Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEN9T_RS11675 Genome accession   NZ_CP168610
Coordinates   639484..640527 (+) Length   347 a.a.
NCBI ID   WP_009241469.1    Uniprot ID   A0A191ZZJ8
Organism   Ralstonia sp. Ralssp110 strain CT11-110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 634484..645527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9T_RS11655 (ACEN9T_11660) katG 634616..636793 (+) 2178 WP_413707778.1 catalase/peroxidase HPI -
  ACEN9T_RS11660 (ACEN9T_11665) ubiA 636860..637735 (+) 876 WP_413707779.1 4-hydroxybenzoate octaprenyltransferase -
  ACEN9T_RS11665 (ACEN9T_11670) proC 637790..638614 (-) 825 WP_413707780.1 pyrroline-5-carboxylate reductase -
  ACEN9T_RS11670 (ACEN9T_11675) - 638645..639361 (-) 717 WP_413707781.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEN9T_RS11675 (ACEN9T_11680) pilT 639484..640527 (+) 1044 WP_009241469.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEN9T_RS11680 (ACEN9T_11685) pilU 640595..641734 (+) 1140 WP_092972473.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN9T_RS11685 (ACEN9T_11690) - 641932..642369 (+) 438 WP_413707782.1 type IV pilin protein -
  ACEN9T_RS11690 (ACEN9T_11695) - 642378..642851 (+) 474 WP_045202622.1 GspH/FimT family pseudopilin -
  ACEN9T_RS11695 (ACEN9T_11700) pilV 642848..643450 (+) 603 WP_413707783.1 type IV pilus modification protein PilV -
  ACEN9T_RS11700 (ACEN9T_11705) - 643447..644484 (+) 1038 WP_092972476.1 PilW family protein -
  ACEN9T_RS11705 (ACEN9T_11710) - 644487..645056 (+) 570 WP_092972477.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38193.80 Da        Isoelectric Point: 6.8307

>NTDB_id=1044606 ACEN9T_RS11675 WP_009241469.1 639484..640527(+) (pilT) [Ralstonia sp. Ralssp110 strain CT11-110]
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDGQRKVYEENLEVDFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKEMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMIATPAIRHLIRENKISQMYSMMQTSSGMGMQTLDQC
LSELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1044606 ACEN9T_RS11675 WP_009241469.1 639484..640527(+) (pilT) [Ralstonia sp. Ralssp110 strain CT11-110]
ATGGACATCGCGCAGCTTCTGGCGTTTTCCGCCAAGAACAAGGCGTCGGACTTGCACCTGTCGGCCGGACTGCCGCCGAT
GATCCGGATCCACGGCGACATGCGCCGGATCAACGTGCCACCGCTCACGCACCAAGATGTGCACTCCATGGTGTACGACA
TCATGAGCGACGGGCAGCGCAAGGTCTACGAAGAAAACCTGGAAGTCGACTTCTCGTTTGAGATCCCCGGCCTCTCGCGT
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCTTCGGCCGTATTCCGGACGATTCCGTCGAAGGTGCTCTCGCTGGA
CGACCTGAAGGCGCCTGCCGTGTTTGCGGACTTGGCGATGAAGCCGCGCGGCCTGGTGCTCGTCACGGGCCCGACTGGCT
CGGGCAAGTCGACGACGCTTGCCGCGATGGTCAACCACCGCAACGAAAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCATGAATCGAAGAAGAGCCTGATCAACCAGCGCGAGCTTGGTCCGCATACACACTCGTTCGCCAA
TGCGCTGAAGTCGGCGCTGCGTGAAGACCCGGACGTGGTGCTGGTGGGCGAATTGCGTGATCTTGAGACCATTCGCCTGG
CGCTCACGGCCGCAGAAACGGGCCACTTGGTGTTCGGCACGCTGCACACCAGCTCGGCTGCCAAGACCATCGACCGGGTC
GTCGACGTGTTTCCGTCGGACGAAAAAGAGATGGTCCGGACCATGCTTTCCGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGGTCGGGCCGGATTGCGGCGCATGAAATCATGATTGCCACGCCGGCCATCCGTCACTTGA
TCCGCGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACGAGCAGCGGCATGGGCATGCAGACGCTCGACCAATGT
CTGTCGGAGCTGATCAAGCGCTCGGCGATCAACTACGCAGACGCGCGCGCCATCGCCAAGAACCCGGACGCGTTCGCGAA
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191ZZJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.014

99.424

0.706
  pilT Acinetobacter baumannii D1279779

71.014

99.424

0.706
  pilT Acinetobacter baumannii strain A118

71.014

99.424

0.706
  pilT Pseudomonas aeruginosa PAK

70.145

99.424

0.697
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.424

0.695
  pilT Acinetobacter baylyi ADP1

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.786

99.712

0.686
  pilT Neisseria meningitidis 8013

68.786

99.712

0.686
  pilT Legionella pneumophila strain Lp02

66.957

99.424

0.666
  pilT Legionella pneumophila strain ERS1305867

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.118

0.643
  pilT Vibrio cholerae strain A1552

66.172

97.118

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.289

99.712

0.501
  pilU Acinetobacter baylyi ADP1

42.733

99.135

0.424
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395

Multiple sequence alignment