Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEPM3_RS13990 Genome accession   NZ_CP168727
Coordinates   2946200..2947243 (-) Length   347 a.a.
NCBI ID   WP_011002592.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN6499     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2941200..2952243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM3_RS13960 (ACEPM3_13960) - 2941729..2942265 (-) 537 WP_011002586.1 PilX N-terminal domain-containing pilus assembly protein -
  ACEPM3_RS13965 (ACEPM3_13965) - 2942269..2943297 (-) 1029 WP_016723683.1 PilW family protein -
  ACEPM3_RS13970 (ACEPM3_13970) pilV 2943294..2943884 (-) 591 WP_011002588.1 type IV pilus modification protein PilV -
  ACEPM3_RS13975 (ACEPM3_13975) - 2943881..2944369 (-) 489 WP_016723684.1 Tfp pilus assembly protein FimT/FimU -
  ACEPM3_RS13980 (ACEPM3_13980) - 2944373..2944813 (-) 441 WP_011002590.1 type IV pilin protein -
  ACEPM3_RS13985 (ACEPM3_13985) pilU 2945008..2946147 (-) 1140 WP_011002591.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEPM3_RS13990 (ACEPM3_13990) pilT 2946200..2947243 (-) 1044 WP_011002592.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEPM3_RS13995 (ACEPM3_13995) - 2947368..2948090 (+) 723 WP_011002593.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEPM3_RS14000 (ACEPM3_14000) proC 2948125..2948949 (+) 825 WP_011002594.1 pyrroline-5-carboxylate reductase -
  ACEPM3_RS14005 (ACEPM3_14005) - 2948971..2949249 (+) 279 WP_011002595.1 hypothetical protein -
  ACEPM3_RS14010 (ACEPM3_14010) ubiA 2949246..2950121 (-) 876 WP_011002596.1 4-hydroxybenzoate octaprenyltransferase -
  ACEPM3_RS14015 (ACEPM3_14015) - 2950195..2950695 (-) 501 WP_003271865.1 Dps family protein -
  ACEPM3_RS14020 (ACEPM3_14020) - 2950854..2951096 (-) 243 WP_011002597.1 hypothetical protein -
  ACEPM3_RS14025 (ACEPM3_14025) - 2951155..2951403 (-) 249 WP_019718289.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38150.80 Da        Isoelectric Point: 6.8705

>NTDB_id=1045329 ACEPM3_RS13990 WP_011002592.1 2946200..2947243(-) (pilT) [Ralstonia pseudosolanacearum strain RUN6499]
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1045329 ACEPM3_RS13990 WP_011002592.1 2946200..2947243(-) (pilT) [Ralstonia pseudosolanacearum strain RUN6499]
ATGGACATCGCGCAGCTTCTGGCCTTCGCTGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCTCCGAT
GATCCGGATCCATGGCGACATGCGCCGTATCAACGTGCCGCCGCTCACGCACCAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCCGACTTCTCGTTCGAGATTCCCGGGCTGTCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGTGCCGCCGCCGTGTTCCGGACGATTCCGTCCAAGGTCCTGACGCTGGA
AGACCTGAAGGCGCCGGCCGTCTTCGCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACGGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAATCACCGCAATGAAAGCGACCTGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAACTGGGGCCGCATACCCATTCGTTTGCCAA
CGCGCTGAAATCGGCGCTGCGGGAAGACCCGGACGTGGTCTTGGTCGGCGAATTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAAACCGGCCACTTGGTGTTTGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCGGATGAGAAGGACATGGTCCGCACCATGTTGTCCGAATCGCTGGAAGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATCTGCACGCCGGCCATCCGACACCTGA
TCCGCGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGGCGGAGCTCATCAAGCGCTCGGCGATCAACTACGCCGACGCGCGTGCCATCGCCAAGAACCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.594

99.424

0.712
  pilT Acinetobacter baumannii D1279779

71.594

99.424

0.712
  pilT Acinetobacter baumannii strain A118

71.594

99.424

0.712
  pilT Pseudomonas aeruginosa PAK

71.304

99.424

0.709
  pilT Pseudomonas stutzeri DSM 10701

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

69.855

99.424

0.695
  pilT Neisseria gonorrhoeae MS11

68.497

99.712

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Legionella pneumophila strain Lp02

67.826

99.424

0.674
  pilT Legionella pneumophila strain ERS1305867

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.118

0.643
  pilT Vibrio cholerae strain A1552

66.172

97.118

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.867

99.712

0.507
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403

Multiple sequence alignment