WP_111202054.1 glycosyltransferase -
  ACEPA4_RS15760 (ACEPA4_15760) - 3408416..3409228 (-) 813 WP_374082884.1 FkbM family methyltransferase -
  ACEPA4_RS15765 (ACEPA4_15765) - 3409386..3410489 (+) 1104 WP_111202056.1 glycosyltransferase family 4 protein -
  ACEPA4_RS15770 (ACEPA4_15770) - 3410523..3410939 (-) 417 WP_070490395.1 pilin -
  ACEPA4_RS15775 (ACEPA4_15775) - 3410943..3412868 (-) 1926 WP_146257844.1 hypothetical protein -
  ACEPA4_RS15780 (ACEPA4_15780) pilE 3412948..3413382 (-) 435 WP_070490391.1 pilin Machinery gene
  ACEPA4_RS15785 (ACEPA4_15785) pilC 3413738..3414994 (+) 1257 WP_070490390.1 type II secretion system F family protein Machinery gene
  ACEPA4_RS15790 (ACEPA4_15790) - 3415002..3415865 (+) 864 WP_065185263.1 A24 family peptidase -
  ACEPA4_RS15795 (ACEPA4_15795) coaE 3415877..3416488 (+) 612 WP_374082885.1 dephospho-CoA kinase -
  ACEPA4_RS15800 (ACEPA4_15800) - 3416568..3416954 (-) 387 WP_279945997.1 hypothetical protein -
  ACEPA4_RS15805 (ACEPA4_15805) - 3417484..3418854 (-) 1371 WP_061479297.1 HAMP domain-containing sensor histidine kinase -
  ACEPA4_RS15810 (ACEPA4_15810) - 3418817..3419494 (-) 678 WP_005410804.1 response regulator transcription factor -
  ACEPA4_RS15815 (ACEPA4_15815) - 3419657..3420118 (-) 462 WP_065178936.1 hypothetical protein -
  ACEPA4_RS15820 (ACEPA4_15820) - 3420234..3420704 (-) 471 WP_005418647.1 hypothetical protein -
  ACEPA4_RS15825 (ACEPA4_15825) - 3420799..3421278 (-) 480 WP_005418649.1 hypothetical protein -
  ACEPA4_RS15830 (ACEPA4_15830) rimK 3421537..3422415 (-) 879 WP_005418651.1 30S ribosomal protein S6--L-glutamate ligase -
  ACEPA4_RS15835 (ACEPA4_15835) - 3422513..3422755 (-) 243 WP_005418653.1 hypothetical protein -
  ACEPA4_RS15840 (ACEPA4_15840) - 3422901..3423404 (+) 504 WP_139727565.1 hypothetical protein -
  ACEPA4_RS15845 (ACEPA4_15845) - 3423707..3423928 (-) 222 WP_010481480.1 DUF2188 domain-containing protein -
  ACEPA4_RS15850 (ACEPA4_15850) - 3424431..3425675 (-) 1245 WP_065185271.1 HlyD family secretion protein -
  ACEPA4_RS15855 (ACEPA4_15855) - 3425672..3427792 (-) 2121 WP_239499552.1 peptidase domain-containing ABC transporter -
  ACEPA4_RS15860 (ACEPA4_15860) - 3428153..3428800 (-) 648 WP_231109041.1 type II CAAX prenyl endopeptidase Rce1 family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14643.86 Da        Isoelectric Point: 9.6276

>NTDB_id=1049838 ACEPA4_RS15780 WP_070490391.1 3412948..3413382(-) (pilE) [Stenotrophomonas sp. P2112]
MKNQKGFTLIELMIVVAIIAILAAIALPAYQDYVARSQVSEAMTLSSGAKTAVTEYYADKGAFPTTNALAGLAGASSISG
KYVTSVTVGTGGAITAMMKGSGSVSSKVAGKTFVLTPADAGGSITWDCKSSGTIDAKYRPSSCR

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1049838 ACEPA4_RS15780 WP_070490391.1 3412948..3413382(-) (pilE) [Stenotrophomonas sp. P2112]
ATGAAGAACCAGAAGGGCTTCACCCTTATCGAACTGATGATCGTTGTTGCGATCATCGCCATCCTGGCTGCCATTGCGCT
GCCGGCTTACCAGGACTATGTGGCACGCTCGCAGGTTTCCGAAGCGATGACCCTGAGCTCGGGCGCCAAGACCGCTGTTA
CCGAGTACTACGCCGACAAGGGCGCCTTCCCGACCACCAATGCCTTGGCTGGCCTGGCTGGCGCCTCGTCGATCAGCGGC
AAGTACGTGACCAGCGTGACTGTTGGCACCGGTGGTGCAATTACCGCAATGATGAAGGGCTCGGGTTCGGTTAGCTCCAA
GGTTGCAGGCAAGACCTTCGTGCTGACTCCGGCTGATGCGGGCGGCTCGATCACCTGGGATTGCAAGAGCTCCGGTACCA
TCGACGCCAAGTACCGTCCGTCGTCCTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

49.045

100

0.535
  pilE Neisseria gonorrhoeae strain FA1090

48.718

100

0.528
  pilA/pilA1 Eikenella corrodens VA1

44.304

100

0.486
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.043

100

0.486
  pilA2 Legionella pneumophila str. Paris

48.592

98.611

0.479
  comP Acinetobacter baylyi ADP1

46.309

100

0.479
  pilA2 Legionella pneumophila strain ERS1305867

47.887

98.611

0.472
  pilA Ralstonia pseudosolanacearum GMI1000

38.182

100

0.438
  pilA Acinetobacter baumannii strain A118

42.466

100

0.431
  pilA Pseudomonas aeruginosa PAK

38.816

100

0.41
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.844

97.917

0.41
  comF Acinetobacter baylyi ADP1

38.255

100

0.396
  pilA Acinetobacter nosocomialis M2

46.341

85.417

0.396
  pilA Haemophilus influenzae 86-028NP

39.716

97.917

0.389
  pilA Vibrio campbellii strain DS40M4

36.913

100

0.382
  pilA Haemophilus influenzae Rd KW20

39.007

97.917

0.382
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.857

97.222

0.368

Multiple sequence alignment