Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEPM3_RS13985 Genome accession   NZ_CP168727
Coordinates   2945008..2946147 (-) Length   379 a.a.
NCBI ID   WP_011002591.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN6499     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2940008..2951147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM3_RS13960 (ACEPM3_13960) - 2941729..2942265 (-) 537 WP_011002586.1 PilX N-terminal domain-containing pilus assembly protein -
  ACEPM3_RS13965 (ACEPM3_13965) - 2942269..2943297 (-) 1029 WP_016723683.1 PilW family protein -
  ACEPM3_RS13970 (ACEPM3_13970) pilV 2943294..2943884 (-) 591 WP_011002588.1 type IV pilus modification protein PilV -
  ACEPM3_RS13975 (ACEPM3_13975) - 2943881..2944369 (-) 489 WP_016723684.1 Tfp pilus assembly protein FimT/FimU -
  ACEPM3_RS13980 (ACEPM3_13980) - 2944373..2944813 (-) 441 WP_011002590.1 type IV pilin protein -
  ACEPM3_RS13985 (ACEPM3_13985) pilU 2945008..2946147 (-) 1140 WP_011002591.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEPM3_RS13990 (ACEPM3_13990) pilT 2946200..2947243 (-) 1044 WP_011002592.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEPM3_RS13995 (ACEPM3_13995) - 2947368..2948090 (+) 723 WP_011002593.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEPM3_RS14000 (ACEPM3_14000) proC 2948125..2948949 (+) 825 WP_011002594.1 pyrroline-5-carboxylate reductase -
  ACEPM3_RS14005 (ACEPM3_14005) - 2948971..2949249 (+) 279 WP_011002595.1 hypothetical protein -
  ACEPM3_RS14010 (ACEPM3_14010) ubiA 2949246..2950121 (-) 876 WP_011002596.1 4-hydroxybenzoate octaprenyltransferase -
  ACEPM3_RS14015 (ACEPM3_14015) - 2950195..2950695 (-) 501 WP_003271865.1 Dps family protein -
  ACEPM3_RS14020 (ACEPM3_14020) - 2950854..2951096 (-) 243 WP_011002597.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42237.78 Da        Isoelectric Point: 6.9274

>NTDB_id=1045328 ACEPM3_RS13985 WP_011002591.1 2945008..2946147(-) (pilU) [Ralstonia pseudosolanacearum strain RUN6499]
MLDRDAATRYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMSEKQLREFEESFECNFA
ITAPTAGRFRVSAFMQQGRAGMVLRTITTKIPTLAELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGKKGRAPAIEILLGTPLVSDLIFKGEVHELKEVMKKSREQ
GMVCFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1045328 ACEPM3_RS13985 WP_011002591.1 2945008..2946147(-) (pilU) [Ralstonia pseudosolanacearum strain RUN6499]
ATGCTGGACCGCGACGCCGCCACCAGATATATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACCGCGGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGCTGACCACCGTCC
AGGCCATGGGCCTGGTCAAGGCGATCATGAGCGAGAAGCAGCTGCGCGAGTTCGAAGAGTCGTTCGAGTGCAACTTCGCC
ATCACCGCGCCCACCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CACCACCAAGATCCCGACGCTCGCCGAACTGGATCTGCCGCCCATCCTCAACGAAGTCGCGATGAGCAAGCGCGGGCTCG
TGGTGGTGGTGGGGGCGACGGGCTCCGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTAC
GGCCACATCATCACCATCGAAGACCCGGTGGAATATGTGCACGCACACCACAACTGCGTCGTGACGCAGCGCGAGGTGGG
CGTGGACACGGAATCCTGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGATACGATGGAGTACGCCATCCAGTACGCCGAAACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGTCAGCAGTTGCTGATCGATCTGTCCCTGAA
CCTGCGCGCCATGATCGCGCAGCGCCTGCTGCCGCGCAAAGGCAAGAAGGGCCGCGCGCCGGCGATCGAAATCCTGCTGG
GCACGCCGCTGGTGTCCGACCTGATCTTCAAGGGCGAGGTGCACGAGCTCAAGGAGGTCATGAAGAAGTCGCGCGAGCAG
GGCATGGTCTGTTTCGACCAGGCGCTGTTCGAGCTCTACGAGGCGGACAAGATCACCTATGAGGATGCGCTGCGCAACGC
GGATTCGCTGAACGACCTGCGTCTGCAGATCAAGCTGCATAGCAAGCGCGGCGGGCCGGTGGATCTGACGGCCGGCACTG
AGCATCTCAACGTGATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.323

93.14

0.58
  pilU Acinetobacter baylyi ADP1

55.432

94.723

0.525
  pilU Vibrio cholerae strain A1552

55.367

93.404

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

88.918

0.391
  pilT Legionella pneumophila strain Lp02

43.544

87.863

0.383
  pilT Legionella pneumophila strain ERS1305867

43.544

87.863

0.383
  pilT Vibrio cholerae strain A1552

45.426

83.641

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.426

83.641

0.38
  pilT Pseudomonas aeruginosa PAK

42.73

88.918

0.38
  pilT Acinetobacter baylyi ADP1

44.41

84.96

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.543

88.918

0.369
  pilT Acinetobacter baumannii D1279779

43.574

84.169

0.367
  pilT Acinetobacter baumannii strain A118

43.574

84.169

0.367
  pilT Acinetobacter nosocomialis M2

43.574

84.169

0.367

Multiple sequence alignment